Fluorescence PCR (Polymerase Chain Reaction) detection kit for group B streptococcus

Abstract

The invention discloses a fluorescence PCR (Polymerase Chain Reaction) detection kit for group B streptococcus. The fluorescence PCR detection kit comprises the following components: a PCR reaction liquid, an enzyme mixed liquid, a group B streptococcus reaction liquid, an internal reference plasmid, a positive reference and a negative reference. The fluorescence PCR detection kit is used for overcoming the defects that more time is wasted by using a gold standard bacterial culture method, a serological diagnosis method has hysteresis and a gene sequence determination method is more complex. The fluorescence PCR detection kit has the advantages of high sensitivity, good specificity, strong repeatability, rapid and objective detection result and the like so as to have a wide application prospect in the field of in-vitro diagnosis of the group B streptococcus.

Description

technical field [0001] The invention relates to a fluorescent PCR detection kit, in particular to a group B streptococcus fluorescent PCR detection kit, which belongs to the field of biotechnology. Background technique [0002] Group B streptococcus (GBS) is an opportunistic pathogen that parasitizes in the human genitourinary tract and lower gastrointestinal tract, and the carrier rate of healthy people can reach 35%. As early as the 1970s, GBS had been confirmed as one of the important pathogenic bacteria for perinatal maternal and infant infection. Group B streptococcus colonized in the maternal genital tract can be vertically transmitted to newborns during delivery, and neonatal sepsis, meningitis, and pneumonia caused by GBS infection in newborns have a high fatality rate and can lead to neurological sequelae . In 2010, the US Centers for Disease Control and Prevention formulated the "Guidelines for Perinatal GBS Prevention", recommending that pregnant women undergo G...

AI Technical Summary

Problems solved by technology

This method is low in cost, is greatly affected by human factors, and takes a long time to cultivate, so it is not suitable for current clinical diagnosis

[0006] 2) Serological methods cannot process samples with high throughput

[0008] It can be seen that the bacterial culture method as the gold standard is time-consuming, and there is a lag in serological diagnostic methods.

In addition, gene sequence determination methods are relatively complex and cumbersome

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