Method for discriminating types of propolis raw materials

A technology for propolis and raw materials, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of cumbersome and time-consuming detection process, expensive detection cost, and expensive and precise instruments, and achieves a clear and definite judgment result, an easy judgment method and a high correct judgment rate. Effect

Active Publication Date: 2013-11-27
BEE RES INST CHINESE ACAD OF AGRI SCI
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the instruments used in the above studies are precise and expensive, and require extremely high levels of testing personnel. The testing process is cumbersome and time-consuming, and the testing costs are expensive, so it is not suitable for application a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for discriminating types of propolis raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Take six samples of propolis raw materials (coarse glue) whose glue source plants are poplar, birch, eucalyptus, rubber, Brazilian propolis, and artificially boiled poplar gum, place them in a -10°C refrigerator for 24 hours, and use a leather hammer After crushing, it is crushed in a 2000r / min high-speed pulverizer;

[0037] Accurately weigh 2.0g of each pulverized sample into a centrifuge tube, add 50ml of absolute ethanol, shake on a rotary shaker for 2 hours, ultrasonically extract for 1 hour, centrifuge at low temperature and high speed at 0°C and 10,000r / min for 10min, and use a pipette to remove the supernatant Transfer to a transparent disposable plastic petri dish (specification 9cm), and place a piece of white filter paper under the petri dish;

[0038] The L*a*b* values ​​of propolis raw material alcohol solutions were measured with the help of a colorimeter, and the measured data values ​​were substituted into the six glue source discrimination models of the...

Embodiment 2

[0049]The propolis sample is a domestic sample. It is known that its type is one of the above six types, but the specific type of glue source is unknown. The number is FJ-1. Grinding in a high-speed pulverizer, passing the crushed sample through a 20-mesh sieve; accurately weighing 2.0g into a centrifuge tube, adding 50mL of absolute ethanol, shaking on a rotary shaker for 2h, ultrasonic extraction for 1h, and low temperature at 0°C and 10000r / min Centrifuge at high speed for 10 minutes, transfer the supernatant to a transparent disposable plastic petri dish (9cm in size) with a straw, and place a piece of white filter paper under the petri dish;

[0050] The L*, a*, and b* values ​​of the FJ-1 alcohol solution were measured with the help of a colorimeter, and the measured data values ​​were substituted into the linear discriminant model of the six types of propolis. The results are shown in Table 2.

[0051] Comparing the size of the 6 Y values, the maximum value is the calcu...

Embodiment 3

[0053] The known type of propolis is one of the above six types, but the specific source of propolis is unknown, numbered FJ-2, placed in a -10°C refrigerator for 5 hours, crushed with a hammer, and crushed at a high speed of 2000r / min Grinding in the machine, passing the crushed sample through a 20-mesh sieve; accurately weighing 2.0g into a centrifuge tube, adding 50mL of absolute ethanol, shaking on a rotary shaker for 2h, ultrasonically extracting for 1h, and centrifuging at low temperature and high speed for 10min at 0°C and 10,000r / min , transfer the supernatant to a transparent disposable plastic petri dish (specification 9cm) with a straw, and place a piece of white filter paper under the petri dish;

[0054] The L*, a*, and b* values ​​of the propolis raw material alcohol solution were measured with the help of a colorimeter, and the measured data values ​​were substituted into the discriminant model formulas (1) to (6) in Example 1 for calculation. The results are sho...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for discriminating types of propolis raw materials. The method comprises the following steps: (1) crushing a propolis sample to be detected, dissolving the sample in absolute ethyl alcohol, and preparing a mixture, wherein the mass and volume ratio of the sample to the absolute ethyl alcohol is 1:25; (2) shaking the mixture for 1.5-3 hours, performing ultrasonic treatment for 1-1.5 hours, performing centrifugal separation at low temperature, and taking the separated liquid; and (3) measuring a chromatic value of the separated liquid by using a colorimeter, substituting in a linear discriminant model to calculate a Y value, and discriminating the sample to be detected as a propolis source type with the maximum type. The provided method is easy to operate, has low requirements on instruments and levels of detectors and is easily popularized in actual production; the accurate discrimination rate is high; the discrimination range of propolis source plants is wide, and common propolis source types in the propolis raw material market in China are almost covered; the measurement result is measurable and is not influenced by subjective judgment of the detectors; moreover, the discrimination result is clear and is easily standardized.

Description

technical field [0001] The invention relates to a method for identifying varieties of bee products, in particular to a method for identifying types of propolis raw materials. Background technique [0002] Propolis is an aromatic viscous solid that is mixed with secretions such as resin collected by bees from buds, bark or stem wounds of plants, mixed with part of beeswax and pollen, and secretions of bee maxillary glands. The chemical composition of propolis is complex, rich in flavonoids, terpenes, phenolic acids and other bioactive substances. A large number of modern physiological and pharmacological studies have shown that propolis has various biological activities such as antibacterial, antiviral, antioxidative, anti-aging, antitumor, anti-inflammatory, enhancing immunity, lowering blood sugar and blood lipids, sedation, and anesthesia. Therefore, propolis has become a hot spot in the research and development of bee products in recent ten years, and has been more and m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/27
Inventor 吴黎明王勇陈芳薛晓锋陈兰珍李熠
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap