Method for jugular vein blood collection of guinea pig

A jugular vein and guinea pig technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of difficult repeated collection, limited blood collection volume, difficult operation, etc., and achieve the effect of not easy to pollute, promote development, and repeat collection

Inactive Publication Date: 2013-12-18
WUXI APPTEC SUZHOU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can avoid the disadvantages of large damage, easy contamination, difficult operation, difficult repeated collection, and limited blood collection volume.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] Guinea pig jugular vein blood sampling method of the present invention, comprises the steps:

[0023] 1) Baoding Guinea Pig

[0024] After checking the number (ID number) of the guinea pig, secure the limbs of the guinea pig on the baling platform, use the headgear to secure the head of the guinea pig, gently rotate and push to one side, so that the body and head of the guinea pig form a certain angle; the other side appears A triangular area bounded by the muscles of the head and forelimbs.

[0025] When baoding, pay attention to the tightness of the baoding to ensure that the guinea pig can breathe freely, and the keeper should always observe the breathing condition of the guinea pig.

[0026] 2) Before blood collection, shave the blood collection site of the guinea pig.

[0027] 3) Collect blood from the jugular vein of guinea pigs

[0028] Prepare a syringe according to the amount of blood collected. Use a 1mL or 2.5mL syringe at most. Insert the needle into the ...

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PUM

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Abstract

The invention discloses a method for jugular vein blood collection of a guinea pig. The method comprises the steps of 1) fixing the guinea pig; 2) shaving a blood collection portion; 3) collecting blood at a position of a jugular vein of the guinea pig; 4) after blood collection is finished, stopping bleeding. The blood collection method has the advantages that the operation is simple, the damage is small, blood is not prone to pollution and can be collected repeatedly and the like, and the method has a broad application value.

Description

technical field [0001] The invention relates to a method for venous blood collection, in particular to a method for guinea pig jugular vein blood collection. Background technique [0002] Guinea pigs are important experimental animals, often used in immunology, epidemiology, pharmacology, nutrition and otology research. However, due to its special physiological structure, there are certain difficulties in blood collection. Among them, the most widely used blood collection methods are as follows: [0003] 1) Blood collection through ear incision: [0004] Disinfect the ear of the guinea pig first, cut the edge of the ear with a blade along the direction of the blood vessel, the incision is about 0.5 cm long, and apply 20% sodium citrate solution on the edge of the incision to prevent hemagglutination, and blood can flow out from the incision. This method can take 0.5ml of blood each time. [0005] 2) Blood collection from the dorsal and midfoot veins: [0006] Fix the gui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
Inventor 梅敏袁海宏
Owner WUXI APPTEC SUZHOU
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