A rapid detection test strip of Fasciola large fasciola immunochromatography and its preparation method

A technology for detecting test strips and immunochromatography, which is applied in the directions of measuring devices, analytical materials, instruments, etc., can solve the problems of high price and complicated operation, and achieve the effects of simple operation, good effect and wide application range.

Active Publication Date: 2016-10-05
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a rapid detection test strip of fascioliasis immunochromatography and its preparation method, which can solve the problems of expensive and complicated operation of the existing equipment for detecting and diagnosing fascioliasis

Method used

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  • A rapid detection test strip of Fasciola large fasciola immunochromatography and its preparation method

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preparation example Construction

[0031] B. Preparation of rabbit anti-mouse immunoglobulin antibody: Mix mouse immunoglobulin with a mass concentration of 1 mg / mL with Freund's complete adjuvant 1:1, and subcutaneously apply 1 ml / rabbit to the paw of the rabbit. Injection immunization; 7 days later, the mouse IgG with a mass concentration of 2 mg / ml was mixed with incomplete Freund's adjuvant 1:1, and the above-mentioned rabbits were injected with a secondary immunization in the popliteal lymph node at a dose of 0.5 mL / rabbit; 10 Days later, the immunization was boosted once by the method of secondary immunization. After immunization, blood was collected from ear vein regularly to measure the serum titer. After the serum titer was reached, blood was collected from the heart, and the serum was separated; after preliminary purification by the conventional octanoic acid-saturated ammonium sulfate method, Then use the portion G protein column for further purification; when the titer of rabbit anti-mouse immunoglob...

Embodiment 1

[0046] The colloidal gold-labeled 7D1 monoclonal antibody against Fasciola maxima ES antigen prepared by the above method was diluted 1:5, and sprayed on the labeling pad in an amount of 12.5 μL per pad for coating, and then the labeling pad was baked at 37°C. Store dry and sealed.

[0047] The 7D4 monoclonal antibody against the ES antigen of Fasciola maxima was diluted to a concentration of 0.26 mg / mL, and the amount of 1.5 μL was coated on the coated detection line T; the rabbit anti-mouse immunoglobulin antibody was diluted to 0.30 mg / mL The concentration of 1.5μL was coated to the quality control line C of the coated film, dried at 37°C, and sealed for storage.

[0048] The sample pad 2, the marking pad 3, the coating film 4, and the water-absorbing pad 5 are lapped and pasted on the support liner made of PVC material in sequence, and the fasciola immunochromatographic rapid detection test strip is completed.

[0049] The immunochromatographic rapid detection test strip ...

Embodiment 2

[0051] Validation assay for detection sensitivity to ES antigens of Fasciola grandis. The test strip was prepared according to the preparation method of the rapid detection test strip for the detection of Fasciola maxima by immunochromatography described in Example 1. After purification of Fasciola maxima ES antigen, the antigen concentration was determined by UV spectrophotometer to be 0.94 gm / mL. : 12800 was double-diluted and detected with test strips, each dilution was detected with three test strips, and a parallel control was set up, the results are shown in Table 1.

[0052] Table 1 Test results of test strip detection sensitivity

[0053] Antigen dilution 200 400 800 1600 3200 6400 12800

[0054] Antigen concentration (μg) 47.00 2.35 1.18 0.59 0.29 ¬¬— —

[0055] T-line Color development Color development Color development Color development No color development No color development

[0056] C-line color rendering color rendering color rendering color rendering colo...

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Abstract

The invention discloses a rapid detection test strip of flax fluke immunochromatography, which relates to the technical field of biological detection, and includes a support liner, on which a coating film, a marker pad and a sample pad are sequentially pasted, on the support liner The hand-held end of the liner is pasted with a water-absorbing pad; the marker pad is coated with the 7D1 monoclonal antibody against the ES antigen of Fasciola macula, the front part of the coating film is provided with a detection line, and the rear part of the coating film is provided with a detection line. Quality control line, the detection line is coated with the 7D4 monoclonal antibody against the ES antigen of Fasciola large, and the quality control line is coated with rabbit anti-mouse immunoglobulin antibody. Compared with the prior art, the present invention The test strip is easy to operate, does not require professionals in the field and professional equipment and instruments, can meet the needs of personnel and departments at different levels, especially the needs of grassroots units, and has the advantages of rapidity, accuracy, sensitivity, specificity, and low price.

Description

technical field [0001] The invention relates to the technical field of biological monitoring, in particular to an immunochromatographic rapid detection test strip for rapid detection of fascioliasis and a preparation method thereof. Background technique [0002] Fasciola gigantica (Fasciola gigantica) is an important parasite that parasitizes humans and animals. It mainly harms cattle and sheep in livestock. After infecting cattle, it can cause acute or chronic hepatitis and cholangitis, leading to systemic poisoning and nutritional disorders. It will reduce the immunity of cattle, reduce the production of meat and milk, and reduce the quality. In the case of severe infection, it can also cause a large number of deaths, bringing huge economic losses to animal husbandry and agriculture; Fascioliasis infection can also cause serious parasitic diseases in humans. For example, in 2011, a large population of fascioliasis broke out in Binchuan, Yunnan, my country. Due to the lack of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
Inventor 陈汉忠王华俊李义平
Owner GUANGXI UNIV
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