Method for propagating hemerocallis hybridus

Abstract

The invention discloses a method for propagating hemerocallis hybridus. The method for propagating the hemerocallis hybridus comprises the following steps: (1) selecting an explant and sterilizing; (2) inoculating the sterilized explant onto a bud induction medium, and carrying out bud induction culture in a culture room; (3) transferring a differentiated explant onto a subculture medium and carrying out subculture, so as to obtain a differentiated seedling; (4) inoculating the differentiated seedling onto a rooting culture medium and carrying out rooting culture; (5) hardening the differentiated seedling, moving the differentiated seedling out of the culture room, and transplanting, wherein the hemerocallis hybridus is a diploid or multiploid. The method for propagating the hemerocallis hybridus is obviously better than other rapid plant tissue culture propagation methods in the aspects of sterilization time, sterilization effect, operation method, regeneration efficiency, production cost and the like, so that an established large-scale commodity production system is stable and the large-scale rapid propagation production can be realized; the method for propagating the hemerocallis hybridus is mainly applied to rapid propagation of new hemerocallis hybridus varieties and is an effective approach for enabling the new hemerocallis hybridus varieties to be rapidly put into the market, so that the current situation that new varieties are pushed over-slowly in China can be changed.

Description

technical field [0001] The invention relates to a propagation method of Hemerocallis grandiflorum, in particular to a method for propagating Hemerocallis grandiflorum by tissue culture technology. Background technique [0002] Hemerocallis hybridus is rich in germplasm resources and has a wide range of functions. It is "viewed as a flower, eaten as a vegetable, and used as a medicine". The market demand for it is increasing, especially as an ornamental plant. It has a wide variety, beautiful flower shapes, emerald green leaves, rich flower colors, and a long flowering period. It is an excellent garden green space flower that integrates foliage and flower viewing, and is generally loved by people all over the world. Because of its characteristics of easy reproduction, extensive management, strong adaptability and resistance, it meets the requirements of my country's construction of economical gardens. In garden planting, daylilies can be planted in clusters, rows, or in flowe...

AI Technical Summary

Problems solved by technology

The above methods can meet the production needs, but for the excellent new varieties bred in Hemerocallis grandiflora hybrid breeding, if they are to be popularized in a large area, the annual reproduction coefficient of division propagation is 5-10. It takes at least 6-8 years for the new breeds bred to reach a certain scale.

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