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Method of extracting protein and dietary fibers from peony seeds or peony seed meal

A technology of dietary fiber and peony seed meal, which is applied in protein food ingredients, protein composition of vegetable seeds, food preparation, etc., can solve problems such as high temperature unfavorable oil quality, unreported process flow, adverse effects on oil quality, etc. , to achieve the effect of excellent quality, efficient and simple method, and low equipment requirements

Active Publication Date: 2014-01-08
TONGLING RUIPU PEONY IND DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent literature patent number 201210270846.8 (publication number CN 102746940 A) introduces a method for extracting peony seed oil with water. The extraction kettle involved in the inventive method has a pressure of 5-30Mpa and a temperature of 100-300°C, which consumes a lot of energy. And higher temperature is not conducive to maintaining good quality of oil
Chinese patent literature patent number 200910310142.7 (publication number CN 102071096 A) and Chinese patent literature patent number 200910310143.1 (publication number CN 102071097 A) both introduce a method for preparing peony seed oil. The obtained oil needs to go through a series of steps such as filtration, hydration, decolorization, re-filtration, and deodorization. The steps are cumbersome, and a certain amount of alkali needs to be added in the hydration process. quality is adversely affected
Chinese patent literature patent number 20101098582.0 (publication number CN 102277229 A) introduces a processing method of peony seed oil, which requires roasting peony seeds, and the oil yield is not high
[0006] Chinese patent literature patent number 201310077170.5 (publication number CN 103113977 A) introduces a method for synchronously preparing peanut oil and peanut peptide by aqueous enzymatic method. The wall is broken, and the prepared peanut peptide is obtained by directly spray-drying the aqueous phase, which has the problem of low purity of the product
[0007] The present invention takes the three-step enzymatic hydrolysis as the core, and simultaneously obtains peony seed oil, peony seed protein and peony seed fiber in combination with processes such as demulsification, protein precipitation, and slag washing. This technological process has not been reported, and the enzymatic method is applied to peony seeds Oil extraction has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The raw materials are crushed and passed through a 40-mesh sieve. Take 50g of seed powder, add 250ml of water, and mix into a slurry for later use. Adjust the pH of the slurry to 4.5 with 1mol / L hydrochloric acid, and add 15mL of cellulose-pectin complex enzyme (Denmark Novozymes Celluclast 1.5L: polygalacturonase (pectinase) Pectinex Ultra SP-L Compounded with 2:1, enzyme activity 1.2*10 5 U / g), stirred and extracted at 50°C for 2h to obtain enzymatic hydrolysate A. Adjust the pH of enzymatic hydrolysate A with 1mol / L sodium hydroxide to 7 and add 6mL α-medium temperature amylase (Novozymes medium temperature amylase BAN 480, enzyme Live 4*10 4 U / g), stirred and extracted at 70°C for 0.5h to obtain enzymatic hydrolysate B. Adjust the pH of enzymatic hydrolysate B to 8.5 with 1mol / L sodium hydroxide, and add 1g alkaline protease (Novozymes Alcalase 2.4L, Enzyme Live 2.5*10 5 U / g), stirring and extracting at 60°C for 2h to obtain the final enzymatic hydrolysate. Centr...

Embodiment 2

[0031] The raw materials are crushed and passed through a 40-mesh sieve. Take 1000g of seed powder, add 4L of water, and mix into a slurry for later use. Adjust the pH of the slurry to 4.5 with 1mol / L hydrochloric acid, and add 30g of cellulose-pectin complex enzyme (commercially available domestic cellulase: pectinase compounded at 1:1, enzyme activity 5*10 4 U / g), stirred and extracted at 50°C for 2.5 hours to obtain enzymatic hydrolysate A. Adjust the pH of enzymatic hydrolysate A with 1mol / L sodium hydroxide to 7 and add 20g α-medium temperature amylase (commercially available domestic α-medium temperature amylase, enzyme Live 1*10 4 U / g), stirred and extracted at 70°C for 1 hour to obtain enzymatic hydrolysis solution B. Adjust the pH of enzymatic hydrolysis solution B to 8.5 with 1mol / L sodium hydroxide, and add 50g alkaline protease (commercially available domestic alkaline protease, enzyme activity 1 *10 5 U / g), stirring and extracting at 60°C for 2h to obtain the fina...

Embodiment 3

[0038] The raw materials are crushed and passed through a 40-mesh sieve. Take 500g of seed powder, add 2.5L of water, and mix into a slurry for later use. Adjust the pH of the slurry to 4.5 with 1mol / L hydrochloric acid, and add 45g of cellulose-pectin complex enzyme (commercially available domestic cellulase: pectinase compounded at 1:1, enzyme activity 2*10 4 U / g), stirred and extracted at 50°C for 2.5h to obtain enzymatic hydrolysate A. Adjust the pH of enzymatic hydrolysate A with 1mol / L sodium hydroxide to 7 and add 8g α-medium temperature amylase (commercially available domestic α-medium temperature amylase, enzyme Live 1*10 4 U / g), stirred and extracted at 70°C for 1 hour to obtain enzymatic hydrolysate B. Adjust the pH of enzymatic hydrolysate B to 8.5 with 1mol / L sodium hydroxide, and add 35g alkaline protease (commercially available domestic alkaline protease, enzyme activity 5 *10 4 U / g), stirring and extracting at 60°C for 2h to obtain the final enzymatic hydrolys...

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Abstract

The invention discloses a method of extracting protein and dietary fibers from peony seeds or peony seed meal. The method includes the steps of 1, pretreating; 2, performing enzymolysis; 3, centrifuging; 4, demulsifying; 5, recovering protein; 6, washing residue. After the pretreatment of raw materials, final enzymatic hydrolysate is obtained by means of three-step enzymolysis; after centrifuging of the final enzymatic hydrolysate, free oil, an emulsion layer, protein hydrolysate and residue are obtained. The free oil is peony seed oil. After isoelectric precipitation, the protein hydrolysate is refrigerated and dried into peony seed protein powder. The residue is washed and dried so that peony seed dietary fibers are obtained. A certain amount of oil can also be recovered in the demulsifying and residue washing steps. The method has the advantages that peony seeds are utilized comprehensively, the raw materials need not be dried, equipment are less demanded, operation is simple, no solvent residue occurs, no environmental pollution occurs, oil yield is high, oil quality is good, protein yield is high, and swelling property of the dietary fibers is good.

Description

Technical field [0001] The invention relates to a method for extracting edible protein and dietary fiber from peony seeds or peony seed meal, and belongs to the field of agricultural product processing and comprehensive utilization of by-products. Background technique [0002] Peony is a shrub of the genus Paeonia in the Ranunculaceae family. It is widely planted in all parts of my country. The annual output of peony seeds is tens of thousands of tons. The resources are rich and have great development potential. In recent years, the development and utilization of peony seeds has received extensive attention. Numerous studies have shown that peony seeds have high nutritional value, with an oil content of about 29-34% and a protein content of about 18-22%. On March 22, 2011, the Ministry of Health approved peony seeds as a new resource food-"Announcement of the Ministry of Health on Approving Acer Truncatum Seed Oil and Peony Seed Oil as New Resource Foods" (No. 9 of 2011). [0003]...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/04A23J1/14A23L1/308A23L33/22
Inventor 彭瑶瑶聂荣京赵孝庆王洪新高传忠苏建辉
Owner TONGLING RUIPU PEONY IND DEV
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