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Novel use of S100 calcium binding protein A11 for preparation of drug for relaxing airway smooth muscle

A technology for airway smooth muscle and calcium binding protein, which is applied in the field of asthma treatment drugs to achieve good clinical application prospects, safe use and the effect of relaxing airway smooth muscle

Active Publication Date: 2014-01-15
SHANGHAI RES INST OF ACUPUNCTURE & MERIDIANS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our study found that S100A11 has the function of relaxing airway smooth muscle, but there is no report about this function at home and abroad. This new function may provide a new method for the prevention and treatment of asthma

Method used

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  • Novel use of S100 calcium binding protein A11 for preparation of drug for relaxing airway smooth muscle
  • Novel use of S100 calcium binding protein A11 for preparation of drug for relaxing airway smooth muscle
  • Novel use of S100 calcium binding protein A11 for preparation of drug for relaxing airway smooth muscle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 isolated tissue experiment

[0045] 1. Experimental materials

[0046] 1.1 Experimental objects

[0047] Spiral strips of rat bronchial smooth muscle

[0048] Preparation of bronchial spiral strips: after rats were anesthetized with 1% pentobarbital sodium (50 mg / kg), the trachea was separated, and spiral strips with a width of about 5 mm and a length of 15 mm were made according to the method recorded in the literature, and hung in the Gram-Heinz solution In the bath, the gas of 95% O2 + 5% CO2 is passed to keep Po2>53.3kPa in the solution, and maintain a constant temperature of 37°C. The tracheal coil was connected to a tension transducer, and the tension changes were recorded with a physiological recorder. Before the experiment, the tracheal strips were balanced for 1 hour under the initial tension of 1.5g, and the tracheal strips were pre-shrunk with Ach (8mg / ml) 2 to 3 times to achieve the maximum contraction. The experiment was performed after wash...

Embodiment 2

[0070] Embodiment 2 animal experiments

[0071] 1. Experimental materials

[0072] 1.1 Experimental objects

[0073] SD rats, male, 4 weeks old, weighing 100-120 g.

[0074] 1.2 Experimental reagents

[0075] 1) Preparation of S100A11 protein reagent: use pH 7.4 PBS buffer solution to prepare S100A11 reagent with a concentration of 1000ng / ml for use.

[0076] 2) Preparation of OVA reagent: intraperitoneal injection of sensitization solution containing 1mg OVA and 10mgAl(OH) 3 1ml of normal saline was injected intraperitoneally into each rat. The excitation solution was 0.5% OVA, and normal saline was used as the solvent, and the stimulation was injected into the external jugular vein at a dose of 5 mg / kg.

[0077] 3) Other reagents are prepared with deionized water for analytically pure drugs.

[0078] 2. Experimental method

[0079] 2.1 Preparation of asthma model

[0080] SD rats were sensitized by intraperitoneal injection of ovalbumin (OVA), and the SD rats were ch...

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Abstract

The invention relates to the field of a protein drug, and particularly discloses novel use of S100 calcium binding protein A11 for preparation of a drug for relaxing airway smooth muscle, and a drug preparation containing the protein. The invention discloses a novel function of the S100 calcium binding protein A11 for relaxing the airway smooth muscle. The airway smooth muscle can be obviously relaxed by the S100A11; the airway resistance of an asthma model rat is reduced, so as to play a role of treatment of asthma. The S100 calcium binding protein A11 can be applied to treatment of an asthma disease and preparation of a drug for treating asthma, and is applied to the quality of the asthma disease in a form of an injection or spray. Therefore, the S100 calcium binding protein A11 has the advantages of being safe, effective and stable to use and the like.

Description

technical field [0001] The invention relates to the field of protein drugs, and specifically discloses the use of an S100 calcium-binding protein A11 for preparing an asthma treatment drug and the asthma treatment drug containing the recombinant protein. Background technique [0002] S100 calcium binding protein A11 (S100 calcium binding protein A11, S100A11) is one of the important members of the S100 family, also known as S100 calcium binding protein C (S100C), Calgizzarin, lymph node metastasis Gene protein 70 (Metastatic lymph node gene 70protein, MLN70), etc. [0003] S100A11 was first discovered in chicken gizzard smooth muscle cells in 1989. Its monomer contains two EF-hand motifs at the N-terminal and C-terminal, and two monomers of the N-terminal EF-hand (N-terminal EF-hand) To interact in a parallel fashion to form the S100A11 dimer, the Ca 2+ Has a high affinity. in ca 2+ In the free state, the S100A11 protein forms a compact spherical structure with conserved...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P11/00A61P11/06
Inventor 杨永清刘晓燕魏颖徐玉东王宇尹磊淼
Owner SHANGHAI RES INST OF ACUPUNCTURE & MERIDIANS
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