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Bacteria for controlling cotton verticillium wilt and its preparation method and their application

A technology of bacterial agent and bacterial count, which is applied in the field of bacterial agent and its preparation, can solve the problems of unsatisfactory effects of cotton verticillium wilt and achieve the effect of broad application prospects

Active Publication Date: 2016-03-30
中农科合肥食品营养与健康创新研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the unsatisfactory effect of preventing and treating cotton verticillium wilt in existing biological agents, and to provide a bacterial agent capable of effectively preventing and controlling cotton verticillium wilt, its preparation method and their application

Method used

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Examples

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Effect test

preparation example Construction

[0013] The present invention also provides a preparation method of the bacterial agent, wherein the method comprises: Bacillus subtilis, Bacillus pumilus, Bacillus cereus, Streptomyces viridogriseus and Pseudomonas fluorescens was inoculated in the medium for cultivation.

[0014] According to the present invention, the cultivation method includes: respectively cultivating Bacillus subtilis, Bacillus pumilus, Bacillus cereus, Streptomyces viridogriseus and Pseudomonas fluorescens (Pseudomonas fluorescens), and the microorganisms cultured separately were mixed in proportion. Preferably, by cultivating in separate culture systems and mixing after culturing, the total number of viable bacteria per gram of bacterial agent obtained is 1-5×10 10 indivual.

[0015] In a preferred embodiment, by cultivating in separate culture systems and mixing in proportion after culturing, the mixing conditions are not particularly limited, as long as the obtained microbial agent can meet the fol...

Embodiment 1

[0025] (1) Inoculate 100 parts by weight of potato sucrose medium (chop 200g of peeled potatoes, boil them to obtain potato juice, add 20g of glucose or sucrose, add 20g of agar, add water to 1000mL, and sterilize at 121°C) for 5 Parts by weight of Bacillus subtilis (ACCC01659), cultured at 30°C, samples were taken during the cultivation process and observed by direct microscope counting until the number of viable Bacillus subtilis was 1×10 10 per gram of culture medium.

[0026] (2) Inoculate 100 parts by weight of potato sucrose medium (chop 200g of peeled potatoes, boil them to obtain potato juice, add 20g of glucose or sucrose, add 20g of agar, add water to 1000mL, and sterilize at 121°C). Parts by weight of Bacillus pumilus (ACCC01171), cultured at 30°C, samples were taken during the cultivation and observed by direct counting under a microscope until the number of viable Bacillus pumilus was 1×10 10 per gram of culture medium.

[0027] (3) Inoculate 100 parts by weight...

Embodiment 2

[0032] (1) Inoculate 100 parts by weight of potato sucrose medium (chop 200g of peeled potatoes, boil them to obtain potato juice, add 20g of glucose or sucrose, add 15g of agar, add water to 1000mL, and sterilize at 121°C) with 4 Parts by weight of Bacillus subtilis (ACCC01659), cultured at 30°C, samples were taken during the cultivation and observed by direct counting under a microscope until the number of viable Bacillus subtilis was 0.8×10 10 per gram of culture medium.

[0033] (2) Inoculate 100 parts by weight of potato sucrose medium (chop 200g of peeled potatoes, boil them to obtain potato juice, add 20g of glucose or sucrose, add 15g of agar, add water to 1000mL, and sterilize at 121°C). Parts by weight of Bacillus pumilus (ACCC01171), cultured at 30°C, samples were taken during the cultivation and observed by direct counting under a microscope until the number of viable Bacillus pumilus was 0.8×10 10 per gram of culture medium.

[0034] (3) Inoculate 100 parts by w...

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Abstract

The invention provides a microbial inoculum capable of preventing and treating the cotton verticillium wilt. The microbial inoculum comprises a culture medium and thallus, wherein the thallus includes the Bacillus subtilis, the Bacillus pumilus, the Bacillus cereus, the Streptomyces viridogriseus and the Pseudomonas fluorescens. The microbial inoculum can effectively prevent and treat the cotton verticillium wilt, the risk of explosion of the cotton verticillium wilt is lowered, the income of cotton farmers is improved, and the microbial inoculum has wide application prospects. The invention further provides a preparation method of the microbial inoculum and application of the microbial inoculum for preventing and treating the cotton verticillium wilt.

Description

technical field [0001] The present invention relates to a fungal agent and its preparation method and application; more specifically, it relates to a fungal agent for preventing and treating cotton verticillium wilt, a preparation method thereof and their application in preventing and treating cotton verticillium wilt. Background technique [0002] Cotton verticillium wilt is a fungal disease caused by Verticillium dahliae, and it is a very harmful vascular system disease. Verticillium dahliae caused by Verticillium dahliae is a disease of almost all cotton producing countries. Major limiting factor in cotton production. Because there are few cotton varieties with high resistance to Verticillium wilt, and the understanding of the pathogenic mechanism of Verticillium wilt and the genetic mode of cotton resistance to Verticillium wilt is still relatively small, therefore, the current control of cotton Verticillium wilt is still based on biological control. Mainly, but the con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125C12R1/07C12R1/085C12R1/39C12R1/465
Inventor 戴小枫马雪峰陈捷胤李蕾孔志强包郁明肖红利徐明桂月晶祁伟彦王新艳
Owner 中农科合肥食品营养与健康创新研究院有限公司
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