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A kind of compound bacteria agent for preventing and treating Verticillium wilt of tomato and its preparation method and application

A compound bacterial agent and Verticillium wilt technology, applied in the field of agricultural biology, can solve the problems of bacterial resistance, polluted soil, and low feasibility

Active Publication Date: 2018-08-14
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Crop rotation is not feasible for facility agriculture, and although the application of chemical agents can effectively prevent the occurrence of tomato verticillium wilt, it is highly toxic and seriously pollutes the soil. Long-term use will also cause pathogenic bacteria to develop drug resistance and seriously endanger the ecological balance.
After inquiry, there is currently no report on the use of compound Bacillus microbial agents to control tomato Verticillium wilt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A compound microbial agent for preventing and treating Verticillium wilt of tomato, said composite bacterial agent is composed of the fermentation product of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812, the fermentation product of Bacillus subtilis (Bacillus subtilis) MES810 and jelly-like bacillus (Bacillus mucilaginosus) ) The fermentation product of MES803 is mixed according to the volume ratio of 1:3:4;

[0053] The Bacillus subtilis (Bacillus subtilis) MES810 is preserved in the China Common Microorganisms Collection Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is August 10, 2017, and the preservation number is For CGMCCNo.14514;

[0054] The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 is preserved in the China Common Microorganisms Collection and Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang D...

Embodiment 2

[0088] A compound microbial agent for preventing and treating Verticillium wilt of tomato, said composite bacterial agent is composed of the fermentation product of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812, the fermentation product of Bacillus subtilis (Bacillus subtilis) MES810 and jelly-like bacillus (Bacillus mucilaginosus) ) The fermentation product of MES803 is mixed according to the volume ratio of 1:2:3;

[0089] The Bacillus subtilis (Bacillus subtilis) MES810 is preserved in the China Common Microorganisms Collection Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is August 10, 2017, and the preservation number is For CGMCCNo.14514;

[0090] The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 is preserved in the China Common Microorganisms Collection and Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang D...

Embodiment 3

[0124] A kind of composite bacterial agent of preventing and treating Verticillium wilt of tomato, described composite bacterial agent is made of the fermentation product of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812, the fermentation product of Bacillus subtilis (Bacillus subtilis) MES810 and jelly-like bacillus (Bacillus mucilaginosus) ) The fermentation product of MES803 is mixed according to the volume ratio of 1:2:4;

[0125] The Bacillus subtilis (Bacillus subtilis) MES810 is preserved in the China Common Microorganisms Collection Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is August 10, 2017, and the preservation number is For CGMCCNo.14514;

[0126] The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 is preserved in the China Common Microorganisms Collection and Management Center, and the preservation address is No. 3, No. 1, Beichen West Road, C...

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Abstract

The invention relates to a compound bacterial agent for preventing and treating Verticillium wilt of tomato and its preparation method and application. The composite bacterial agent is composed of the fermentation product of Bacillus amyloliquefaciens MES812, the fermentation product of Bacillus subtilis MES810 and the fermentation product of Bacillus jellylike MES803 The products are mixed according to the volume ratio of 1:1-3:1-5. The preparation method of the bacterial species in the composite bacterial agent is as follows: activation of the bacterial species; preparation of the primary seed liquid; preparation of the secondary seed liquid: fermenting in a fermenter, and discharging to obtain a fermented product. The present invention utilizes the large-area reproduction of the main microorganisms in the composite bacterial agent in the soil to antagonize the growth of pathogenic bacteria, effectively inhibit or kill harmful microorganisms in the soil, and provide a powerful environment for the reproduction and development of beneficial microorganisms. Continuously form the occurrence of effective prevention and treatment of tomato Verticillium wilt. At the same time, the soil is improved, soil fertility is improved, and tomato yield and quality are increased.

Description

technical field [0001] The invention belongs to the field of agricultural biology, and relates to a compound microbial agent for preventing and treating tomato verticillium wilt, a preparation method and application thereof. Background technique [0002] Tomato is a herbaceous plant in the Solanaceae tomato genus, which has the value of food and medicine. In recent years, with the continuous expansion of the tomato cultivation area, coupled with successive plantings, it is difficult to fall stubble, which has aggravated the disease of tomato Verticillium wilt year by year, resulting in a significant reduction in yield in diseased fields, and seriously reduced the quality of tomato fruits and made them lose their commerciality. Verticillium wilt has become one of the most important diseases in facility tomato, and it is a bottleneck restricting tomato production and commercialization. Verticillium wilt is a soil-borne vascular disease caused by Verticillium dahliaKieb. It mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10A01N63/02A01P3/00C12R1/07C12R1/125
CPCA01N63/10B09C1/10C12N1/20C12N1/205C12R2001/07C12R2001/125
Inventor 孙海远赵钢勇顾欣燕刘亚利
Owner 天津开发区坤禾生物技术有限公司
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