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A kind of Bacillus fermentation medium, fermentation method and application

A fermentation medium and bacillus technology, applied in the field of Bacillus fermentation medium, can solve the problem of reducing the number of effective viable bacteria, and achieve the effects of improving the use effect, avoiding the loss of microorganisms, and promoting growth.

Inactive Publication Date: 2021-05-04
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above-mentioned technical problems, a kind of bacillus fermentation medium and fermentation method provided by the present invention retain the bacillus fermentation broth, which reduces the problem of the reduction of the number of effective viable bacteria in the existing bacillus production process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A bacillus (Paenibacillus polymyxa) fermentation medium is made of the following components in mass percentage: 8% nitrogen source, 15% sucrose, 0.1% additive, 0.1% defoamer, and the balance is water; Described nitrogen source is the mixture of organic nitrogen source and inorganic nitrogen source; Described organic nitrogen source is the soybean meal powder that has crossed 60 mesh sieves; Described inorganic nitrogen source is ammonium nitrate; And the organic nitrogen source and inorganic nitrogen source in nitrogen source The mass ratio is 1000:1; the additive is indole acetic acid; the defoamer is a commercially available dimethoxysilane defoamer.

[0035] Utilize the method for carrying out fermentation of bacillus fermentation medium, comprise the following steps:

[0036] Step 1, weighing each component according to the following mass percentage: nitrogen source 8%, sucrose 15%, additive 0.1%, defoamer 0.1%, and the balance is water;

[0037] Step 2, mixing mat...

Embodiment 2

[0042]A bacillus (Bacillus subtilis) fermentation medium is made of the following components in mass percentage: nitrogen source 15%, sucrose 8%, additive 0.5%, defoamer 0.2%, and the balance is water; the nitrogen The source is a mixture of an organic nitrogen source and an inorganic nitrogen source; the organic nitrogen source is soybean flour that has passed a 60 mesh sieve; the inorganic nitrogen source is ammonium sulfate; and the mass ratio of the organic nitrogen source to the inorganic nitrogen source in the nitrogen source 500:1; the additive is a mixture of indole acetic acid and peptone in a mass ratio of 1:10; the defoamer is a commercially available THIX-299 polyether modified silicon defoamer.

[0043] Utilize the method for carrying out fermentation of bacillus fermentation medium, comprise the following steps:

[0044] Step 1, each component is weighed according to the following mass percentage: nitrogen source 15%, sucrose 8%, additive 0.5%, defoamer 0.2%, and...

Embodiment 3

[0050] A bacillus (Bacillus licheniformis) fermentation medium is made of the following components in mass percentage: nitrogen source 12%, sucrose 10%, additive 0.3%, defoamer 0.15%, and the balance is water; the nitrogen The source is a mixture of an organic nitrogen source and an inorganic nitrogen source; the organic nitrogen source is soybean milk (soybean milk is made by mixing soybeans and water in a mass ratio of 1:10, then pulverizing and boiling for 20 minutes in a soymilk machine); the inorganic nitrogen Source is ammonium sulfate; And the mass ratio of organic nitrogen source and inorganic nitrogen source in nitrogen source is 750:1; Described additive is yeast extract, corn steep steep powder, peptone, lysine, indole acetic acid according to 3:3: 3:1:1 mass ratio mixed mixture; the defoamer is a commercially available THIX-299 polyether modified silicon defoamer.

[0051] Utilize the method for carrying out fermentation of bacillus fermentation medium, comprise th...

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PUM

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Abstract

The invention belongs to the technical field of microorganism culture substrates, and specifically discloses a bacillus fermentation medium, a fermentation method and an application. It is made of the following components in mass percentage: nitrogen source 8-15%, sucrose 8-15%, additive 0.1-0.5%, defoamer 0.1-0.2%, and the balance is water; the nitrogen source is an organic nitrogen source A mixture with an inorganic nitrogen source; the additive is one or more of yeast extract, corn steep liquor dry powder, peptone, lysine, and indole acetic acid; the defoamer is Tween 80 or silicon-based defoamer agent. The present invention adopts special culture medium materials and proportions, without prior activation of Bacillus seeds for cultivation and strict aseptic preservation of the seeds, which is convenient for on-site operation; the number of effective colonies in the fermentation liquid is high, and the prepared fermentation liquid can be directly It is in the mixed state of bacillus vegetative body and spores, and the use effect is significantly improved, avoiding the loss of microorganisms caused by the secondary processing of the fermentation broth.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture substrates, and in particular relates to a bacillus fermentation medium, a fermentation method and an application. Background technique [0002] Extensive use of chemical pesticides has resulted in pesticide residues in food and environmental pollution, which has seriously threatened human health. In addition, the use of a large number of chemical pesticides can easily cause plant pathogenic bacteria and insects to develop resistance, thereby reducing the control effect. Biological pesticides are considered to be ideal substitutes for chemical pesticides due to their harmless, environmentally friendly, and non-resistance characteristics. [0003] Bacillus is a beneficial microorganism on the leaf surface and rhizosphere of plants, and it is also a common endophytic bacteria in plants. Bacillus can control plant diseases and insect pests and promote plant growth through mechanisms s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07C12R1/10C12R1/125
CPCC12N1/20
Inventor 刘坤王健孙忠科梁明现
Owner ZHOUKOU NORMAL UNIV
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