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Plant fungicide derived from Tripterygium wilfordii and its preparation method and application

A plant fungicide, tripterygium wilfordii technology, applied in the field of plant fungicide and its preparation, can solve the problem that the effect has not yet been reported and the like

Active Publication Date: 2019-05-28
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is used for damp-heat nodules and cancer tumors to accumulate poison. It is used clinically to treat leprosy reactions and rheumatoid arthritis. It has anti-tumor and anti-inflammatory effects. It is a traditional Chinese medicine with high medicinal value, but it is used as a plant The efficacy of fungicides in the control of plant fungal diseases has not been reported

Method used

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  • Plant fungicide derived from Tripterygium wilfordii and its preparation method and application
  • Plant fungicide derived from Tripterygium wilfordii and its preparation method and application
  • Plant fungicide derived from Tripterygium wilfordii and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 Tripterygium wilfordii fungicide

[0021] (1) Dried Tripterygium wilfordii at 50°C to constant weight, then mechanically crushed to 40 mesh;

[0022] (2) Mix the above pulverized Tripterygium wilfordii with water in proportion, the mass volume ratio is 1 / 2, after soaking for 2 hours, heat and boil at 300°C for 30 minutes on a magnetic stirring heater, and filter out the extract after cooling; Add 2 times the volume of water, repeat the above extraction process 2 times, and combine the extracts obtained three times to obtain the crude extract of Tripterygium wilfordii;

[0023] (3) Rotate the crude extract of Tripterygium wilfordii with a rotary evaporator at 60°C, concentrate it to a mass volume equal to the dry weight of Tripterygium wilfordii, add 1wt%, 2wt% or 3wt% of organic silicon surfactants respectively, and obtain tripterygium wilfordii Vine fungicide.

Embodiment 2

[0024] Embodiment 2 Tripterygium wilfordii fungicide inhibits the growth of cotton Verticillium dahliae pathogenic mycelia

[0025] With the fungicide prepared in Example 1, according to the growth rate measurement method recommended by the plant disease research method, the biological activity of the cotton Verticillium dahliae pathogenic bacteria was measured: get the vigorous growth pathogenic bacterial classification at the edge of the 5mm colony with a puncher, put Add the same amount of sterile water to the culture medium as a control on the PDA plate with the drug added, repeat 3 times each time, place it in a constant temperature culture at 25°C, measure the diameter of each colony by the cross method, and take the average value To represent the diameter of the bacterium colony, the following formula is used to obtain the inhibition rate of mycelial growth:

[0026] Inhibition of mycelium growth rate=(control colony diameter-treatment colony diameter) / (control colony d...

Embodiment 3

[0030] Embodiment 3 Tripterygium wilfordii fungicide inhibits germination of cotton Verticillium dahliae pathogenic spores

[0031] With the fungicide obtained in Example 1, according to the spore germination assay method recommended by the plant disease research method, the germination rate of the cotton Verticillium dahliae pathogenic spores is measured: cotton will be cultivated on the potato sucrose agar medium for 7 days with sterile water The spores of the Verticillium dahliae pathogenic bacteria were eluted and shaken well, and the number was maintained at about 100 in the field of view of the low magnification lens (×40) of the microscope by the double dilution method. Using the glass slide hanging drop method, drop 10 μL of the spore suspension on the concave glass slide, and take 10 μL of the diluents of tripterygium wilfordii fungicides with different concentrations and drop them into the spore suspension, so that the final concentration of the tripterygium wilfordii...

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Abstract

The invention relates to the field of biological pesticides, specifically to a botanical fungicide derived from tripterygium wilfordii, and a preparation method and an application thereof. The fungicide comprises 97 wt% to 99 wt% of a tripterygium wilfordii extract and 1 wt% to 3 wt% of an organosilicon surfactant. The fungicide provided by the invention can effectively inhibit pathogen hyph growth and pathogen spore germination of cotton verticillium wilt, so the cotton verticillium wilt is effectively prevented and treated, and a botanical pesticide with high efficiency, low toxicity and low residue is provided for effective prevention and treatment of the cotton verticillium wilt.

Description

technical field [0001] The invention relates to the field of biological pesticides, in particular to a preparation method and application of a plant fungicide derived from tripterygium wilfordii. Background technique [0002] Cotton Verticillium wilt is a soil-borne vascular fungal disease caused by Verticillium dahliae, which seriously affects cotton yield and fiber quality. The area of ​​cotton planted in my country is about 80 million mu every year. Due to the continuous cropping all year round, the accumulation of Verticillium dahliae in the soil, and the lack of disease-resistant varieties and pesticides with good control effects, the control of Verticillium wilt in current production is almost blank. The occurrence and harm of diseases are becoming more and more serious, and become one of the main limiting factors for the development of cotton production. [0003] Botanical pesticides are preparations processed from active ingredients extracted or separated from plant ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/14A01N25/30A01P3/00
CPCA01N25/30A01N65/00A01N65/14A01N2300/00
Inventor 冯自力朱荷琴冯鸿杰李志芳师勇强赵丽红魏锋李云卿周京龙袁媛
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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