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Prunus armeniaca alpha-glucosidase inhibiting peptide as well as preparation method and application of inhibiting peptide

A technology of glucosidase and inhibitory peptide, which is applied in the field of alpha-glucosidase inhibitory peptide and its preparation, and can solve the problems to be improved and the like

Inactive Publication Date: 2014-01-29
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current methods for preparing natural α-glucosidase inhibitors still need to be improved

Method used

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  • Prunus armeniaca alpha-glucosidase inhibiting peptide as well as preparation method and application of inhibiting peptide
  • Prunus armeniaca alpha-glucosidase inhibiting peptide as well as preparation method and application of inhibiting peptide
  • Prunus armeniaca alpha-glucosidase inhibiting peptide as well as preparation method and application of inhibiting peptide

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Experimental program
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Effect test

Embodiment 1

[0068] According to the method for preparing apricot α-glucosidase inhibitory peptide of the present invention, according to the following steps, prepare apricot α-glucosidase inhibitory peptide:

[0069] 1. Protein Extraction

[0070] Dissolve defatted mountain almond meal powder and water at a ratio of 1:14 (g / mL), hydrolyze at pH 9.0 and hydrolysis temperature 37°C for 60 minutes, stop the hydrolysis reaction, centrifuge to take the supernatant, and Acid precipitation was carried out under the condition of pH 4.1, the precipitate was collected by centrifugation, washed with water to neutrality, and then quickly vacuum freeze-dried and stored, and the protein content was determined to be 89.93% by the Coomassie brilliant blue method.

[0071] 2. Proteolysis

[0072] Use alkaline protease Alcalase to hydrolyze the protein obtained above in order to obtain hydrolyzate, wherein the hydrolysis conditions are: substrate concentration (i.e. solid-liquid ratio) 50g / L, enzyme amoun...

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PUM

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Abstract

The invention discloses a prunus armeniaca alpha-glucosidase inhibiting peptide as well as a preparation method and application of the inhibiting peptide, wherein the prunus armeniaca alpha-glucosidase inhibiting peptide has an amino acid sequence represented in SEQ ID NO: 1. The prunus armeniaca alpha-glucosidase inhibiting peptide is quite high in alpha-glucosidase inhibiting activity, and is capable of effectively inhibiting the activity of the alpha-glucosidase, thus effectively reducing blood sugar, and preparing medicines for treating hyperglycemia and diabetes; the prunus armeniaca alpha-glucosidase inhibiting peptide is low in production cost, and has huge practical value and economic benefit.

Description

technical field [0001] The invention relates to the field of health-care functional food and medicine preparation. Specifically, the present invention relates to azalea α-glucosidase inhibitory peptide and its preparation method and application. Background technique [0002] Diabetes has been listed as the third deadly disease, seriously threatening human health. How to treat diabetes has become a key research topic worldwide. At present, the majority of diabetic patients are type Ⅱ diabetes, and there are many kinds of drugs for the treatment of type Ⅱ diabetes, including insulin secretagogues, biguanides, insulin secretion mode regulators and α-glucosidase inhibitors. Among them, artificially synthesized α-glucosidase inhibitors such as acarbose will be accompanied by some side effects in clinical practice, so more natural and safe α-glucosidase inhibitors are developed for the treatment of type Ⅱ diabetes has great significance. [0003] However, the current methods f...

Claims

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Application Information

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IPC IPC(8): C07K5/062C07K5/078C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16C07K1/30A61K38/05A61P3/10A23L1/29A23L33/00
Inventor 王建中顾欣王丰俊崔洁黄昆王文江
Owner BEIJING FORESTRY UNIVERSITY
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