Killing yeast koji species for Xiaoqu and its preparation method
A technology of small koji species and killing yeast, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as burning koji and sour wine, high energy consumption for sterilization, and high requirements for sterilization of koji materials. Effects of improving wine quality and yield, inhibiting the growth of bacteria, and preventing bacteria from contaminating koji
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Embodiment 1
[0028] A preparation method for killing yeast Xiaoqu species, the specific steps are as follows:
[0029] A, the preparation of rhizopus Q303 rice flour culture: earlier in the triangular flask of 500ml, pack 90 grams by weight percentage water content and be the rice flour of 38%, after sterilizing and cooling, insert the slant test tube seed spore suspension of 7ml rhizopus Q303, After shaking well, place it in an incubator at 28-32°C to cultivate for 40 hours, dig out and dry to obtain the rice flour culture of Rhizopus Q303;
[0030] B, the preparation of the mixed grain culture of killing yeast Sa2:
[0031] a. Preparation of improved bean sprout juice culture medium: take 100 g of mung bean sprouts, boil for 20 minutes, filter with gauze after cooling, add distilled water to 1000 mL in the obtained filtrate, add glucose 80 g, dipotassium hydrogen phosphate 1 g, magnesium sulfate 0.3 g, Potassium chloride 0.2g, sodium chloride 1g, peptone 11g, pH natural, mixed and disso...
Embodiment 2
[0035] A preparation method for killing yeast Xiaoqu species, the specific steps are as follows:
[0036]A, the preparation of rhizopus Q303 rice flour culture: earlier in the triangular flask of 750ml, pack 110 grams by weight percentage water content and be the rice flour of 35%, after sterilizing and cooling, insert the slant test tube kind spore suspension of 8ml rhizopus Q303, After shaking well, place it in an incubator at 28-32°C to cultivate for 44 hours, dig out and dry to obtain the rice flour culture of Rhizopus Q303;
[0037] B, the preparation of the mixed grain culture of killing yeast Sa2:
[0038] a. Preparation of improved bean sprout juice culture medium: take 150g of mung bean sprouts, boil for 25 minutes, filter with gauze after cooling, add distilled water to 1000mL in the obtained filtrate, add glucose 100g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.4g, Potassium chloride 0.3g, sodium chloride 1.5g, peptone 13g, pH natural, mixed and dissolv...
Embodiment 3
[0042] A preparation method for killing yeast Xiaoqu species, the specific steps are as follows:
[0043] A, the preparation of rhizopus Q303 rice flour culture: earlier in the triangular flask of 1000ml, pack 130 grams by weight percentage water content and be the rice flour of 32%, after sterilization cooling, insert the slant test tube kind spore suspension of 9ml rhizopus Q303, After shaking well, place it in an incubator at 28-32°C to cultivate for 48 hours, dig out and dry to obtain the rice flour culture of Rhizopus Q303;
[0044] B, the preparation of the mixed grain culture of killing yeast Sa2:
[0045] a. Preparation of improved bean sprout juice culture medium: take 200g of mung bean sprouts, boil for 30 minutes, filter with gauze after cooling, add distilled water to 1000mL in the obtained filtrate, add glucose 120g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, Potassium chloride 0.4g, sodium chloride 2g, peptone 15g, pH natural, mixed and dissolved...
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