Method for preparing composite microbial ecological agent effervescent tablet

A technology of compound microecology and effervescent tablets, which is applied in the direction of medical formula, drug combination, medical preparations containing active ingredients, etc., can solve the inconvenience of storage and transportation for users, it is difficult to ensure accurate measurement of animals, and reduce the number of viable bacteria To achieve the effect of improving feed utilization, increasing convenience and reducing stress

Active Publication Date: 2014-02-05
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this feeding method is simple and convenient, since the probiotics are mostly lactic acid bacteria, their vitality is relatively fragile, and mixing them in feed will significantly reduce the number of viable bacteria
In addition, due to co-feeding with the feed, it is difficult to ensure the accurate measuremen

Method used

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  • Method for preparing composite microbial ecological agent effervescent tablet
  • Method for preparing composite microbial ecological agent effervescent tablet
  • Method for preparing composite microbial ecological agent effervescent tablet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of probiotics

[0034] (1) Preparation of seeds for production

[0035] Culture of lactic acid bacteria

[0036] Preheat the MRS liquid medium to 37°C, inoculate the lactic acid bacteria seed liquid at 2% inoculation amount, and culture it at 37°C for 18-24 hours, harvest the bacterial liquid and store it at -4°C for later use.

[0037] Culture of Bacillus

[0038] Preheat the Bacillus culture medium to 37°C, inoculate the Bacillus seed solution with 1% inoculation amount, and culture it at 37°C for 18-24 hours, harvest the bacteria solution and store it at 4°C for later use.

[0039] Described lactic acid bacteria are: Bifidobacterium bifidum ( Bifidobacterium bifidum ), Lactobacillus ( Lactobacillus lactis ), Lactobacillus Brucella ( Lactobacillus buchneri ), Bifidobacterium longum ( Bifidobacterium longum ) in one or more.

[0040] Described bacillus is: Bacillus licheniformis ( Bacillus licheniformis ), Bacillus coagulans ( ...

Embodiment 2

[0045] Embodiment 2: Preparation of compound Chinese medicine polysaccharide and Chinese medicine flavone

[0046] a. According to 20 parts of Salvia miltiorrhiza, 15 parts of licorice, 20 parts of ginkgo biloba, 20 parts of astragalus, 15 parts of ginseng, 20 parts of Codonopsis pilosula, and 20 parts of wolfberry, weigh each Chinese medicine raw material, chop it, wash it, soak it in cold water overnight, and then add Purified water with 15 times the weight of the raw material, in a water bath to 90°C, and kept at 90°C, boiled for 2 hours, stirring every 10 minutes during the boiling process;

[0047] b. Discard the dregs of the traditional Chinese medicine in a, collect the medicinal liquid, and centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0048] c. The supernatant in b is subjected to alcohol precipitation, the precipitation is the crude Chinese medicine polysaccharide, and the supernatant ...

Embodiment 3

[0054] Embodiment 3: preparation of lyoprotectant

[0055] Each 1000ml of phosphate buffer contains the following components: 40g of Chinese medicine polysaccharides, 30g of Chinese medicine flavonoids, 8g of vitamin combination (vitamin C 1.6g, vitamin E 0.8g, vitamin D3 0.6g, vitamin B1 2.2g, vitamin K 1.6g, vitamin B2 1.2 g), gelatin 5g, sucrose 50g; or each 1000ml of phosphate buffer contains the following components: Chinese medicine polysaccharide 70g, Chinese medicine flavonoids 40g, vitamin combination 7g (vitamin C 1.4g, vitamin E 1.75g, vitamin D3 0.7g, vitamin B1 1.4 g, vitamin K 0.7g, vitamin B2 1.05g), gelatin 6g, sucrose 60g. Described Chinese medicine polysaccharide and Chinese medicine flavonoid are obtained by embodiment 2.

[0056] Preparation method: Accurately weigh each component, dissolve in 1000ml of phosphate buffer solution, filter and sterilize through a 0.22um filter membrane under sterile conditions, and store at 4°C for later use.

[0057] Phosph...

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Abstract

The invention provides a method for preparing a composite microbial ecological agent effervescent tablet, and belongs to the technical field of veterinary biological products. The method comprises the following steps: (1) preparing probiotics; (2) lyophilizing mixed preparation; (3) preparing the effervescent tablet. The composite microbial ecological agent effervescent tablet has the beneficial effects that the transportation requirement for products can be reduced, and using convenience for users can be improved; a microbial ecological agent can be fed while a live vaccine is immunized by drinking water, so that manpower and materials can be reduced, and stress to chickens can be reduced; the immunity of chickens can be strengthened by using the composite microbial ecological agent effervescent tablet, the feed utilization of the chickens can be improved, the laying rate of the chickens can be increased, the ammonia emission of a chicken coop can be reduced, and the living environment of the chickens can be improved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a preparation method of a compound microecological preparation effervescent tablet. Background technique [0002] Probiotics have beneficial effects on humans and animals. Probiotics in probiotics can produce antibacterial substances, compete with harmful bacteria for niches, inhibit the growth and reproduction of pathogenic bacteria, and adjust and restore the balance of intestinal flora. In addition, probiotics can become non-specific immune factors, stimulate host cells through the bacteria themselves or cell wall components, activate the immune system, increase the body's antibody level or macrophage activity, thereby enhancing the body's resistance. The beneficial bacteria in the probiotics multiply in the digestive tract, which can promote the effective synthesis, absorption and utilization of a series of nutrients such as amino acids and...

Claims

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Application Information

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IPC IPC(8): A61K36/815A61K35/74A61K9/46A61P37/04A61K31/715A61K35/742A61K35/744
Inventor 张秀文李阳闫艳丽刘雪王二先盛璐丝
Owner 浙江美保龙生物技术有限公司
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