Integrated multifunctional microfluidic chip

Abstract

The invention relates to a microfluidic chip used for cell capture, cell lysis, DNA (Deoxyribonucleic Acid) concentration and purification and polymerase chain reaction (PCR) amplification and belongs to the field of biological and medical detection. The invention discloses the microfluidic chip which comprises a four-layer chip structure and cover glass used for a sample inlet and is embedded with a chip layer used for cell capture filter membrane, cell lysis and DNA purification and concentration, a chip layer used for PCR amplification and a substrate layer used for being attached to a heating device. The microfluidic chip is small in size, simple in structure, easy to operate, low in cost, stable in performance, high in analysis and detection sensitivity, short in disease diagnosis period and suitable for diagnosis of related diseases.

Description

technical field [0001] The invention relates to an integrated multifunctional microfluidic chip, which is mainly used for the capture of cells in microbial samples, cell lysis, DNA purification and concentration, and PCR amplification to achieve the purpose of detecting microorganisms in samples, and belongs to life science and medical detection field. Background technique [0002] The concept of micro-total analysis system was first proposed in the early 1990s, and in the following ten years, this field has developed into one of the most cutting-edge scientific and technological fields in the world. The micro-total analysis system integrates many processes and steps of biochemical analysis technology in the current modern macroscopic laboratory. The structure of various instruments and instruments in the biochemical analysis technology laboratory is miniaturized, and at the same time, the functions of the various instruments and instruments in the biochemical analysis tech...

AI Technical Summary

Problems solved by technology

Traditional PCR technology has disadvantages such as large thermal inertia, slow heating and cooling, uneven distribution of reaction temperature, large reaction volume, large amount of reagents, and products that must be transferred and separated.

Images