Microscopic three-dimensional reconstruction method

Abstract

A microscopic three-dimensional reconstruction method is provided. The microscopic three-dimensional reconstruction method comprises the following steps that: a liquid lens of which the focal length can be controlled through voltage is additionally mounted in an optical path of a digital microscope; with the liquid lens adopted as a focal length-varying element, a series of focusing is performed in a continuous depth range of an observed object so as to shoot a series of continuous local focus images; height differences between the local focus images can be obtained through the calibration of the liquid lens in advance; three-dimensional information of a measured object is extracted from the series of images through a three-dimensional reconstruction algorithm, such that a three-dimensional model of the object is obtained; and a two-dimensional clear image is obtained through image fusion, and the two-dimensional clear image, adopted as texture, is bonded on the three-dimensional model, and three-dimensional reconstruction of the object is accomplished. With the microscopic three-dimensional reconstruction method adopted, limitation on object observation in a two-dimensional direction because of too small depth of field of a microscopic optical system can be effectively eliminated.

Description

technical field [0001] The invention relates to the field of micro vision in photoelectric detection technology, and more specifically relates to a device and method for obtaining a micro three-dimensional reconstruction model. Background technique [0002] Because the depth of field of the optical microscope is too short, there are limitations in observing the depth information of the object. When the surface of the object is uneven, if the depth of field of the microscope is short, the image of the captured object will be partially blurred. Therefore, the traditional 3D reconstruction Methods such as binocular vision 3D reconstruction and structured light 3D reconstruction cannot be applied to microscopic 3D reconstruction. [0003] Use a precision lifting platform to control the object under observation or the microscope lens to move up and down, and simultaneously take images of the object and record the height corresponding to each image. In the later stage, the 3D reco...

AI Technical Summary

Problems solved by technology

[0003] Use a precision lifting platform to control the object under observation or the microscope lens to move up and down, and simultaneously take images of the object and record the height corresponding to each image. In the later stage, the 3D reconstruction algorithm can be used to recover the 3D appearance information to achieve 3D reconstruction. The method is limited by the accuracy and speed of the precision lifting platform, and the system is relatively complicated

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