Extraction method for astragalus polysacharin

A technology of astragalus polysaccharide and extraction method, which is applied in the field of extracting polysaccharides and astragalus polysaccharides by using low-temperature liquid nitrogen crushing combined with polysorbate-80-assisted plant compound enzymatic hydrolysis, and can solve the problems of unstable properties of polysaccharides, low extraction rate, and high cost. problems, achieve the effects of shortening pretreatment and extraction time, improving nutrition, and facilitating operation

Active Publication Date: 2014-03-05
越好生物科技(广州)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved in the present invention is to provide a new extraction method of astragalus polysaccharides for the defects of low extraction rate, low purity, high cost and unstable properties of polysaccharides in the extr...

Method used

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  • Extraction method for astragalus polysacharin
  • Extraction method for astragalus polysacharin
  • Extraction method for astragalus polysacharin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for extracting astragalus polysaccharides, comprising the steps of:

[0033] Pretreatment: remove impurities such as mud and sand on the roots of Astragalus mongolica;

[0034] S1. the root of Astragalus mongolica is crushed through low-temperature liquid nitrogen; Astragalus powder is obtained after crossing an 80-mesh sieve; in the crushing process of the low-temperature liquid nitrogen, the feed rate of nitrogen gas is 3mL / s, and the feed rate of Radix Astragali is 4g / s; the crushing temperature -5°C;

[0035] S2. Treat 3 parts of astragalus powder, 12 parts of sterile water, 0.3 parts of polysorbate-800, 0.3 parts of cellulase, and 0.1 part of pectinase at 60°C and pH 4.8 for 1.5 hours; Parts are parts by weight;

[0036] S3. Inactivate the enzyme at 100°C for 15 minutes; then extract at 85°C for 2.5 hours; filter; concentrate for 60 minutes at a vacuum of 95kPa and a concentration temperature of 60°C to obtain a concentrated solution; add 95% ethanol 5 t...

Embodiment 2

[0038] A method for extracting astragalus polysaccharides, comprising the steps of:

[0039] Pretreatment: remove impurities such as mud and sand on the roots of Astragalus mongolica;

[0040] S1. the root of Astragalus mongolica is crushed through low-temperature liquid nitrogen; Astragalus powder is obtained after crossing an 80-mesh sieve; in the crushing process of the low-temperature liquid nitrogen, the feed rate of nitrogen gas is 3mL / s, and the feed rate of Radix Astragali is 4g / s; the crushing temperature -5°C;

[0041] S2. Treat 3 parts of astragalus powder, 12 parts of sterile water, 0.3 parts of polysorbate-600, 0.3 parts of cellulase, and 0.1 part of pectinase at 60°C and pH 4.8 for 1.5 hours; Parts are parts by weight;

[0042] S3. Inactivate the enzyme at 100°C for 15 minutes; then extract at 85°C for 2.5 hours; filter; concentrate for 60 minutes at a vacuum of 95kPa and a concentration temperature of 60°C to obtain a concentrated solution; add 95% ethanol 5 t...

Embodiment 3

[0044] A method for extracting astragalus polysaccharides, comprising the steps of:

[0045] Pretreatment: remove impurities such as mud and sand on the roots of Astragalus mongolica;

[0046] S1. the root of Astragalus mongolica is crushed through low-temperature liquid nitrogen; Astragalus powder is obtained after crossing an 80-mesh sieve; in the crushing process of the low-temperature liquid nitrogen, the feed rate of nitrogen gas is 3mL / s, and the feed rate of Radix Astragali is 4g / s; the crushing temperature -5°C;

[0047] S2. Treat 3 parts of astragalus powder, 12 parts of sterile water, 0.3 parts of polysorbate-400, 0.3 parts of cellulase, and 0.1 part of pectinase at 60°C and pH 4.8 for 1.5 hours; Parts are parts by weight;

[0048] S3. Inactivate the enzyme at 100°C for 15 minutes; then extract at 85°C for 2.5 hours; filter; concentrate for 60 minutes at a vacuum of 95kPa and a concentration temperature of 60°C to obtain a concentrated solution; add 95% ethanol 5 tim...

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Abstract

The invention relates to the health care food processing technology field, and concretely relates to an extraction method for astragalus polysacharin. In the method, polysacharin is extracted by utilization of low temperature liquid nitrogen crushing, non-ionic surface active agent auxiliary and plant compound enzyme enzymolysis. The method is advantaged by easily available raw materials, simple technology, and convenient operation and is suitable for large-scale industrial production. On the one side, the method decreases extraction times, raises yield and purity of the polysacharin, and lowers the production cost, on the other side, the method maintains the stabilization of structures and properties of the polysacharin, and raises trophism, safety and acceptability of the astragalus polysacharin greatly. In addition, the added non-ionic surface active agent is preferably polysorbate-80, polysorbate-80 has promotion effects on cellulase and pectase in hydrolytic process, and has solubilizing effects on the polysacharin at the same time. The pretreatment time and the extraction time are shortened, the time cost is lowered, and economic benefits of enterprises are raised.

Description

technical field [0001] The invention relates to the technical field of health food processing, in particular to a method for extracting astragalus polysaccharides, more specifically, a method for extracting polysaccharides by using low-temperature liquid nitrogen pulverization combined with polysorbate-80-assisted plant compound enzymatic hydrolysis. Background technique [0002] Radix Astragalus (Radix Astragalus) is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus, and is an important traditional Chinese medicine for invigorating Qi. Astragalus has the effects of "promoting body fluid and nourishing yang, tonifying yang and blood, tonifying deficiency and damage, and healing kidney failure". Function, anti-cancer and anti-virus and other functions, its medicinal effect is remarkable, it has a long history and is widely used, and it is one of the commonly used Chinese medicinal materials. [0003] Astragalus Polysacharin (APS) is a wat...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 杜冰张嘉怡谢蓝华王超林凤英周洁静
Owner 越好生物科技(广州)股份有限公司
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