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A compound embedding agent immobilization method for photosynthetic bacteria inhibiting algae

A technology of photosynthetic bacteria, immobilization methods, applied in the direction of chemicals for biological control, immobilized on/in organic carriers, herbicides and algicides, etc., capable of solving adhesion water-soluble swelling, unfavorable application, strength Low-level problems, to achieve the effect of increasing chelation, stable quantity, and easy operation

Inactive Publication Date: 2016-08-17
BEIJING UNIV OF TECH
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Problems solved by technology

The preparation process of ammonium alginate immobilized cells is simple, non-adhesive, and has good mass transfer performance, but the strength is low; there are many studies on the combined immobilization method of sodium alginate and polyvinyl alcohol, but there is serious adhesion phenomenon in the preparation process And the water-soluble swelling is serious, which is not conducive to the application

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Preparation of concentrated solution of photosynthetic bacteria: culture photosynthetic bacteria to the logarithmic phase (OD 680 value of 1.5-1.8), the photosynthetic bacteria suspension at 8000-10000r·min -1 Centrifuge at a rotating speed for 8-12 minutes, wash the precipitate with normal saline and then centrifuge, and repeat this 3 times. Make OD with normal saline 680 Concentrate of 2.3-2.6;

[0031] (2) Preparation of embedding agent: After dissolving 7.0-7.5 (g) (optimum amount 7.2g) sodium alginate in 300mL of distilled water at 60-80°C, add 3-3.5 (g) (optimum amount) 3.2g) of zeolite and calcium carbonate, stir and mix evenly to make a mixed embedding agent;

[0032] (3) Preparation of cross-linking agent: Dissolve 12g (optimum amount) of calcium chloride in 600mL (optimum volume) of saturated boric acid solution, and cool in a refrigerator at 4°C for later use;

[0033] (4) Primary cross-linking: After the mixed embedding agent prepared in step (2) is ...

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Abstract

The invention relates to a method for fixing photosynthetic bacteria inhibiting algae with a composite embedding agent, which belongs to the technical field of water treatment and microorganism immobilization. Choose sodium alginate as embedding agent, zeolite and calcium carbonate as additives, calcium chloride saturated boric acid solution as crosslinking agent, immobilization temperature is 4°C, after immobilization for 1h, adjust the pH for secondary crosslinking after 24h The pellets are washed for standby use; meanwhile, the invention discloses its application for inhibiting algae in eutrophic water bodies. The immobilization method proposed by the present invention adapts to the physiological characteristics of photosynthetic bacteria and can make it maintain good physiological activity; the optimal embedding ratio provided makes the density, mechanical strength, water content, mass transfer, cell activity and The expansibility can be maintained at a good level; the production process is simple, the cost is low, and it is easy to be popularized in a large scale; way.

Description

technical field [0001] The invention belongs to the technical field of water treatment and microorganism immobilization, and in particular relates to a composite embedding agent, a method for immobilizing photosynthetic bacteria and its inhibitory effect on common algae in eutrophic water bodies. Background technique [0002] With the improvement of the level of urbanization and industrialization, the shortage of water resources and the aggravation of water pollution, reclaimed water has become a stable second water source for many water-scarce cities. In the utilization of regenerated water, ecological water as a replenishment landscape water body is one of its most important application areas. Due to the special physical and chemical properties of reclaimed water, it is more likely to cause eutrophication in water than natural water during use due to overgrowth of algae. [0003] Common water algae control technologies can be divided into: physical methods, physicochemica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10A01N63/00A01P13/00
Inventor 吴珊任丽艳厉智成
Owner BEIJING UNIV OF TECH
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