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Automated Circulating Tumor Cell Detection

A technology of cells and detection methods, applied in the diagnosis of tumors, the field of living cells

Active Publication Date: 2017-04-12
SIEMENS HEALTHCARE GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To date, none of the size-based selection methods is fully automated

Method used

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  • Automated Circulating Tumor Cell Detection

Examples

Experimental program
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Effect test

Embodiment Construction

[0121] Solids (cells, particles, tissues) that need to be separated from the surrounding medium (sample liquid) are separated from the medium by retaining them on the surface of a filter membrane that is impervious to the solids to be separated Permeable, whereas permeable to the surrounding medium and to solids that contaminate the solids to be separated. For this purpose, a method is provided which makes it possible to determine and vary the flow rate of a gas or liquid through the permeable surface. In medical diagnostics the solids are preferably cellular components and the surrounding medium is preferably whole blood / serum / plasma which may also contain particles and by their nature permeable to the surface (eg leukocytes).

[0122] In particular all reagents necessary for isolation / enrichment and detection, except the sample, are coated on the surface of the membrane by an automated process, eg by a pipetting robot. The sample and reagents can be controllably retained on...

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Abstract

The present invention is in the field of in vitro diagnostics and relates to methods and devices for the detection of circulating or diffuse living cells in body fluids (e.g. blood, urine), or tissue samples mixed with fluid (e.g. bone marrow), comprising the following steps: a ) filtering the liquid sample through a porous membrane adapted to intercept the cells to be tested, such that the cells to be tested rest on at least part of the surface of the membrane, and allowing the sample liquid to pass through the membrane, b) coating the first treatment liquid , which comprises a first reagent for labeling the test cells with a first marker, c) incubating the treatment solution on the membrane for a predetermined period of time, wherein the test cells are labeled, d) Labeled test cells are detected on the surface of the membrane.

Description

technical field [0001] The present invention is in the field of in vitro diagnostics and relates to methods and devices for the detection of circulating or diffuse living cells in bodily fluids (eg blood, urine), or tissue samples mixed with fluids (eg bone marrow). The method of the present invention is particularly useful for the automatic extraction and analysis of circulating tumor cells, and is preferably applied to the diagnosis of tumors. [0002] The invention realizes automatic detection of cells or cell components from peripheral blood or bone marrow through functional testing after the blood or bone marrow is filtered through a special filtering method. The cells are in particular circulating tumor cells (English: circulating tumor cells, CTCs or (plural) CTCs), mesenchymal stem cells or bacteria from peripheral blood or other body fluids, and disseminated tumor cells (English: disseminated tumor cells) from bone marrow , DTC). Background technique [0003] The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/569
CPCG01N33/5091G01N1/30C12Q1/6806
Inventor K.弗雷德里克J.班戈特W.古姆布雷科特K.希尔陶斯基P.波利卡M.斯坦泽尔
Owner SIEMENS HEALTHCARE GMBH