Inspection method for purity of melon seeds

Abstract

The invention discloses an inspection method for the purity of melon seeds, especially an inspection method for the purity of Jinlu No. 3 melon seeds by using SSR molecular markers. The method utilizes a pair of SSR primers, i.e., FLWSSRL and FLWSSRR, for amplification and agarose gel electrophoresis separation of male and female parents of a hybrid melon variety, i.e., the Jinlu No. 3, and the genome DNA of a first-filial generation; characteristic bands of difference between the first-filial generation and the male and female parents are found out; inspection is carried out on seed samples of the first-filial generation of the Jinlu No. 3 through calculation of the purity of to-be-inspected seeds; and inspected purity is compared with purity obtained in field planting identification, and results show that the inspected purity is highly identical to field planting identification purity. The method is applicable to inspection of authenticity of the Jinlu No. 3 and parents thereof and inspection of seed purity.

Description

technical field [0001] The invention belongs to the technical field of seed quality inspection, and relates to a method for inspecting the purity of hybrid melon seeds, in particular to a method for inspecting the purity of melon Jinlu No. 3 seeds by using SSR molecular markers. Background technique [0002] Jinlu No. 3 is a new high-yield and high-quality hybrid melon variety bred by Hefei Fengle Seed Industry Co., Ltd. (see the "Registration Form for Disclosure of Genetic Resources Sources" for details), and has excellent field agronomic characteristics. During the process of hybrid seed production, the female parent needs to be artificially detasselled, and the male parent pollen is given to complete the hybridization process. Incomplete or missing emasculation of the female parent will greatly affect the purity of the seed production. The traditional hybrid purity detection method is planting identification, which depends on the phenotypic differences of varieties, and i...

AI Technical Summary

Problems solved by technology

During the process of hybrid seed production, the female parent needs to be detasselled artificially, and the male parent pollen is given to complete the hybridization process. Incomplete or missing emasculation of the female parent will greatly affect the purity of the seed production.

The traditional hybrid purity detection method is planting identification, which depends on the phenotypic differences of varieties, and is identified through the comparison of the appearance and shape of adult plants. The identification time is long, the cost is high, the difficulty is relatively large, and the accuracy is poor, especially in the field. Familiarity with related varieties and their parents is a complete empirical judgment. The quality of the appraiser is directly related to the purity of the appraisal and the success of the appraisal. Therefore, field appraisal is increasingly unsuitable for the development of the modern seed industry.

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