In situ polymerase chain reaction plate
An enzyme chain reaction and in-situ polymerization technology, applied in bioreactor/fermenter combinations, bioreactors/fermenters for specific purposes, enzymology/microbiology devices, etc., can solve air leakage, inconvenient operation, Unstable results, etc.
Inactive Publication Date: 2014-03-19
苏州东胜兴业科学仪器有限公司
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Problems solved by technology
[0003] In situ gene amplification technology is an effective detection method, which has been widely used for a long time, but requires high technical requirements for its operation.
However, in practice, due to the PCR amplification on the glass slide, the traditional cover glass is used for sealing. problems, resulting in a low success rate of experiments and very unstable results, which seriously restrict the reliability of in-situ detection technology
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[0021] The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.
[0022] Please refer to figure 1 , figure 2 , image 3 and Figure 5 , an embodiment of the present invention provides an in situ polymerase chain reaction plate 100 . The in situ polymerase chain reaction plate 100 includes an upper plate 110 and a lower plate 120 , and the upper plate 110 is provided with a sealing strip 130 . When performing in situ polymerase chain reaction experiments, the lower plate 120 needs to be pretreated first, and then the upper plate 110 and the pretreated lower plate 120 are connected by a sealing strip 130 to form an opening for accommodating the in situ Cavity 140 for PCR reagents 150 . The sealing strip 130...
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The invention provides an in situ polymerase chain reaction plate. The in situ polymerase chain reaction plate comprises an upper plate and a lower plate, wherein a sealing strip is arranged on the upper plate, the upper plate and the lower plate are connected by the sealing strip to form a cavity provided with an opening and used for accommodating a polymerase chain reaction reagent, and the in situ polymerase chain reaction plate is also provided with a sealing cover used for sealing the cavity. When the in situ polymerase chain reaction experiment is performed, the in situ polymerase chain reaction reagent is filled in the cavity and is sealed by using the sealing cover, thus ensuring the effectiveness of sealing in the in situ polymerase chain reaction process. The in situ polymerase chain reaction reagent does not evaporate and reacts completely, and experimental products do not pollute the environment, so that the success rate of the experiment is increased, thus ensuring diagnosis of specific genes and pathogens and reliably applying and popularizing the in situ polymerase chain reaction technology.
Description
technical field [0001] The invention relates to a device for polymerase chain reaction, in particular to an in-situ polymerase chain reaction plate. Background technique [0002] In situ polymerase chain reaction technology successfully combines polymerase chain reaction technology and in situ hybridization technology, which maintains the advantages of the two technologies and makes up for their respective shortcomings. The specimens to be tested for in situ polymerase chain reaction technology are generally tissue slices or cells on glass slides. The tissue slices or cells are first chemically fixed to keep the tissue cells in good condition. Both the cell membrane and the nuclear membrane have a certain degree of permeability. When performing polymerase chain reaction amplification, various components (such as primers, DNA polymerases, nucleotides, etc.) can enter cells and nuclei, using RNA or DNA fixed in cells or nuclei as templates, Amplification is performed in situ...
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IPC IPC(8): C12M1/00
CPCB01L3/502B01L3/508B01L2200/0689
Inventor 刘志华聂尚海申跃华
Owner 苏州东胜兴业科学仪器有限公司