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Camptothecin preparation capable of inhibiting organism endothelial cell differentiation, and application

A technology of endothelial cells and camptothecin, applied in the field of botanical pesticides, can solve problems such as growth and development obstacles of pests

Inactive Publication Date: 2014-04-02
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a large gap in the toxic mechanism of camptothecin at home and abroad. Our research found that camptothecin can cause insect cell apoptosis by changing the redox state of cells, and change the extremely important intermediate metabolites in the body. Hydroquinone, thereby inhibiting cell differentiation and causing growth and development disorders of pests

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the preparation of the present invention is as follows: dry the same amount of philodendron philodendron leaves, seeds, and fruits in the shade in a ventilated place, crush them to 400 mesh with a pulverizer, soak them in tap water for 13 hours, then heat them to 50°C for 34 minutes, and filter them. Carry out solid-liquid separation, collect the filtrate and filter residue respectively, the obtained filtrate is called A liquid, then recycle the filter residue, add appropriate amount of tap water and repeat the above steps to obtain filtrate B, combine the two filtrates, ultrasonically treat for 7 minutes, and then add the filtrate and the filtrate to the filtrate The same amount of methanol was refluxed twice, each time for 65 minutes, and then stood at 25°C for 10 hours, and the precipitate was removed by suction filtration, and methanol was recovered under reduced pressure at 55°C, and the extract was placed in a water bath at a temperature of...

Embodiment 2

[0024] The preparation method of the preparation of the present invention is as follows: dry the leaves, seeds and fruits of the same amount of philodendron in the shade in a ventilated place, crush them to 200 mesh with a pulverizer, then soak them in tap water for 12 hours, then heat them to 75°C and continue to decoct them for 32 minutes, press filter Carry out solid-liquid separation, collect the filtrate and filter residue respectively, the obtained filtrate is called A liquid, then recycle the filter residue, add an appropriate amount of tap water to repeat the above steps, and obtain the filtrate B, combine the two filtrates, ultrasonicate for 6 minutes, and then add the filtrate and the filtrate to the filtrate The same amount of methanol was refluxed and extracted 3 times, each time for 55 min, and then stood at 25°C for 16 hours, and the precipitate was removed by suction filtration, and methanol was recovered under reduced pressure at 55°C, and the extract was concent...

Embodiment 3

[0026] The preparation method of the preparation of the present invention is as follows: dry the leaves, seeds and fruits of the same amount of philodendron in a ventilated place, crush them to 250 mesh with a pulverizer, then soak them in tap water for 11 hours, then heat them to 55°C for 30 minutes, and filter them. Carry out solid-liquid separation, collect the filtrate and filter residue respectively, the obtained filtrate is called A liquid, then recycle the filter residue, add an appropriate amount of tap water to repeat the above steps, and obtain the filtrate B, combine the two filtrates, ultrasonicate for 5 minutes, and then add the filtrate and the filtrate to the filtrate The same amount of methanol was refluxed and extracted 3 times, each time for 60 min, and then stood at 25°C for 12 hours, and the precipitate was removed by suction filtration, and the methanol was recovered under reduced pressure at 55°C, and the extract was concentrated in a water bath at a temper...

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PUM

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Abstract

The invention discloses a camptothecin preparation capable of inhibiting organism endothelial cell differentiation, and an application, and belongs to the technical field of plant source pesticides. The camptothecin preparation is characterized by comprising 0.5-2.3% of camptothecin extract dry powder, 1.2-5.3% of inorganic base, 82-97% of solvent, 0.7-2.1% of synergist and 1-5% of stabilizer. According to the camptothecin preparation capable of inhibiting the organism endothelial cell differentiation, and the application, a substance after a camptothecin extract reacts with the inorganic base serves as an active component, a certain synergistic component and a certain stabilizing component are added, and the obtained preparation participates in a process of inhibiting the organism endothelial cell differentiation, so that growth and development of injurious insects are inhibited; when the camptothecin preparation serves as a pesticide in a field, photodissociation and loss of the active component in an environment can be reduced, the biological activity can be exerted enduringly, and effects of organism endothelial cell differentiation inhibition and field disease and insect damage control are exerted; and the camptothecin preparation is convenient to prepare, low in cost, stable in effect, and less in dosage.

Description

technical field [0001] The invention belongs to the technical field of botanical pesticides, in particular to a camptothecin preparation capable of inhibiting the differentiation of biological endothelial cells and its application. Background technique [0002] Camptothecia acuminata Decaisne is an ancient and excellent insecticidal plant, and it is a unique tree species in my country. It is fast-growing. Generally produced in southern Jiangsu, Zhejiang, Fujian, Jiangxi, Hunan, Hubei, Sichuan, Guizhou, Guangxi, Guangdong, Yunnan and other provinces, it is more common in the Chengdu Plain in western Sichuan and southeastern Jiangxi. More than 30 compounds have been found in the roots, stems, leaves and seeds of camptothecin, including more than ten camptothecins and other alkaloids, including camptothecin, camptothecin, hydroxycamptothecin, methylcamptothecin Alkali, etc., among which camptothecin has the highest content and is the main active substance. These alkaloids hav...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P5/00A01P7/04
Inventor 张立钦童森淼陈安良胡加付马建义王勇军毛胜凤刘洪波张绍勇
Owner ZHEJIANG FORESTRY UNIVERSITY
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