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Application of clooxine in preparation of medicine for treating or preventing influenza virus infection

A technology for preventing influenza and virus infection, applied in the field of medicine, can solve problems such as no reports of chloroxacin being anti-influenza virus, and achieve the effect of broad-spectrum anti-influenza virus activity and good safety

Inactive Publication Date: 2016-01-06
武汉威立得生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report on the anti-influenza virus of clooxine

Method used

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  • Application of clooxine in preparation of medicine for treating or preventing influenza virus infection
  • Application of clooxine in preparation of medicine for treating or preventing influenza virus infection
  • Application of clooxine in preparation of medicine for treating or preventing influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Evaluation of clooxine anti-influenza virus activity

[0032] 1. Experimental Materials

[0033] 1.1 Cells, viruses and drugs

[0034] MDCK cells were purchased from American Type Culture Collection (ATCC); virus strain: A / PuertoRico / 8 / 34 (H1N1); drug: chloroxine was purchased from Sigma.

[0035] 1.2 Experimental Instruments

[0036] Multilabel microplate reader (PerkinElmer).

[0037] 2. Experimental methods and results

[0038] 2.1 Cell culture: 37°C, 5% CO 2 cultured in a humidified incubator. A DMEM medium containing 10% FBS, 100 U / mL of penicillin and streptomycin was used. Cells were subcultured after reaching 90% confluence, and the subculture ratio was 1 / 3–1 / 4.

[0039] 2.2 Virus culture: Take SPF chicken embryos aged 9 to 11 days, check them with an egg tester before inoculating the virus, mark them at a position far away from the embryos, disinfect and punch holes, and inoculate 2- 4 The virus liquid with hemagglutination titer was sea...

Embodiment 2

[0049] Embodiment 2: Evaluation of broad-spectrum anti-influenza virus effect of clooxine

[0050] In this experiment, clooxine was tested against influenza A subtype H1N1 strain, influenza A virus subtype H3N2 strain, influenza A virus subtype H1N1 amantadine-resistant strain, influenza A virus subtype H6N6 strain, influenza A virus Antiviral activity of virus subtype H7N8 strain, influenza A virus subtype H9N2 strain and influenza B virus.

[0051] 1. The virus strains used in the experiment include: A / Human / Hubei / 1 / 2009(H1N1), A / human / Hubei / 3 / 2005(H3N2), A / human / WSN / 33 / (H1N1, S31N), A / Duck / Hubei / 5 / 2010(H6N6), A / Duck / Hubei / 216 / 1983(H7N8), A / Chicken / Jiangsu / 1 / 2005(H9N2), B / human / Hubei / 1 / 2007.

[0052] 2. Divide MDCK cells into 1.5×10 4 Cells / well were seeded in 96-well cell culture plates, cultured in a 37°C cell culture incubator for 14-18 hours, and the cells were grown into a single layer before use. Discard the medium in the well plate, wash twice with PBS, add 100TCI...

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PUM

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Abstract

The invention discloses the application of chlorooxine (Chloroxine) in the preparation of medicaments for preventing or treating influenza virus infection. Firstly, the toxicity of clooxine to MDCK cells was detected, and the results showed that the maximum non-toxic concentration of clooxine was 25.0 μM. Secondly, the antiviral activity of clooxacin was measured in a completely non-toxic concentration range, and the results showed that this small molecule compound had significant antiviral activity, and the antiviral effect was dose-dependent. Finally, the antiviral activity of clooxacin on different types and subtypes of influenza virus was detected, and the results showed that clooxacin could inhibit the replication of all detected influenza virus strains in a dose-dependent manner, indicating that clooxacin had anti-influenza virus activity. broad spectrum. Therefore, the present invention discloses that clooxine is a novel broad-spectrum anti-influenza virus drug, which can be used to prepare drugs for preventing or treating influenza virus infection.

Description

technical field [0001] The invention relates to the application of clooxine in the preparation of medicines for treating or preventing influenza virus infection, and belongs to the technical field of medicine. Background technique [0002] Influenza viruses belong to the family Orthomyxoviridae, the genus Influenzavirus. According to the differences in the antigenic and genetic characteristics of the virion nucleoprotein (NP) and matrix protein (M), influenza viruses are divided into three types: A, B, and C, also known as A, B, and C. The whole genome of influenza A virus consists of eight single-stranded negative-strand RNAs of different sizes, named after segment 1 to segment 8, respectively. The full-length viral genome is about 13.6kb, encoding 10 structural proteins (PB2, PB1, PA, HA, NP, NA, M1, M2, PB1-F2 and NS2 / NEP) and non-structural proteins (NS1). Influenza A viruses can be further divided into 17 H (H1-H17) and 10 N (N1-N10) subtypes according to the differen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/47A61P31/16
Inventor 陈绪林安利伟刘锐
Owner 武汉威立得生物医药有限公司