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Three-dimensional cell printing device

A technology for printing devices and three-dimensional cells, which is applied in medical science, prosthesis, etc., can solve the problems of easy contamination of cells, unreliable connection, and high cost, so as to improve the survival rate of cells, reduce the size of the device, and realize desktop Effect

Inactive Publication Date: 2014-04-30
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this forming method is that: the introduction of a crosslinking agent, the crosslinking agent exists on the surface of the cured material unit, resulting in unreliable connection between the manufactured three-dimensional structure layer and the layer, and it is difficult to form a multi-layered three-dimensional cell with a high height. structure
However, the design of related systems is usually to spray the cell material into a low-temperature forming environment, that is, a closed low-temperature refrigerator. This type of refrigerator generally uses a vapor compression refrigeration unit, resulting in a large volume of the forming chamber, a large volume of the entire device, and a high cost. higher
And the cells printed in this device are easily contaminated, and the cell survival rate is low

Method used

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Experimental program
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Effect test

specific Embodiment approach 1

[0016] Such as figure 1 As shown, the three-dimensional cell printing device of this specific embodiment includes a printing nozzle 100 , a first air cooling unit 300 , a second air cooling unit 500 , a forming platform 700 and a refrigeration unit 900 .

[0017] The printing nozzle 100 sucks the printing material from the inkjet cartridge, that is, the temperature-sensitive cell solution in a colloidal state. During printing, the temperature-sensitive cell solution in the sol state is sprayed from the nozzle and printed onto the forming platform 700 .

[0018] The first air-cooling unit 300 and the second air-cooling unit 500 are respectively arranged on both sides of the print nozzle 100, and the first air-cool unit 300 and the second air-cool unit 500 are used to direct cold air to the corresponding print nozzle on the forming platform 700 100 nozzles spray the top of the area. In this specific embodiment, the first air-cooling unit 300 and the second air-cooling unit 500...

specific Embodiment approach 2

[0024] The difference between this specific embodiment and the first embodiment is that the air cooling units 300 and 500 are located directly opposite the nozzles, and guide the cold air horizontally to the top of the area on the forming platform 700 corresponding to the ejection area of ​​the nozzles, while the first embodiment Two air-cooling units in one are positioned obliquely opposite to the nozzles, guiding the cold air at a certain angle to the top of the area on the forming platform 700 corresponding to the spraying area of ​​the nozzles.

[0025] Such as image 3 As shown, it is a schematic structural diagram of the three-dimensional cell printing device in this specific embodiment. The three-dimensional cell printing device includes a printing nozzle 100 , a first air cooling unit 300 , a second air cooling unit 500 , a forming platform 700 and a refrigeration unit 900 . The specific composition, function, and working process of the above-mentioned components are t...

specific Embodiment approach 3

[0027] The difference between this specific embodiment and Embodiments 1 and 2 is that in this specific embodiment, there are two cold source units and a refrigeration unit. The source unit directly provides cooling air to the nozzle area, that is, directly provides cooling for the temperature-sensitive cell solution before spraying. With the refrigeration unit at the bottom, the temperature-sensitive cell solution is refrigerated before spraying out, and it is still in a refrigerated environment after spraying out, thus changing from a sol state to a gel state. In the first two embodiments, there are two air-cooling units and a refrigeration unit. The air-cooling unit and the refrigeration unit form a local cooling environment for the sprayed cell solution, thereby changing from a sol state to a gel state, and completing shaping and solidification.

[0028] Such as Figure 4 As shown, it is a schematic structural diagram of the three-dimensional cell printing device in this ...

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Abstract

The invention discloses a three-dimensional cell printing device which comprises a printing sprayer and a forming platform. The printing sprayer is used for spraying and printing sol cell solution on the forming platform. The three-dimensional cell printing device further comprises two air cooling units and a refrigerating unit or two cold source units and a refrigerating unit. The cell solution is temperature-sensitive. The air cooling units and the refrigerating unit form a local refrigerating forming area covering the printed cell solution or the cold source units and the refrigerating unit allow the cell solution to be in the refrigerated environment before being sprayed and printed and after being sprayed and printed, the temperature-sensitive cell solution is changed into the gel sate, physical crosslinking occurs, and forming printing is achieved. The three-dimensional cell printing device has the advantages that a sealed low-temperature forming chamber and a steam compressing and refrigerating unit can be omitted, the size of the device is reduced greatly, cost is lowered greatly, the multilayer cell three-dimensional structure of cells can be well printed by the device, and cell survival rate can be increased favorably.

Description

【Technical field】 [0001] The invention relates to a three-dimensional cell printing technology in tissue engineering, in particular to a cell printing device adopting refrigeration cross-linking. 【Background technique】 [0002] Bio-3D printing technology is to use the principles and methods of 3D printing to print biological materials (including natural biological materials and synthetic biological materials) or cell solutions into designed 3D structures, and the technology of using cell solution as printing materials is also called cell 3D printing. technology. The current technologies for cell printing include: cell printing based on inkjet printing, cell printing based on laser technology, and cell printing based on three-dimensional printing (rapid prototyping). The first two technologies are difficult to print 3D complex structures, which limits their application in the construction of large-scale 3D bionic structures. The third technique, cell printing based on 3D pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02
Inventor 刘长勇弥胜利刘多静孙伟苏鑫徐圆圆张婷
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV