Method for preparing high strength microorganism mortar by microorganism generating urease and calcium acetate

A technology of calcium acetate and microorganisms, which is applied in the field of high-strength microbial mortar preparation, can solve the problems of steel bar depassivation, electrochemistry, impact on the durability of concrete structures, corrosion, etc., and achieve the effect of short time, low cost and uniform pore size distribution

Active Publication Date: 2014-04-30
TSINGHUA UNIV
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Problems solved by technology

[0005] However, at present, the preparation of high-strength microbial mortar mainly uses calcium chloride as the calcium source, but after chloride ions enter the concrete cracks, it will cause steel bar depassivation and electroc...

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  • Method for preparing high strength microorganism mortar by microorganism generating urease and calcium acetate
  • Method for preparing high strength microorganism mortar by microorganism generating urease and calcium acetate
  • Method for preparing high strength microorganism mortar by microorganism generating urease and calcium acetate

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Embodiment Construction

[0030] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0031] In this embodiment, the method for preparing high-strength microbial mortar using urease-producing microorganisms and calcium acetate, using calcium acetate as a calcium source, specifically includes the following steps:

[0032] Step 1: prepare fermentation medium: yeast extract 20g / L, sulfuric acid presses 10g / L, pH is 9, 100mL fermentation medium is packed in the 500mL culture bottle and sterilized, will produce urease microorganism Pasteurella sarcina Sporosarcina A single colony of pasteurii was inoculated in the fermentation medium, fermented and cultivated at 30°C, with a rotation speed of 150rpm, and cultured for 18 hours to collect the bacterial liquid;

[0033] Step 2: Carry out closed water treatment on the grouting mold, pour sand particles with a particle size of 200-380 microns, close the grouting mold, and reserv...

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Abstract

The invention relates to a method for preparing high strength microorganism mortar by microorganism generating urease and calcium acetate. The method comprises steps of 1. inoculating single bacterial colony of the microorganism generating urease into a fermentation medium for fermentation cultivation so as to obtain a bacterial liquid; 2. processing a grouting die in a closed water mode, pouring sand into the die, sealing the grouting die except the slurry inlet and the slurry outlet; 3. pouring the bacterial liquid into one cubic meter of sand sample from the slurry inlet at the speed of 20-40L/m till a yellow bacterial liquid flows out from the slurry outlet; 4. pouring 0.05-0.1mol/L calcium acetate stationary liquid into one cubic meter of sand sample at the speed of 20-40L/m; 5. pouring a reaction solution into one cubic meter of sand sample for 48 hours at the speed of 2-5L/m; 6. pouring a 0.05-0.1mol/L calcium acetate stationary liquid into one cubic meter of sand sample at the speed of 20-40L/m; and 7. repeating from step 3 to step 6 for many times. The microorganism mortar prepared by the method can reach the required strength in 3-8 days and prevents the harm of chloridion to a concrete structure.

Description

technical field [0001] The invention belongs to the technical field of high-strength microbial mortar preparation, and in particular relates to a method for preparing high-strength microbial mortar by using urease-producing microorganisms and calcium acetate. Background technique [0002] An important application of high-strength microbial mortar prepared by urease-producing microorganisms is the reinforcement of cultural relics and ancient buildings. This technology has also become a new way to repair concrete cracks. [0003] Concrete, as the most important building material in the contemporary era, is widely used in industrial and civil construction, water conservancy, transportation, and seaport projects. However, due to its low tensile strength, micro-cracks are prone to occur on the surface and inside of concrete, and external water and erosive properties The medium will gradually infiltrate from the surface defects of the material, corrode the steel bars, and further ...

Claims

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Application Information

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IPC IPC(8): C04B24/00
Inventor 郭红仙程晓辉化彬张越
Owner TSINGHUA UNIV
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