Method for synthesizing carbon quantum dot by using biologic active enzyme auxiliary microwave method, carbon quantum dot prepared by using method, and application thereof

A technology of biologically active enzymes and carbon quantum dots, which is applied in the fields of nanomaterial science and biomedical engineering, can solve the problems of not having the function of cancer diagnosis and treatment, not being able to enter the nucleus, and low yield, so as to achieve low synthesis cost, high yield, The effect of simple equipment

Inactive Publication Date: 2014-05-07
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Benefits of technology

This patented technology provides methods for producing highly pure fluorochemicai (FCOs), called nanocarbon quantum dods, on demand without harmful chemical substances like heavy metals such as lead. These FCoS particles show promise as diagnostic agents due to its ability to detect diseases through light scattering techniques. They also allow for effective treatments against tumours based on specific molecules involved in pathways regulating gene expression levels. Overall these technical features improve efficiency and effectiveness in various applications related to bioimaging and medical research fields.

Problems solved by technology

This patented technical problem addressed by these inventors relates to finding highly luminescent or conductive polymorphous silica particles useful as probes for medical applications like detecting gene expression levels, identifying specific types of cells, studying metabolism, developing drugs, delivering agents through controlled release systems, exploring potential uses in biomedical science, including cellular therapy and immune response regulation techniques.

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  • Method for synthesizing carbon quantum dot by using biologic active enzyme auxiliary microwave method, carbon quantum dot prepared by using method, and application thereof
  • Method for synthesizing carbon quantum dot by using biologic active enzyme auxiliary microwave method, carbon quantum dot prepared by using method, and application thereof
  • Method for synthesizing carbon quantum dot by using biologic active enzyme auxiliary microwave method, carbon quantum dot prepared by using method, and application thereof

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Embodiment 1

[0040] Using citric acid as carbon precursor and RNase A as biosynthetic template, super-fluorescent carbon quantum dots were prepared.

[0041] Step 1: At room temperature, weigh 1 g of citric acid and 20 mg of RNase A, and measure 5 mL of H 2 0, all moved into a 25mL glass reaction bottle, and the reaction bottle was placed in an ultrasonic wave with a working frequency of 40kHz and a power of 200W for 1min to obtain a uniform aqueous solution;

[0042] Step 2: Put the aqueous solution obtained in Step 1 in a household microwave oven, and microwave it for 4 minutes at a power of 800W, and obtain a brown-yellow liquid after reaction;

[0043]Step 3: Use a dialysis bag with a molecular weight cut-off of 5000 to dialyze the brownish-yellow liquid obtained in step 2. The dialysis time is 24 hours. During the dialysis process, the ultrapure water is continuously replaced to remove unreacted citric acid and RNase A, namely Super fluorescent carbon quantum dots modified by RNase A...

Embodiment 2

[0049] Using glucose as precursor and RNase A as biosynthetic template, high-intensity fluorescent carbon quantum dots were prepared.

[0050] Step 1: At room temperature, weigh 1g glucose and 10mg RNase A, measure 5mL H 2 O, all were moved into a 25mL glass reaction bottle, and the reaction bottle was placed in an ultrasonic wave with a working frequency of 40kHz and a power of 200W for 1min to obtain a uniform aqueous solution.

[0051] Step 2: Put the aqueous solution obtained in Step 1 in a household microwave oven, and heat it in a microwave for 4 minutes at a power of 800W. After the reaction, a brown-yellow liquid is obtained.

[0052] Step 3: Use a dialysis bag with a molecular weight cut-off of 5000 to dialyze the brown-yellow liquid obtained in Step 2. The dialysis time is 36 hours. During the dialysis process, the ultrapure water is continuously replaced to remove unreacted glucose and RNase A. RNase A modified carbon quantum dots were obtained, and the yield of RN...

Embodiment 3

[0055] Laser confocal imaging of gastric cancer MGC-803 cells using RNase AC-dots as fluorescent labels.

[0056] Step 1: Prepare multifunctional fluorescent RNase AC-dots probe labeled with BRCAA1 antibody. Since the surface of RNase AC-dots is rich in carboxyl and amino groups at the same time, the -COOH on the surface of RNase AC-dots particles is activated with cross-linking agents carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The -NH2 on the surface of the BRCAA1 antibody and the -COOH on the surface of the quantum dots were condensed for 2 hours to realize the coupling of the BRCAA1 antibody to the quantum dots. After three washes with PBS buffer, the obtained BRCAA1 antibody-labeled quantum dots were stored at 4°C for future use.

[0057] Step 2: Targeted enrichment of tumor cells. Gastric cancer MGC-803 cells were cultured by conventional in vitro culture methods, and the specific method was as follows: Gastric cancer MGC-803 cells in logarithmic growth phase we...

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Abstract

The invention discloses a method for synthesizing a carbon quantum dot by using a biologic active enzyme auxiliary microwave method. The method specifically comprises the following steps: (1) ultrasonically dispersing a carbon precursor and biologic active enzyme into deionized water, so as to prepare a transparent solution; (2) putting the solution obtained in the step (1) into a domestic micro-oven for heating through microwave, and reacting to obtain claybank liquid which is the biologic active enzyme modified carbon quantum dot; (3) dialyzing the carbon quantum dot solution obtained from the reaction in the step (2) by using a dialysis bag, so as to remove unreacted carbon precursor and biologic active enzyme. The method is high in yield; as the prepared carbon quantum dot is modified by the biologic active enzyme, the carbon quantum dot can enter cell nucleus and has the diagnosis and treatment function on cancer; the biologic active enzyme is used as an active agent and a passivating agent in the reaction, and citric acid, citrate, glucose, fructose or amylase is taken as a carbon precursor, so that the fluorescence carbon quantum dot has good biologic security in the preparation and application process; and the cost is low since the microwave synthesis method is used.

Description

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Claims

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Application Information

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Owner SHANGHAI JIAO TONG UNIV
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