Method for detecting differentially-expressed protein

A differential expression and protein technology, applied in the field of quantitative proteomics, can solve the problems of accuracy evaluation of quantitative results without protein, inaccurate qualitative and quantitative results, labor-consuming and material resources, etc.

Active Publication Date: 2014-05-07
INST OF COMPUTING TECH CHINESE ACAD OF SCI
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Problems solved by technology

[0006] The main problem of the existing analysis methods for protein mass spectrometry data is that the ability to deal with signal overlap and signal interference is weak, and a considerable proportion of the qualitative and quantitative results obtained are inaccurate, and there are almost no algorithms. Evaluate the accuracy of protein quantification results...

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  • Method for detecting differentially-expressed protein
  • Method for detecting differentially-expressed protein
  • Method for detecting differentially-expressed protein

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[0072] The present invention detects which are differentially expressed by analyzing the data of proteins collected by a mass spectrometer in different samples, specifically, the relative quantification of markers and non-markers based on primary spectrum information in quantitative proteomics Data analysis, according to the ratio of the mass spectrometry signal intensity corresponding to a protein in two or more biological samples to determine whether it is a differentially expressed protein.

[0073] Such as figure 1 Shown is the complete implementation process of the present invention, also is the experimental process of marking quantification, mainly comprises:

[0074] (1) Take two different biological samples for differential protein detection. Sample 1 is a normal tissue of a certain organism, which has been labeled with a stable isotope; sample 2 is a biological diseased tissue.

[0075] Use Stable Isotope Labeling by Amino acids in Cell culture (SILAC) or 15 N in vi...

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Abstract

The invention relates to a method for detecting differentially-expressed protein. For quantitative proteomics, on the basis of marked and unmarked relative quantitative data analysis of primary spectrogram information, whether certain protein differentially expresses protein is judged according to corresponding spectrogram signal intensity ratio of the certain protein in two or more samples, and the method comprises the following steps: matching a peptide spectrum, evaluating credibility, extracting peptide fragment signals, calculating a peptide fragment ratio, calculating a protein ratio, and performing statistical analysis. For about 100 GB data collected through mass spectrum experiment, automated analysis is performed rapidly, for signals of different protein in a mass spectrometer, the protein signals can be extracted accurately as soon as possible, protein differential expression is determined from statistical significance, and accuracy of results is evaluated.

Description

technical field [0001] The invention relates to the field of quantitative proteomics, in particular to a method for detecting differentially expressed proteins. Background technique [0002] Quantitative proteomics mainly uses mass spectrometry to study the expression of proteins in complex biological samples, and one of the important research goals is to detect differentially expressed proteins in different biological samples. [0003] For some important proteins, such as the core fucose protein associated with cancer, the expression level in cancer patients is much higher than that in normal people. The differentially expressed core fucose protein can be used as an early diagnostic marker for liver cancer. Differentially expressed proteins play a dominant role in other life processes such as biological signaling, cellular senescence, and post-translational modification. According to reports, in 2011, the member companies of the Pharmaceutical Research and Manufacturers o...

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Application Information

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IPC IPC(8): G01N27/62
Inventor 刘超宋春青迟浩孙瑞祥董梦秋贺思敏
Owner INST OF COMPUTING TECH CHINESE ACAD OF SCI
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