Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Late blight resistance-related proteins and their related biomaterials and applications

A biomaterial and protein technology, applied in the field of late blight resistance-related proteins and related biomaterials, can solve the problems of insensitivity to fungicides, high cost, human health and environmental safety impacts, etc.

Inactive Publication Date: 2015-09-30
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is expensive and has certain negative impacts on human health and environmental safety
In addition, the rapid reproduction of pathogenic bacteria has resulted in some new physiological races being insensitive to commonly used fungicides
This disease is a big problem for organic growers who do not use any chemical pesticides in the production process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Late blight resistance-related proteins and their related biomaterials and applications
  • Late blight resistance-related proteins and their related biomaterials and applications
  • Late blight resistance-related proteins and their related biomaterials and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1, the cloning of SP-LBR3 gene and its functional verification

[0064] 1. Cloning of SP-LBR3 gene

[0065] Genomic DNA of tomato (Solanum lycopersicum) CLN2037B was used as a template, and primers SP-LBR3EF3 (5'-taacctgcaggTTCAAACCATCTTCATAGAGGC-3') and SP-LBR3ER3 (5'-attggcgcgccTGGGGCTTAGAAAAAGGTTG-3') were obtained by using Phusion high-fidelity DNA polymerase (Thermo Fisher, Waltham, MA, USA) PCR amplified the fragment of 4kb upstream of SP-LBR3 gene, SP-LBR3 gene and downstream 2kb of SP-LBR3 gene, and this fragment was called SP-LBR3 gene expression cassette PCR product. The PCR product of the SP-LBR3 gene expression cassette was cloned into pCR-Blunt II-TOPO (Invitrogen, Carlsbad, CA, USA) to obtain a recombinant vector pCR-SP-LBR3 containing the PCR product of the SP-LBR3 gene expression cassette. Sequencing results show that the DNA sequence of the PCR product of the SP-LBR3 gene expression cassette is as SEQ ID No.1, which consists of 8007 nucleoti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a late blight resistance related protein, and a related biomaterial and application thereof. The late blight resistance related protein is the protein as shown in a) or b): a) the protein comprising amino acid sequences as shown in SEQ ID No.2; b) the protein obtained by substituting and / or deleting and / or adding one or more amino acid residues from the amino acid sequences as shown in SEQ ID No.2 and related to the late blight resistance. The late blight resistance related protein gene can be transferred into a receptor of a tomato to obtain a transgenic tomato with the improved late blight resistance, so that the late blight resistance related protein gene is the gene related to the late blight resistance, and can be used to encode the protein related to the late blight resistance.

Description

technical field [0001] The invention relates to a late blight resistance-related protein and related biological materials and applications. Background technique [0002] Late blight, caused by Phytophthora infestans, is one of the most devastating tomato field diseases. Under conditions favorable to the growth of pathogenic bacteria, late blight can spread at an alarming rate, killing the host within 7 to 10 days. Fungicides are currently the most commonly used method for controlling late blight. However, it is expensive and has certain negative effects on human health and environmental safety. In addition, the rapid reproduction of pathogenic bacteria has caused some new physiological races to be insensitive to commonly used fungicides. This disease is a big problem for organic growers who do not use any chemical pesticides in the production process. Therefore, transferring wild tomato disease-resistant genes into cultivated species is an effective method for controllin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N1/15C12N1/19C12N1/21A01H5/00
CPCC07K14/415C12N15/8282
Inventor 郑峥刘磊李君明张春芝杜永臣
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com