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Rapid diagnostic kit for early and mid-stage lung cancer and its detection method

A rapid diagnosis and kit technology, applied in disease diagnosis, measurement devices, instruments, etc., can solve problems such as difficulty in meeting the requirements of early rapid diagnosis, affecting the early detection and treatment of lung cancer, and achieve lower detection costs, high accuracy, The effect of improving detection efficiency

Active Publication Date: 2018-01-30
GUANGZHOU HENGTAI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the single detection of selected tumor markers often has great limitations, it is difficult to meet the requirements of early and rapid diagnosis
[0005] At present, there is no rapid and efficient diagnostic kit for lung cancer on the market, which seriously affects the early detection and treatment of lung cancer

Method used

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  • Rapid diagnostic kit for early and mid-stage lung cancer and its detection method
  • Rapid diagnostic kit for early and mid-stage lung cancer and its detection method
  • Rapid diagnostic kit for early and mid-stage lung cancer and its detection method

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Experimental program
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Embodiment Construction

[0056] The kit for the rapid diagnosis of lung cancer in the early and middle stages of the present invention will be described in detail below in conjunction with the accompanying drawings and specific examples.

[0057] A rapid diagnostic kit for early and mid-stage lung cancer, including capture antibody, capture antibody blocking buffer, standard, HRP-labeled detection antibody, washing solution and chromogenic solution, etc. The capture antibody includes monoclonal antibodies prepared respectively from galectin and cytokeratin fragment antigen 21-1 (CYFRA21-1); ) can effectively improve the detection accuracy and sensitivity, and effectively overcome the shortcomings caused by the detection of CYFRA21-1 alone.

[0058] The capture antibody also includes a monoclonal antibody made from human aspartyl β-hydroxylase (HAAH), through the detection of galectin, cytokeratin fragment antigen 21-1 (CYFRA21-1) and human asparagus Aminoacyl β-hydroxylase (HAAH), making its sensitiv...

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Abstract

The invention discloses a kit used for fast diagnosis of early stage and middle stage lung cancer. Capturing antibodies comprise monoclonal antibodies manufactured by galactose agglutinin, cell cutin segment antigen 21-1, and human aspartyl-beta-hydroxylase respectively, the kit further comprises detection antibodies; the detection antibodies comprises HRP marked polyclonal antibodies which are manufactured by galactose agglutinin, cell cutin segment antigen 21-1, and human aspartyl-beta-hydroxylase respectively. The kit provided by the invention has the following benefits: high sensitivity and good accuracy both higher than 90%, and capability of effectively lowering detection cost, and reducing operating steps and detection errors.

Description

Technical field: [0001] The invention relates to the field of kits for cancer diagnosis and detection, in particular to a kit for early and mid-stage rapid diagnosis of lung cancer and a detection method thereof. Background technique: [0002] Lung cancer is the cancer with the highest mortality rate worldwide. This type of cancer is difficult to treat, and metastatic lung cancer is particularly difficult to treat. About 75% of lung cancer patients are already metastatic or advanced when diagnosed, and the 5-year survival rate is only 6%. Although chemotherapy, radiotherapy and surgery have developed rapidly in the past decades, the 5-year survival rate of lung cancer is still low. Mainly because there is no safe way to screen for it, the chances of finding a patient in time for lung cancer are slim to none in the early stages of the disease—the best time for treatment. [0003] An effective screening strategy is urgently needed. When CT scans are unclear, blood tests ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
CPCG01N33/57423G01N2800/12
Inventor 余跃飞
Owner GUANGZHOU HENGTAI BIOTECH