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A kind of production method of biodiesel grease

A biodiesel and production method technology, applied in the chemical industry, can solve the problem of high cost, and achieve the effects of eliminating pretreatment costs, saving land and protecting the environment

Active Publication Date: 2016-04-13
上海中器环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, microbial production of biodiesel has not yet achieved industrial production, mainly due to the high cost

Method used

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  • A kind of production method of biodiesel grease

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Experimental program
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Effect test

Embodiment 1

[0020] The oil-rich chlorella algae species were inoculated into sterile f / 2 medium through aseptic operation, placed in a light incubator, culture temperature 25°C, light intensity 2000lx, culture for 10 days. The oil-rich Cryptococcus flexus strains were inoculated into sterile shake flask culture solution (mg / L): yeast extract 3000, malt extract 3000, peptone 5000 and glucose 20000, placed in a shaker. Cultivation in bed, rotation speed 200 rpm, culture temperature 35°C, culture for 72 hours. 50g of oil-rich chlorella grown in a light incubator and 50g of oil-rich Cryptococcus curvy fermented in a shaker were centrifuged (8000 rpm, 10 minutes) to collect the biomass and rinsed with distilled water for 5 Times. Pretreatment with cytolytic enzyme L2524-25KU: oleaginous microbial biomass (dry matter) and cytolytic enzyme L2524-25KU are mixed at a mass ratio of 100:1, 45°C, initial pH 6.0, stirring speed 150 rpm ,4 hours. Use n-hexane and ethanol to extract oil and fat. The v...

Embodiment 2

[0024] The oil-rich prokaryotic chlorella algae was inoculated into sterile f / 2 medium through aseptic operation, and placed in a light incubator for culture at a culture temperature of 28° C. and a light intensity of 5000 lx for 10 days. Inoculate the oil-rich Rhodotorula glutinosa strain into the sterile shake flask culture medium through aseptic operation: 1000mL potato extract (peel 200g potatoes, cut into small pieces, add water and boil for 1 hour, filter with 5 layers of gauze, and dilute to 1000mL ) And glucose 20000mg / L, cultured in a shaker, rotating speed 150 rpm, culture temperature 37 ℃, culture for 72 hours. Separately, 50g of oil-rich Prokaryotic Chlorella grown in a light incubator and 50g of oil-rich Rhodotorula glutinosa fermented in a shaker were centrifuged (8000 rpm, 10 minutes) to collect the biomass and rinse with distilled water 5 times. Pretreatment with cytolytic enzyme L2524-10KU: oleaginous microbial biomass (dry matter) and cytolytic enzyme L2524-1...

Embodiment 3

[0028] The oil-rich Pseudochlorococcum algae species were inoculated into sterile f / 2 medium through aseptic operation, placed in a light incubator, culture temperature 25°C, light intensity 2000lx, culture for 10 days. The oil-rich Mortierella alpina was inoculated into a sterile shake flask culture medium (mg / L): yeast extract 10000, peptone 10000 and glucose 30,000, cultured in a shaker at 180 revolutions / Min, culture temperature 37°C, culture for 72 hours. Separately, 50g of oil-rich Pseudochlorococcum grown in a light incubator and 50g of oil-rich Mortierella alpina fermented in a shaker were centrifuged (8000 rpm, 10 minutes) to collect the biomass and rinse with distilled water 5 times. Pretreatment with cytolytic enzyme L4025-100KU: oleaginous microbial biomass (dry matter) and cytolytic enzyme L4025-100KU are mixed at a mass ratio of 50:1, 45°C, initial pH 6.0, and stirring speed 150 rpm ,4 hours. Use n-hexane and ethanol to extract oil and fat. The volume ratio of...

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Abstract

The invention discloses a production method of biodiesel oil. The production method comprises the following steps: (1), carrying out enzymolysis onto microbial oil-extraction leftover materials by cytase to obtain enzymatic hydrolysate of microbial oil-extraction leftover materials; (2), mixing water with the enzymatic hydrolysate in a mass ratio of (5-3):1, and adding magnesium sulfate, ferric chloride and sodium dihydrogen phosphate to prepare a heterotrophic microbial fermentation culture medium; (3), inoculating oil-production heterotrophic microbes in a fermentation culture medium in the step (2) to ferment; and inoculating oil-production autotrophic microbes in a sterilized f / 2 culture medium by virtue of sterile operation to carry out third-stage cultivation; and (4), collecting oil-production microbial biomass in the step (3), pre-treating, and extracting oil from normal hexane, alcohol and pre-treated microbial biomass in a volume ratio of 1:1:(1-3), carrying out rotary evaporation onto an organic phase at speed of 20 revolutions / minute-80 revolutions / minute under a temperature of 30 DEG C-50 DEG C, removing a solvent, removing impurities, and drying to obtain the microbial oil. The production method disclosed by the invention improves oil production efficiency, lowers production cost, is environment-friendly and land-saving, and protects the environment.

Description

Technical field [0001] The invention relates to the field of chemical technology. Background technique [0002] Renewable bioenergy produced by microorganisms can be used as one of the beneficial supplements and alternatives to fossil energy, but it has not yet fully realized its potential. Microbial grease, also known as single cell grease, is a good raw material for preparing biodiesel. As an energy raw material, microbial oil has the following advantages: to ensure energy security and reduce dependence on reserves of petrochemical energy. Compared with terrestrial plants, the use of microorganisms to produce bioenergy has the following advantages: First, microorganisms grow and multiply quickly, and require less area for cultivation. At the same time, microbial cultivation can use non-arable land such as tidal flats and waste land. Therefore, the use of microorganisms to produce biological energy Energy will not cause world food supply problems. Furthermore, the growth proc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
CPCY02E50/10
Inventor 郑洪立马小琛阮榕生刘玉环高振彭红黄和刘君英张锦胜
Owner 上海中器环保科技有限公司
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