A kind of tea saponin biological enzyme decolorization process
A tea saponin and bio-enzyme technology, which is applied in the field of tea saponin bio-enzyme decolorization process, can solve the problems of tea saponin loss recovery, operator injury, environmental pollution, etc., and achieve low loss rate, mild reaction conditions, and fast decolorization time short effect
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Embodiment 1
[0025] （ 1 ) Co-immobilization of laccase and glucose oxidase : Mix equal volumes of 0.05 wt %% laccase aqueous solution and 0.05 wt % glucose oxidase aqueous solution, mix and stir with 5 wt % sodium alginate solution at a volume ratio of 1:7, then add sodium alginate, etc. The volume of 5wt% gelatin solution was uniformly mixed, and the pH was adjusted to 4. After stirring at a slow speed for 80 minutes, the temperature of the mixture was lowered to 10°C, and then the mixture was injected into 3% calcium chloride solution at a height of 7cm through the needle of the syringe. Microspheres were formed in the medium, and then kept at a temperature of 4±1°C. After hardening for 8 hours, the microspheres were taken out and placed in a 5 wt % glutaraldehyde solution at 30°C for further hardening for 1 hour, and then washed with normal saline to remove residual glutaraldehyde. , to obtain co-immobilized laccase and glucose oxidase;
[0026] （ 2 ) Preparation of crude tea sap...
Embodiment 2
[0030] （ 1 ) Co-immobilization of laccase and glucose oxidase: Mix equal volumes of 0.08 wt %% laccase aqueous solution and 0.08 wt % glucose oxidase aqueous solution, mix and stir with 5 wt % sodium alginate solution at a volume ratio of 1:10, then add sodium alginate, etc. The volume of 5wt% gelatin solution was uniformly mixed, and the pH was adjusted to 4. After stirring slowly for 90 minutes, the mixture was cooled to 8°C, and then the mixture was injected into 5% calcium chloride solution at a height of 10cm through the needle of the syringe. The microspheres were formed in the medium, and then the temperature was kept at 4 °C. After hardening for 10 h, the microspheres were taken out and placed in a 5 wt % glutaraldehyde solution at 30 °C for further hardening for 2 h, and then washed with normal saline to remove the residual glutaraldehyde to obtain Co-immobilized laccase and glucose oxidase;
[0031] （ 2 ) Preparation of crude tea saponin solution : dissolving ...
Embodiment 3
[0035] （ 1 ) Co-immobilization of laccase and glucose oxidase : Mix 0.1 wt %% laccase aqueous solution and 0.1 wt % glucose oxidase aqueous solution in equal volumes, mix and stir with 6 wt % sodium alginate solution at a volume ratio of 1:9, then add sodium alginate, etc. The volume of 6wt% gelatin solution was evenly mixed, and the pH was adjusted to 5. After stirring slowly for 100 minutes, the mixture was cooled to 5°C, and then the mixture was injected into 5% calcium chloride solution at a height of 5cm through the needle of the syringe. Microspheres were formed in the medium, and then kept at 4°C. After hardening for 8 hours, the microspheres were taken out and placed in a 30±2°C, 6 wt % glutaraldehyde solution for further hardening for 1 hour, and then washed with normal saline to remove residual glutaraldehyde. , to obtain co-immobilized laccase and glucose oxidase;
[0036] （ 2 ) Preparation of crude tea saponin solution : dissolving crude tea saponin in aceti...
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