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A kind of tea saponin biological enzyme decolorization process

A tea saponin and bio-enzyme technology, which is applied in the field of tea saponin bio-enzyme decolorization process, can solve the problems of tea saponin loss recovery, operator injury, environmental pollution, etc., and achieve low loss rate, mild reaction conditions, and fast decolorization time short effect

Active Publication Date: 2016-08-10
HANGZHOU YIMIN PETOROCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the existing problems in the prior art, which may cause harm to the operator and pollute the environment under strong alkali conditions, or cause great losses and difficult recovery of tea saponin due to adsorption, and provide An environmentally friendly tea saponin recovery and low loss rate tea saponin enzyme decolorization process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] （ 1 ) Co-immobilization of laccase and glucose oxidase : Mix equal volumes of 0.05 wt %% laccase aqueous solution and 0.05 wt % glucose oxidase aqueous solution, mix and stir with 5 wt % sodium alginate solution at a volume ratio of 1:7, then add sodium alginate, etc. The volume of 5wt% gelatin solution was uniformly mixed, and the pH was adjusted to 4. After stirring at a slow speed for 80 minutes, the temperature of the mixture was lowered to 10°C, and then the mixture was injected into 3% calcium chloride solution at a height of 7cm through the needle of the syringe. Microspheres were formed in the medium, and then kept at a temperature of 4±1°C. After hardening for 8 hours, the microspheres were taken out and placed in a 5 wt % glutaraldehyde solution at 30°C for further hardening for 1 hour, and then washed with normal saline to remove residual glutaraldehyde. , to obtain co-immobilized laccase and glucose oxidase;

[0026] （ 2 ) Preparation of crude tea sap...

Embodiment 2

[0030] （ 1 ) Co-immobilization of laccase and glucose oxidase: Mix equal volumes of 0.08 wt %% laccase aqueous solution and 0.08 wt % glucose oxidase aqueous solution, mix and stir with 5 wt % sodium alginate solution at a volume ratio of 1:10, then add sodium alginate, etc. The volume of 5wt% gelatin solution was uniformly mixed, and the pH was adjusted to 4. After stirring slowly for 90 minutes, the mixture was cooled to 8°C, and then the mixture was injected into 5% calcium chloride solution at a height of 10cm through the needle of the syringe. The microspheres were formed in the medium, and then the temperature was kept at 4 °C. After hardening for 10 h, the microspheres were taken out and placed in a 5 wt % glutaraldehyde solution at 30 °C for further hardening for 2 h, and then washed with normal saline to remove the residual glutaraldehyde to obtain Co-immobilized laccase and glucose oxidase;

[0031] （ 2 ) Preparation of crude tea saponin solution : dissolving ...

Embodiment 3

[0035] （ 1 ) Co-immobilization of laccase and glucose oxidase : Mix 0.1 wt %% laccase aqueous solution and 0.1 wt % glucose oxidase aqueous solution in equal volumes, mix and stir with 6 wt % sodium alginate solution at a volume ratio of 1:9, then add sodium alginate, etc. The volume of 6wt% gelatin solution was evenly mixed, and the pH was adjusted to 5. After stirring slowly for 100 minutes, the mixture was cooled to 5°C, and then the mixture was injected into 5% calcium chloride solution at a height of 5cm through the needle of the syringe. Microspheres were formed in the medium, and then kept at 4°C. After hardening for 8 hours, the microspheres were taken out and placed in a 30±2°C, 6 wt % glutaraldehyde solution for further hardening for 1 hour, and then washed with normal saline to remove residual glutaraldehyde. , to obtain co-immobilized laccase and glucose oxidase;

[0036] （ 2 ) Preparation of crude tea saponin solution : dissolving crude tea saponin in aceti...

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Abstract

The invention discloses a tea saponin bio-enzyme decolorization process, and its purpose is to solve the existing problems in the prior art, which may cause harm to the operator and pollute the environment under strong alkali conditions, or cause tea soap decolorization due to adsorption. The problem of great loss of prime and difficult recovery. The invention includes co-immobilization of laccase and glucose oxidase, preparation of crude tea saponin solution, decolorization of crude tea saponin, recovery of tea saponin and the like. The invention has mild reaction conditions, short decolorization time and remarkable decolorization effect, and the immobilized enzyme can be recovered and reused, the loss rate of the tea saponin is low, and the tea saponin powder with low color can be recovered and obtained only by spray drying, and the process is simple.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a tea saponin biological enzyme decolorization process. Background technique [0002] Tea saponin is a pentacyclic triterpenoid glycoside compound extracted from the Theaceae plant. It is a nonionic surfactant with excellent performance and has strong emulsifying, dispersing, moisturizing, foaming, and foaming functions. It can be widely used in the fields of light industry, chemical industry, pesticide, feed, textile, mining and construction, etc., and can be used in the manufacture of emulsifiers, detergents, pesticide auxiliaries, feed additives, textile auxiliaries and coagulant inhibitors, etc. At present, the powder obtained by spray-drying the crude extract of tea saponin is used more, which contains a large amount of Maillard pigments, polyphenols and flavonoid pigment substances, and the aqueous solution of 5% crude extract of tea saponin is the color The deep tan color se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/06
Inventor 王莉
Owner HANGZHOU YIMIN PETOROCHEM
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