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Coxiella burnetii nucleic acid detection kit

A technology of Coxelite and kits, which is applied in the determination/inspection of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of high detection costs, achieve high sensitivity, be conducive to industrialization, and specificity strong effect

Inactive Publication Date: 2014-06-25
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These techniques have higher specificity, but require professional technicians and equipment to implement, and the detection cost is relatively high

Method used

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  • Coxiella burnetii nucleic acid detection kit
  • Coxiella burnetii nucleic acid detection kit
  • Coxiella burnetii nucleic acid detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The following examples are intended to illustrate rather than limit the invention.

[0036] Detection method of the nucleic acid detection kit

[0037] 1. Collection of samples

[0038] a) Serum collection: Take 2ml of venous blood from the subject, collect it in a sterile centrifuge tube, place it at room temperature for no more than 4 hours, centrifuge at 3000rpm for 20 minutes, absorb the serum and transfer it to another sterile centrifuge tube for later use;

[0039] b) Plasma collection: Take 2ml of venous blood from the subject and place it in a sterile centrifuge tube containing anticoagulant (heparin anticoagulant is prohibited), leave it at room temperature for no more than 4 hours, centrifuge at 3000rpm for 20 minutes, separate the plasma, and transfer it to a sterile tube. The centrifuge tube is spare.

[0040] 2. Nucleic acid extraction

[0041] 2.1 Nucleic acid extraction

[0042] a) Pipette 100 μl of sample treatment solution A into a centrifuge tube w...

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Abstract

The invention relates to a Coxiella burnetii nucleic acid detection kit and particularly relates to a kit for detecting Coxiella burnetii nucleic acid by utilizing a composite probe real-time fluorescent PCR (polymerase chain reaction) technology. The kit carries out qualitative detection on the Coxiella burnetii in a sample, and can be applied to auxiliary diagnosis of Coxiella burnetii infection.

Description

technical field [0001] The invention relates to a nucleic acid detection kit for Coxiella beinii, in particular to a kit for detecting Coxiella bezii by real-time fluorescent PCR technology of composite probes. The kit can be applied to the auxiliary diagnosis of infection containing Coxella bainiferi through the qualitative detection of Coxiella baziae in samples. Background technique [0002] Coxiella burnetii (CB) is the pathogen of Q fever, a common zoonotic natural foci disease, and is a Gram-negative bacterium that strictly parasitizes living cells. The pathogen has strong resistance to physical and chemical factors, can survive for a long time in different external environments, and proliferates in the phagolysosome of the host cell, and the suitable pH value is 4.5-5.0. Usually transmitted through aerosols, humans and animals are generally susceptible, and it can cause a worldwide epidemic. However, animals generally have no clinical manifestations except for occas...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/686C12Q2563/107
Inventor 陈苏红王升启罗琳刘志红孙晓彦刘琦琪
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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