Method for assembling nanoparticle carrying laminin and sdf-1α on the surface of ti material

A technology of laminin and SDF-1, which is applied in the direction of surface coating liquid devices, special surfaces, coatings, etc., can solve the problems of limited application, insufficient blood compatibility, etc., and achieves wide application range and excellent preparation process And the fixation method is simple and easy to operate, and the effect of inhibiting thrombus formation

Inactive Publication Date: 2016-01-06
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the polyDM layer still has the disadvantage of insufficient hemocompatibility, which limits its application in the field of cardiovascular materials.
However, there is no report on the assembly of laminin-loaded Hep-PLL nanoparticles and SDF-1α on the surface of titanium materials.

Method used

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  • Method for assembling nanoparticle carrying laminin and sdf-1α on the surface of ti material
  • Method for assembling nanoparticle carrying laminin and sdf-1α on the surface of ti material
  • Method for assembling nanoparticle carrying laminin and sdf-1α on the surface of ti material

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Experimental program
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Effect test

Embodiment 1

[0026] see figure 1 , the first embodiment of the present invention is a method for loading laminin nanoparticles and SDF-1α on the surface of a Ti material, the steps of which are:

[0027] A, polydopamine is deposited on the surface of cardiovascular metal Ti material to deposit polydopamine coating, and dry at 37°C;

[0028] B. Preparation of laminin-loaded nanoparticles Add an equal volume of laminin solution with a concentration of 30 μg / ml to a heparin sodium solution with a concentration of 10 mg / ml, and let stand at 37°C for 1 hour; Under stirring conditions, add an equal volume of heparin sodium and laminin mixture dropwise to a polylysine (PLL, MW150-300KDa) solution with a concentration of 0.3mg / ml;

[0029] C. Nanoparticle immobilization Soak the sample deposited with polydopamine in step A in the nanoparticle suspension obtained in step B, react under shaking conditions at 50°C for 24 hours, and wash with phosphate buffered saline (PBS) and double distilled water...

Embodiment 2

[0032] A method for loading laminin-loaded nanoparticles and SDF-1α on the surface of a Ti material, the steps of which are:

[0033] A, polydopamine is deposited on the surface of the metal Ti material to deposit a polydopamine coating, and dry at 37 ° C;

[0034] B. Preparation of laminin-loaded nanoparticles Add an equal volume of laminin solution with a concentration of 300 μg / ml to a heparin sodium solution with a concentration of 30 mg / ml, and let stand at 37°C for 3 hours; Under stirring conditions, add an equal volume of heparin sodium and laminin mixture dropwise to a polylysine (PLL, MW150-300KDa) solution with a concentration of 1.0mg / ml;

[0035] C. Nanoparticle immobilization Soak the sample deposited with polydopamine in step A in the nanoparticle suspension obtained in step B, react under shaking conditions at 15°C for 6 hours, and wash with phosphate buffered saline (PBS) and double distilled water respectively Rinse and save for later use;

[0036] D. Assemb...

Embodiment 3

[0038] A method for loading laminin-loaded nanoparticles and SDF-1α on the surface of a Ti material, the steps of which are:

[0039] A, polydopamine is deposited on the surface of the metal Ti material to deposit a polydopamine coating, and dry at 37 ° C;

[0040] B. Preparation of laminin-loaded nanoparticles Add an equal volume of laminin solution with a concentration of 200 μg / ml to a heparin sodium solution with a concentration of 20 mg / ml, and let stand at 37°C for 2 hours; Under stirring conditions, add an equal volume of heparin sodium and laminin mixture dropwise to a polylysine (PLL, MW150-300KDa) solution with a concentration of 0.5mg / ml;

[0041] C. Nanoparticle immobilization Soak the sample deposited with polydopamine in step A in the nanoparticle suspension obtained in step B, react under shaking conditions at 37°C for 12 hours, and wash with phosphate buffered saline (PBS) and double distilled water respectively Rinse and save for later use;

[0042] D. Assembl...

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Abstract

The invention discloses a method for assembling nano-particles carrying laminin and SDF-1alpha on surfaces of Ti materials. The method comprises the steps that firstly, Hep-PLL nano-particles carrying Ln are prepared; then DM coatings are prepared on the surfaces of angiocarpy materials, nano-particles including amidogen are fixed to surfaces of samples in a covalence mode through the characteristics that DM and the amidogen can generate the Michael reaction and the Sievert reaction; finally, through the characteristics that heparin in the nano-particles can be specially bound with the SDF-1alpha, the SDF-1alpha is assembled to the surfaces of the nano-particles, and therefore multifunctional bioid modification surfaces with anticoagulation, hyperplasia prevention and endothelium regeneration inducing characteristics are constructed. According to the method, the nano-particle modification layers with the anticoagulation, hyperplasia prevention and endothelium regeneration inducing characteristics are constructed on the surfaces of the angiocarpy materials, the blood compatibility and endothelium and endothelial progenitor cell compatibility of the materials are obviously improved, and the capacity of restraining smooth muscle hyperplasia is enhanced.

Description

technical field [0001] The invention relates to nanoparticle preparation technology and inorganic material surface modification technology, in particular to a method for surface biochemical modification of cardiovascular implant materials. Background technique [0002] Cardiovascular materials need to have excellent anticoagulant ability because they are in direct contact with blood after implantation. In addition, for special cardiovascular implants such as vascular stents, on the basis of good blood compatibility, it should also have the ability to inhibit the excessive proliferation of vascular intima tissue and promote the rapid repair of the endothelial layer. [0003] By biochemically modifying the surface of cardiovascular materials, endowing them with good anticoagulant ability and vascular endothelial regeneration ability is an effective way to improve their biocompatibility. Cardiovascular materials are often inorganic materials, which do not have the characterist...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B05D5/00B05D7/14B05D7/24
Inventor 陈俊英刘涛刘阳王健黄楠曾峥魏来王媛刘诗卉张琨陈佳龙
Owner SOUTHWEST JIAOTONG UNIV
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