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Application of a kind of polysaccharide from sea plate car

A technology of polysaccharides and polysaccharides, which is applied in the application field of polysaccharides of sea pans, and can solve problems such as few

Active Publication Date: 2015-11-11
南通中国科学院海洋研究所海洋科学与技术研究发展中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many studies on the polysaccharides of Haipanche
A small amount of literature has reported that the acidic mucopolysaccharides of sea peony have anticoagulant effects, inhibiting erythrocyte aggregation and thrombus formation, but there are few reports on other polysaccharides and biological activities in sea peony

Method used

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  • Application of a kind of polysaccharide from sea plate car
  • Application of a kind of polysaccharide from sea plate car
  • Application of a kind of polysaccharide from sea plate car

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Preparation of Roche sea car polysaccharide

[0017] (1) Crush 200g of dried Roche sea pan, treat it twice with 3 times the volume of isopropanol, filter, discard the isopropanol, then continue to treat the sea pan with 3 times the volume of ethanol twice, filter , discard the ethanol, and dry the sea tray. The yield was 80%.

[0018] (2) Extract 160g of degreased sea pan with 3 times the volume of 0.1M HCl solution for 2 hours, filter, adjust the pH of the extract to 5-8 with alkali, concentrate, dialyze with a dialysis bag with a molecular weight cut-off of 3500Da for 2 days, and concentrate the dialysis The solution in the bag was freeze-dried to obtain 18.02 g of polysaccharides (expressed as SF-1), with a yield of 9.01%.

[0019] See Table 1 for the chemical composition analysis, monosaccharide ratio analysis, sulfate content, and molecular weight determination results of the polysaccharides of Roche Haipanche. The results of IR spectrum showed that S...

Embodiment 2

[0020] Example 2: Preparation of polysaccharides from sea thorns

[0021] (1) Crush 200g of dried sea thorns, treat twice with 3 times the volume of isopropanol, filter, discard the isopropanol, and then continue to treat the sea plate with 3 times the volume of ethanol twice, Filter, discard the ethanol, and dry the carton. The yield was 84%.

[0022] (2) Extract 168g of degreased sea pan with 3 times the volume of 0.01M HCl solution for 3 hours, filter, adjust the pH of the extract to 5-8 with alkali, concentrate, and dialyze with a dialysis bag with a molecular weight cut-off of 3500Da for 2 days, concentrate and dialysis The solution in the bag was added to edible ethanol until the final concentration of ethanol was 75%, and a white precipitate was obtained. The precipitate was washed with 95% ethanol, and dried under reduced pressure to obtain 20.3 g of polysaccharides (expressed as SF-2). 10.15%.

[0023] See Table 1 for the chemical composition analysis, monosacchari...

experiment example 1

[0026] Experimental Example 1: Protective effect of the SF-1 and SF-2 samples in the above-mentioned Example 1 and Example 2 on dopaminergic nerve cells damaged by neurotoxin 6-OHDA.

[0027] In this experiment, the cell survival rate was measured by MTT method to reflect the toxicity of each sample against neurotoxin 6-OHDA, and to illustrate its role in neuroprotection.

[0028] Test samples: polysaccharide SF-1 prepared in Example 1 and the polysaccharide SF-2 prepared in Example 2.

[0029] Take MES23.5 dopaminergic cells in the logarithmic growth phase, blow and blow to make a single cell suspension, centrifuge, dilute with complete medium to form a cell suspension of 2×105 cells / ml, inoculate 200 μl per well in a 96-well plate, Cultured in a 37°C, 5% CO2 incubator. After adhering to the wall, add Dulbecco's modification of Eagle's medium / F12 (DMEM / F12) culture solution (Gibco company) to test samples (concentration 1 mg / ml, 0.1 mg / ml) and 6-OHDA (100 μmol / L) to co-cultu...

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Abstract

The present invention provides an application of asterias rollestoni polysaccharides in prevention and treatment of neurodegenerative diseases including parkinson disease and senile dementia. According to the present invention, the asterias rollestoni polysaccharides are derived from Asierias rollestoni Bell and Asterias amurensis and are extracted by using a diluted acid, and filtration, desalination and other steps are performed to prepare the asterias rollestoni polysaccharides; and the asterias rollestoni polysaccharides have significant neuroprotection effects and provide significant repair effects for two cell lines damaged by neurotoxin, such that the asterias rollestoni polysaccharides can be used for preparation of drugs and health products for treatment of nervous system diseases such as parkinson disease and senile dementia.

Description

technical field [0001] The present invention relates to the application of polysaccharides of spiky caramel in the preparation of medicines or health care products for treating Parkinson's disease and senile dementia. Background technique [0002] With the average life expectancy of human beings increasing year by year, the world is entering the era of global aging. Age-related neurodegenerative diseases also increase accordingly. In terms of the central nervous system, the main manifestations are weakened excitation and inhibition processes, reduced brain function, memory loss and loss, followed by progressive decline in recognition function and aggravation of emotional disorders Wait. Senile dementia has become the fourth disease after cardiovascular and cerebrovascular diseases and tumors. Currently, the prevalence of senile dementia among people over 65 years old worldwide is about 5%. Due to its complex pathogenesis, it is impossible to make a breakthrough in the pre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/736A61P25/28A23L1/29A23L33/00
Inventor 张全斌张文静王晶金维华
Owner 南通中国科学院海洋研究所海洋科学与技术研究发展中心
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