Self-assembling method for preparing fibroin microspheres

A self-assembly, silk fibroin technology, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human peptides, etc. Structural features, simple preparation process, and the effect of improving utilization

Inactive Publication Date: 2014-08-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of traditional methods (such as emulsification method, spray drying method, laminar jet method) to remove the organic solvent added during the preparation process will cause toxicity problems; in addition, the prepared microspheres have a large particle size, and the preparation conditions require high pressure High temperature destroys protein structure

Method used

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  • Self-assembling method for preparing fibroin microspheres
  • Self-assembling method for preparing fibroin microspheres
  • Self-assembling method for preparing fibroin microspheres

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The preparation method of silk fibroin microspheres in this embodiment includes the following steps in sequence.

[0027] (1) Cut the silkworm cocoon into pieces, weigh 20g and put it into 0.5% Na by mass at a bath ratio of 1:00 2 CO 3 in aqueous solution. Degumming was carried out in an aqueous solution, and after degumming for 30 minutes, it was washed twice with deionized water; the above steps were repeated, and after three times of washing with deionized water, the silk fibroin sample was flattened and dried in an oven at 50°C to obtain a silk fibroin sample. Silk fibroin fiber; the Na 2 CO 3 The temperature of the aqueous solution is 85-105°C.

[0028] (2) Weigh 10g of silkworm silk fiber in step (1) and add it to LiBr solution with a concentration of 9mol / L and place it in a shaker for 6 hours to accelerate the dissolution to obtain a silk fibroin solution; put the silk fibroin solution on the dialysis membrane Dialyzed with deionized water for 4 days to obt...

Embodiment 2

[0035] (1) Cut the silkworm cocoon into pieces, weigh 20g and put it into 0.5% Na by mass at a bath ratio of 1:00 2 CO 3 in aqueous solution. Degumming was carried out in an aqueous solution, and after degumming for 30 minutes, it was washed twice with deionized water; the above steps were repeated, and after three times of washing with deionized water, the silk fibroin sample was flattened and dried in an oven at 50°C to obtain a silk fibroin sample. Silk fibroin fiber; the Na 2 CO 3 The temperature of the aqueous solution is 85-105°C.

[0036] (2) Weigh 10g of silkworm silk fiber in step (1) and add it to the LiBr solution with a concentration of 9mol / L and place it in a shaker for 5 hours to accelerate the dissolution to obtain a silk fibroin solution; put the silk fibroin solution on the dialysis membrane Dialyzed with deionized water for 4 days to obtain a relatively pure silk fibroin solution, which was naturally air-dried and filtered to obtain a pure silk fibroin s...

Embodiment 3

[0043] (1) Cut the silkworm cocoon into pieces, weigh 20g and put it into 0.5% Na by mass at a bath ratio of 1:00 2 CO 3 in aqueous solution. Degumming was carried out in an aqueous solution, and after degumming for 30 minutes, it was washed twice with deionized water; the above steps were repeated, and after three times of washing with deionized water, the silk fibroin sample was flattened and dried in an oven at 50°C to obtain a silk fibroin sample. Silk fibroin fiber; the Na 2 CO 3 The temperature of the aqueous solution is 85-105°C.

[0044] (2) Weigh 10g of silkworm silk fiber in step (1) and add it to the LiBr solution with a concentration of 9mol / L and place it in a shaker for 5 hours to accelerate the dissolution to obtain a silk fibroin solution; put the silk fibroin solution on the dialysis membrane Dialyzed with deionized water for 4 days to obtain a relatively pure silk fibroin solution, which was naturally air-dried and filtered to obtain a pure silk fibroin s...

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Abstract

The invention discloses a self-assembling method for preparing fibroin microspheres. The method comprises the following steps: shearing cocoon shells of silkworms into pieces, putting the sheared cocoon shells into a Na2CO3 water solution to be subjected to degumming treatment, and cleaning with deionized water; putting the cleaned fibroin sample into the Na2CO3 water solution to be subjected to the degumming treatment again, cleaning with deionized water, then flatly spreading the fibroin sample in a baking oven, drying to obtain silkworm fibroin fiber and the like until a milk white liquid is obtained, and then freeze-drying into powder, so that a finished product can be prepared. According to the invention, the fibroin microspheres are prepared without adding any poisonous chemical cross-linking agents, the preparation technology is simple, the reaction condition is moderate, and the fact that the structural characteristics of silk fibroin is kept is facilitated, the utilization ratio of silk fibroin is increased, the production cost is reduced, and the yield of fibroin microspheres is increased. The fibroin microspheres prepared by adopting the self-assembling method can be applied to the fields of medicine carrying micro-system, cellular uptake and the like, and a new thought and a new method are provided for the development of biomedicine.

Description

technical field [0001] The invention belongs to a preparation method of silk fibroin microspheres, in particular to a self-assembly method of silk fibroin microspheres. Background technique [0002] Silk fibroin is the most important component of silk, accounting for about 2 / 3. It is composed of 18 kinds of amino acids such as glycine, alanine, and serine. It is a natural protein because of its good mechanical properties, biocompatibility, Biodegradability, breathability, oxygen permeability, and moisture absorption and moisturizing properties have been widely studied and applied in the fields of biomedicine, cosmetics, and food ([J] Progress in Polymer Science, 2007, 32:991-1007) . Silk fibroin microspheres prepared from silk fibroin have the advantages of large specific surface area, good biocompatibility, biodegradability, good fluidity and diffusibility, etc., so they have been widely studied. The traditional methods for preparing silk fibroin microspheres mainly inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/14
CPCC07K14/43586
Inventor 朱良均潘岳林张海萍杨明英邓连霞
Owner ZHEJIANG UNIV
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