Preparation method for silk fibroin composite gel

Abstract

The invention discloses a preparation method for silk fibroin composite gel. The preparation method comprises the following steps: conducting a dissolution treatment on silk fibroin obtained by degumming in a LiBr solution; preparing a regenerated fibroin solution through dialysis; mixing the regenerated fibroin solution with Gamma- polyglutamic acid; finally, preparing the silk fibroin composite gel under the action of a cross linking agent. The preparation method is simple in technology and short in the gelation time; the efficiency is greatly improved; the required amount of the cross linking agent is small; the safety is strong; the prepared silk fibroin composite gel is degradable and has relatively high mechanical property and stronger water absorbability, so that the silk fibroin composite gel is superior to silk fibroin composite gel prepared from other methods. The silk fibroin composite gel can be used for the fields of medicine and health, tissue engineering, environmental protection and the like; the application of the silk fibroin composite gel is broadened and a novel thought and a novel method of preparing high-strength silk fibroin composite gel are provided.

Description

Technical field [0001] The invention belongs to a processing technique of a regenerated silk fibroin solution, and specifically, is a preparation method of a composite gel with higher mechanical properties and water absorption properties. Background technique [0002] The regenerated silk fibroin solution is an unstable state, even if there is no external effect, there will be a transition from sol to gel. Under normal circumstances, this transformation requires a long time and the resulting gel has poor mechanical properties ([J]Biomacromolecules,2004,5(3):786-792), which greatly limits silk fibroin as an excellent organism The application of materials, so the modification of silk fibroin gel, including physical blending and chemical cross-linking, has become a research hotspot, mainly for improving the mechanical properties and stability of the gel. However, at present, there is no method to prepare silk fibroin gel that can exhibit higher mechanical properties with a simpler ...

AI Technical Summary

Problems solved by technology

In general, this transformation takes a long time and the resulting gel has poor mechanical properties ([J] Biomacromolecules, 2004, 5(3):786-792), which greatly limits silk fibroin as an excellent biological Therefore, the modification of silk fibroin gel, including physical blending and chemical crosslinking, has become a research hotspot, mainly to improve the mechanical properties and stability of the gel.

But at present there is no silk fibroin gel prepared by a method that can present higher mechanical properties with a simpler process and shorter gelation time.