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A kind of method for preparing heparin sodium by biological enzymatic method

A bio-enzymatic method, a technology of heparin sodium, is applied in the field of bio-enzymatic preparation of heparin sodium, and achieves the effects of high activity yield, unbreakable, and stable adsorption

Active Publication Date: 2016-03-23
ANHUI KEBAO BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many kinds of heparin sodium extraction processes on the market, but most of them are prepared by pig intestines, but the content of heparin in pig lungs is much higher than that in pig intestines. It is now necessary to provide a low-cost and simple process for the preparation of heparin sodium using pig lungs as raw materials. Methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of method that the present invention proposes to prepare heparin sodium by biological enzymatic method comprises the following steps:

[0021] S1. Clean the pig lungs, remove the internal and external dirt and fat, mince them into mince, and add water under sufficient stirring to obtain the pig lung mixture, wherein the mass ratio of pig lungs to water is 1:2;

[0022] S2, adding 0.2mol / L sodium hydroxide solution to the pig lung mixed solution to make the pH reach 9, adding trypsin extracted from 0.8wt% pig pancreas based on the pig lung mixed solution, at room temperature with a stirring speed of 95rpm Stir for 70 minutes to obtain the primary enzymolysis mixture, then raise the primary enzymolysis mixture to 35°C at a heating rate of 3°C / min and stir at a stirring speed of 40rpm for 240min to obtain the second enzymolysis mixture, maintain the liquid during the stirring process The pH value is 8.5;

[0023] S3. Raise the second enzymolysis mixture to 65°C at ...

Embodiment 2

[0032] A kind of method that the present invention proposes to prepare heparin sodium by biological enzymatic method comprises the following steps:

[0033] S1. Clean the pig lungs, remove the internal and external dirt and fat, mince them into mince, and add water to stir to obtain the pig lung mixture, wherein the mass ratio of pig lungs to water is 1:1;

[0034] S2, add 0.5mol / L sodium hydroxide solution to the pig lung mixed solution to make the pH reach 8, add the trypsin extracted from 1wt% pig pancreas based on the pig lung mixed solution, stir at room temperature with a stirring speed of 85rpm 75min to obtain the first enzymolysis mixture, then raise the primary enzymolysis mixture to 39°C at a heating rate of 2°C / min and stir at a stirring speed of 30rpm for 300min to obtain the second enzymolysis mixture, maintain the pH of the liquid during the stirring process value is 7;

[0035] S3. Raise the second enzymolysis mixture to 61°C at a heating rate of 2°C / min and st...

Embodiment 3

[0044] A kind of method that the present invention proposes to prepare heparin sodium by biological enzymatic method comprises the following steps:

[0045] S1. Clean the pig lungs, remove the internal and external dirt and fat, mince them into mince, and add water under full stirring to obtain a pig lung mixture, wherein the mass ratio of pig lungs to water is 1:1.3;

[0046] S2, add 0.4mol / L sodium hydroxide solution to the pig lung mixed solution to make the pH reach 8.7, add the trypsin extracted from 0.9wt% pig pancreas based on the pig lung mixed solution, at room temperature with a stirring speed of 90rpm Stir for 73 minutes to obtain the primary enzymolysis mixture, then raise the primary enzymolysis mixture to 37°C at a heating rate of 2.2°C / min and stir at a stirring speed of 34rpm for 270min to obtain the second enzymolysis mixture, and maintain the liquid during the stirring process The pH value is 7.6;

[0047] S3. Raise the second enzymolysis mixture to 63°C at ...

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Abstract

The invention discloses a method for preparing heparin sodium by a biological enzyme process, which comprises the following steps: pulverizing pig lungs, carrying out enzymolysis, carrying out deproteinization treatment, decolorizing by oxidization, carrying out resin exchange to obtain a heparin sodium solution, precipitating the heparin sodium solution, dehydrating and drying to obtain the heparin sodium fine product. The method has the advantages of wide raw material sources, high universality, high activity and yield, stable technical quality and low production cost, and implements low investment, low cost and high recovery rate of the heparin sodium.

Description

technical field [0001] The invention relates to the field of mucopolysaccharide biopharmaceuticals, in particular to a method for preparing heparin sodium by a biological enzymatic method. Background technique [0002] Heparin Sodium (HeparinSodium) Heparin Sodium is a mucopolysaccharide sulfate anticoagulant. Heparin sodium is the sodium salt of aminodextran sulfate extracted from the mucous membrane of pigs or cattle, and belongs to mucopolysaccharides. Studies in recent years have proved that heparin sodium also has the effect of lowering blood lipids. At present, there are many kinds of heparin sodium extraction processes on the market, but most of them are prepared by pig intestines. However, the heparin content in pig lungs is much higher than that in pig intestines. It is now necessary to provide a low-cost and simple process for the preparation of heparin sodium using pig lungs as raw materials. Methods. Contents of the invention [0003] The technical problems ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/10
Inventor 赵厚发徐宾朋郭庆
Owner ANHUI KEBAO BIOLOGICAL ENG CO LTD
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