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Probiotic agent containing Lactobacillus plantarum and Bifidobacterium lactis and preparation method thereof

A technology of Bifidobacterium lactis and Lactobacillus plantarum, which is applied in the field of feed microorganisms, can solve the problems of complicated operation of machinery and equipment, reduced feed utilization rate, competitive exclusion of bacterial species, etc., and achieves high substrate utilization rate, stable microbial population, The effect of improving the ability to infect

Active Publication Date: 2016-06-15
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to the above patent documents, solid-state fermentation is mostly used in the preparation of fermented feed. Although the solid-state fermentation overcomes the high cost, high environmental pollution, difficult post-processing, and cumbersome operation of machinery and equipment, etc. However, due to the addition of probiotics to mixed fermentation at the same time, if the strains are not properly selected, it is easy to cause competitive exclusion between strains, affect the complete decomposition of beneficial ingredients in fermented products, reduce the utilization rate of feed, and increase costs. And it is not conducive to the absorption of the body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of probiotics containing plantaractobacillus, bifidobacterium lactis, its preparation method comprises the steps:

[0023] Preparation of seed liquid: Inoculate Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9 from frozen glycerol tubes into bottles containing liquid seed medium, and culture them statically at 37°C for 24 hours in an incubator;

[0024] The seed culture medium of Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9 is: peptone 1%, glucose 2%, water added to 100%, pH 7.0-7.2.

[0025] Fermentation culture conditions: add Lactobacillus plantarum HM-10 seed solution to the solid medium for mixing and fermentation, control the fermentation temperature at 36°C, humidity at 50%, initial pH 7.0, add Bifidobacterium lactis V9 after 8 hours of fermentation The seed solution was continued to ferment for 24 hours, the fermentation temperature was controlled to be 34°C, and the humidity was 52%; the free amino nitrogen content was 278umol / g...

Embodiment 2

[0031] A kind of probiotics containing plantaractobacillus, bifidobacterium lactis, its preparation method comprises the steps:

[0032] Preparation of seed solution: culture the seeds connected with Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9 at 37°C for 24 hours in an incubator;

[0033] The seed culture medium of Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9 is: peptone 1%, glucose 2%, water added to 100%, pH 7.0-7.2.

[0034]Fermentation culture conditions: add Lactobacillus plantarum HM-10 seed solution to the solid medium for mixing and fermentation, control the fermentation temperature at 34°C, humidity at 55%, initial pH 7.2, add Bifidobacterium lactis V9 after 12 hours of fermentation The seed solution was continued to ferment for 22 hours, the fermentation temperature was controlled to be 32°C, and the humidity was 55%; the content of free amino nitrogen at the end of the fermentation was 275umol / g;

[0035] The solid fermentation mediu...

Embodiment 3

[0040] A kind of probiotics containing plantaractobacillus, bifidobacterium lactis, its preparation method comprises the steps:

[0041] Preparation of seed solution: culture the seeds connected with Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9 at 37°C for 24 hours in an incubator;

[0042] The seed culture medium of Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9 is: peptone 1%, glucose 2%, water added to 100%, pH 7.0-7.2.

[0043] Fermentation culture conditions: add Lactobacillus plantarum HM-10 seed liquid to solid medium for mixing and fermentation, control the fermentation temperature at 38°C, humidity at 45%, initial pH 6.8, add Bifidobacterium lactis V9 after 6 hours of fermentation The seed solution was continued to ferment for 26 hours, the fermentation temperature was controlled to be 36°C, and the humidity was 50%; the content of free amino nitrogen at the end of the fermentation was 289umol / g;

[0044] The solid fermentation medium is: ...

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PUM

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Abstract

The invention discloses a probiotic agent containing Lactobacillus plantarum and Bifidobacterium lactis, and its preparing method, and belongs to the technical field of feed microbes. The probiotic agent having the advantages of special sour fragrance, high number of contained live bacteria and improved degradation rate of proteins in the soybean meal, wheat bran and corn flour is prepared through the solid-state fermentation of soybean meal, wheat bran and corn flour by adopting Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9; and the method has the characteristics of low production cost and simple operation. The adoption of mixed bacterium segment solid state fermentation can utilize the mutualism synergism of strains, and can improve the yield and the thallus concentration; and the probiotic agent utilizing the growth, propagation and metabolism of the Lactobacillus plantarum HM-10 in the early stage of fermentation can provide abundant nutrients and necessary anaerobic environment for the propagation of the Bifidobacterium lactis V9, so it is helpful for the growth of the Bifidobacterium lactis V9, and the number of Lactobacillus plantarum HM-10 live bacteria is increased.

Description

technical field [0001] The invention belongs to the technical field of feed microorganisms, and relates to a probiotic agent containing plant lactobacillus and lactobacillus lactis prepared by solid-state fermentation technology using soybean meal, bran and corn flour as substrates. Background technique [0002] At present, probiotic products mostly use probiotics to directly ferment with liquid milk and soy milk to obtain functional yogurt or soy milk, and the rest are fermented in liquid state to directly collect probiotic cells and make probiotic powder, tablets or capsules. The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment. Since probiotics can improve the micro-ecological balance of the host, improve the health level of the host and live bacterial preparations and metabolites of the healthy state, the research on probioti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/10C12R1/25C12R1/01A23K10/12
Inventor 赵树平乔向前高鹏飞
Owner INNER MONGOLIA SCI PLUS BIOTECH
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