Feed microecological preparation and preparation method thereof

A microecological preparation and feeding technology, which is applied in the field of feeding microecological preparations and its preparation, can solve the problems of poor stability and repeatability of the effect, and ineffective application effects, etc., and achieve strong palatability, increase daily weight gain, and improve quality effect

Inactive Publication Date: 2014-08-20
凯帕雷森(江苏)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many enterprises in my country have realized the industrialized production of live microbial preparations for feed and applied them in feed, but there are problems such as not outstanding application effect, poor effect stability and repeatability.

Method used

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  • Feed microecological preparation and preparation method thereof
  • Feed microecological preparation and preparation method thereof
  • Feed microecological preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 kind of liquid preparation:

[0021] 1. Source of bacteria

[0022] Lactobacillus acidophilus source: CGMCC, Institute of Microbiology, Chinese Academy of Sciences, Lactobacillus plantarum subspecies, AS1.2707; Bacillus subtilis source: CGMCC, Institute of Microbiology, Chinese Academy of Sciences, Bacillus subtilis Subspecies, AS1.2163; Saccharomyces cerevisiae source: CGMCC, Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Saccharomyces cerevisiae, AS2.3875.

[0023] 2. Saccharomyces cerevisiae

[0024] 2.1 Incline strains: medium: yeast extract 1wt%, peptone 2wt%, glucose 2wt%, agar 2wt%. Sterilize at 0.1Mpa for 20 minutes, inoculate aseptically, and incubate at 28°C for 48 hours.

[0025] 2.2 Erlenmeyer flask shaker culture: medium: glucose 2wt%, urea 0.5wt%, ammonium sulfate 1wt%, yeast extract 0.5wt%. Sterilize at 0.1Mpa for 20 minutes, inoculate aseptically, and culture on a shaker at 28°C for 48 hours, with...

Embodiment 2

[0064] The preparation method of the present embodiment feed probiotics comprises the following steps:

[0065] (1) Strain equipment: equipped with Lactobacillus acidophilus 32.1х10 8 cfu / L, Saccharomyces cerevisiae 1х10 8 cfu / L, Bacillus subtilis 61.5х10 8 cfu / L;

[0066] (2) Preparation of solid medium: by weight percentage, mix the following raw materials evenly: bran 80%, apple pomace 15%, corn flour 2%, brown sugar 2%, dipotassium hydrogen phosphate 0.5% and ammonium sulfate 0.5%; After sterilization, add water to adjust the water content to 12wt%, and pack it into solid material bags (plastic bags with one-way air valves that ventilate outward);

[0067] (3) Inoculation and fermentation: first mix Lactobacillus acidophilus and Saccharomyces cerevisiae at a volume ratio of 1:1, then inoculate the solid medium at an inoculum amount of 30wt% relative to the solid medium, and incubate at 30°C After 7 days of anaerobic fermentation, the inoculation amount of 10wt% rela...

Embodiment 3

[0069] The preparation method of the present embodiment feed probiotics comprises the following steps:

[0070] (1) Strain equipment: equipped with Lactobacillus acidophilus 3х10 9 cfu / L, Saccharomyces cerevisiae not less than 1.1х10 8 cfu / L, Bacillus subtilis not less than 6х10 9 cfu / L;

[0071] (2) Preparation of solid medium: by weight percentage, mix the following raw materials evenly: bran 75%, apple pomace 20%, corn flour 1%, brown sugar 3%, dipotassium hydrogen phosphate 0.2% and ammonium sulfate 0.8%; After sterilization, add water to adjust the water content to 10wt%, and pack it into solid material bags (plastic bags with one-way air valves that ventilate outward);

[0072] (3) Inoculation and fermentation: first mix Lactobacillus acidophilus and Saccharomyces cerevisiae at a volume ratio of 1:0.8, and then inoculate the above solid medium at an inoculation amount of 25wt% relative to the solid medium, at 25°C After 8 days of anaerobic fermentation, the inocul...

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Abstract

The invention belongs to the technical field of biological fermentation and in particular relates to a feed microecological preparation and a preparation method thereof. The preparation method comprises the following steps: (1) preparing lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis; (2) preparing a solid culture medium by uniformly mixing the following raw materials: bran, apple pomace, corn meal, brown sugar, dipotassium phosphate and ammonium sulfate according to certain amounts; sterilizing and adding water for adjusting the content of the water; (3) inoculating twice and fermenting stage by stage. According to the feed microecological preparation, the growth state of a large quantity of microbial strains is good, and a fermentation culture medium is sour and fragrant in taste, good in palatability and favorable for being used in a feed. According to the preparation method, the process of inoculating twice and fermenting stage by stage is adopted and good material conditions and growth environments are provided.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a feed microecological preparation and a preparation method thereof. Background technique [0002] The research and application of animal microecological preparations can be traced back to 1947. Mollgaard first discovered that feeding piglets with Lactobacillus can effectively increase body weight and improve health. However, the middle and latter part of the last century was the golden age of antibiotic research, production and use, which made the research and development of probiotics at a low ebb. In recent years, due to the extensive use of antibiotics and synthetic drugs in the aquaculture industry, the safety hazards of animal-sourced food and environmental pollution have been caused, causing serious social, economic and ecological problems, and microecological preparations have become a research hotspot again. [0003] Feed probiotics are probi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16A23K1/14A23K1/175A23K1/18A23K10/12A23K10/30A23K10/37A23K20/163A23K20/26A23K50/30
CPCY02P60/87
Inventor 杨建平赵志军刘永祥刘延贺胡华锋
Owner 凯帕雷森(江苏)生物科技有限公司
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