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Preventive and therapeutic effect of quercetin to diabetic cataract

A technology of diabetes and quercetin, which is applied in the field of cataract prevention and treatment, can solve the problems that have not been reported in the research, and achieve the effect of improving protein expression level, strong effect and good medicinal prospect

Inactive Publication Date: 2014-08-27
XUZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its research in diabetic cataract has not been reported

Method used

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  • Preventive and therapeutic effect of quercetin to diabetic cataract
  • Preventive and therapeutic effect of quercetin to diabetic cataract
  • Preventive and therapeutic effect of quercetin to diabetic cataract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Effect of quercetin on the overall physiological condition, blood sugar and body weight of DC rats;

[0034] 1. Materials and Methods

[0035] 1.1 Experimental animals Adult healthy male SD rats, weighing 150-190 g, were provided by the Experimental Animal Center of Xuzhou Medical College.

[0036] 1.2 Drugs and reagents Quercetin was purchased from Shaanxi Huike Plant Development Co., Ltd. (purity 98%), pirenoxine sodium was purchased from Wuhan Wujing Co., Ltd., streptozotocin was purchased from Sigma Company of the United States, and blood glucose testing kit was purchased from The Institute of Bioengineering was established in Nanjing.

[0037] 1.3 Experimental method

[0038] 1.3.1 Modeling and experimental treatment

[0039] 53 male SD rats, with a body weight of 165.7±10.2g, no lens abnormalities were detected by mydriasis, and 8 were randomly selected as the normal group. After the rest of the rats were fasted for 24 hours, they were intraperito...

Embodiment 2

[0057] Example 2: Effect of Quercetin on Lens Opacity of Diabetic Cataract Rats

[0058] 1. Materials and Methods

[0059] 1.1 Experimental animals, medicines and reagents are the same as in Example 1.

[0060] 1.2 Observation of lens opacity

[0061] One drop of tropicamide eye drops was given to both eyes of the rats, and the changes of the lens were observed with a slit lamp 5 minutes later. The changes in the shape of the lens of the rat were observed and recorded, and the degree of lens turbidity was divided into grades Ⅰ-Ⅴ according to the standard of Azuma et al. . Grade Ⅰ: no turbidity, transparent crystal; Grade Ⅱ: mild turbidity, vacuoles appear around the crystal; The peripheral vacuoles extend to the nuclear area, and the nuclear haze is aggravated; Grade V: nuclear opacity, and the cataract is mature.

[0062] 1.3 Statistical processing

[0063] Rank data were analyzed using the nonparametric rank sum test.

[0064] 2. Results

[0065] 2.1 Effect of quercet...

Embodiment 3

[0070] Example 3: Effect of Quercetin on Opacity of Lens Tissue Cultured in High Glucose

[0071] 1. Materials and Methods

[0072] 1.1 In vitro lens tissue culture

[0073] 30 male SD rats, weighing 150-190g, were sacrificed after mydriasis examination without lens abnormalities, and the eyeballs were quickly removed at low temperature, and the fascia tissue around the eyeballs was removed, and then the eyeballs were placed in the normal saline solution of penicillin and streptomycin , rinsed with PBS solution for 3 times under sterile conditions, cut the eyeball wall circularly from 3-4mm behind the limbus, then cut the suspensory ligament of the lens circularly, rinsed with PBS solution for 3 times, put the taken out lens into 2.5mL M199 culture solution (100mL / LL-glutamine, 2.6mmol / L fructose, penicillin 10 5 U / L, streptomycin 0.1g / L) in a 24-well culture plate at 37°C containing 5% CO 2 After culturing in the incubator for 24 hours, the turbid lens due to operation dam...

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Abstract

The invention relates to novel pharmacologic action of quercetin, which means the preventive and therapeutic effect of quercetin to diabetic cataract. Quercetin remarkably improves phacoscotasmus induced by diabetes, remarkably reduces the level of advanced glycation end products (AGEs) and the activity of aldose reductase (AR) in the crystalline lens, remarkably improves the activity of total superoxide dismutase (T-SOD) and the level of reduced glutathione (GSH) in the crystalline lens, improves the oxidation resistance of the crystalline lens, remarkably reduces the expression level of alpha smooth muscle actin (alpha-SMA) in the lens epithelial cells, improves the protein level of E-cadherin, blocks the signal channel of TGF-beta2 / Akt, inhibits transdifferentiation of the lens epithelial cells and the like. The quercetin can be used for preventing and treating diabetic complication such as diabetic cataract.

Description

technical field [0001] The invention belongs to the technical field of cataract prevention and treatment, and relates to the prevention and treatment effect of quercetin on diabetic cataract. Background technique [0002] Diabetic cataract (DC) refers to that under the stimulation of long-term high blood sugar, the patient's lens appears cloudy to varying degrees, which eventually leads to vision loss, damage, and even blindness. According to the statistics of the International Diabetes Federation (IDF), in the 1990s, there were approximately 100 million people with diabetes worldwide, and the number will increase to 552 million by 2030. With the increasing number of type 1 and type 2 diabetes patients worldwide, the incidence of diabetic cataract is gradually increasing. According to statistics, the incidence of diabetic cataract in diabetic patients is 60%-65%, and it has become the second major eye disease among diabetic complications after retinopathy. The commonly use...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P27/12
Inventor 印晓星鲁茜杜蕾杨婷婷张楠刘耀武张帆
Owner XUZHOU MEDICAL COLLEGE
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