Preventive and therapeutic effect of quercetin to diabetic cataract

A technology of diabetes and quercetin, which is applied in the field of cataract prevention and treatment, can solve the problems that have not been reported in the research, and achieve the effect of improving protein expression level, strong effect and good medicinal prospect

Inactive Publication Date: 2014-08-27
XUZHOU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its research in diabeti

Method used

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  • Preventive and therapeutic effect of quercetin to diabetic cataract
  • Preventive and therapeutic effect of quercetin to diabetic cataract
  • Preventive and therapeutic effect of quercetin to diabetic cataract

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1: The effect of quercetin on the overall physiological condition, blood sugar and body weight of DC rats;

[0034] 1. Materials and methods

[0035] 1.1 Experimental animals Adult healthy male SD rats weighing 150-190g were provided by the Experimental Animal Center of Xuzhou Medical College.

[0036] 1.2 Drugs and reagents Quercetin was purchased from Shaanxi Huike Plant Development Co., Ltd. (purity 98%), pirenoxine sodium was purchased from Wuhan Wujing Co., Ltd., streptozotocin was purchased from Sigma Company, USA, and blood glucose test kit was purchased Since the establishment of Nanjing Institute of Bioengineering.

[0037] 1.3 Experimental method

[0038] 1.3.1 Modeling and experimental treatment

[0039] There were 53 male SD rats weighing 165.7±10.2g. After mydriasis examination without lens abnormalities, 8 were randomly selected as the normal group. The rest of the rats were fasted for 24 hours and injected STZ65mg / kg into the abdominal cavity at a time (bef...

Example Embodiment

[0057] Example 2: Effect of Quercetin on Lens Opacity in Rats with Diabetic Cataract

[0058] 1. Materials and methods

[0059] 1.1 Experimental animals, drugs and reagents are the same as in Example 1.

[0060] 1.2 Observation of lens opacity

[0061] Rats were given 1 drop of tropikamide eye drops to both eyes, and 5 minutes later, the lens changes were observed using a slit lamp, and the changes in the morphology of the rat’s lens were observed and recorded. The degree of lens opacity was divided into grades Ⅰ~Ⅴ according to the standards of Azuma. . Grade Ⅰ: no turbidity, transparent crystal; Grade Ⅱ: mild turbidity, cavitation around the crystal; Grade Ⅲ: moderate turbidity, cavitation around the crystal expands to the center, foggy turbidity in the nucleus; Grade IV: high turbidity, crystal Peripheral vacuoles extend to the nuclear area, and nuclear foggy opacity is aggravated; Grade V: nuclear opacity and mature cataract.

[0062] 1.3 Statistical processing

[0063] Grade data ...

Example Embodiment

[0070] Example 3: The effect of quercetin on the turbidity of lens tissue cultured with high glucose

[0071] 1. Materials and methods

[0072] 1.1 Isolated lens tissue culture

[0073] Thirty male SD rats weighing 150-190g were sacrificed after mydriatic examination without lens abnormalities. The eyeballs were quickly removed at low temperature, the fascia tissue around the eyeballs was removed, and the eyeballs were placed in penicillin-streptomycin saline. , Rinse 3 times with PBS solution under aseptic conditions, cut the eyeball wall circularly from 3-4mm behind the limbus, then cut the suspensory ligament circularly, rinse 3 times with PBS solution, put the removed lens into 2.5ml LM199 culture medium (100mL / LL-glutamine, 2.6mmol / L fructose, penicillin 10 5 U / L, streptomycin 0.1g / L) in a 24-well culture plate, placed at 37℃, containing 5% CO 2 After 24 hours of incubation in the incubator, the lens that was cloudy due to operation damage was discarded, and the transparent len...

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Abstract

The invention relates to novel pharmacologic action of quercetin, which means the preventive and therapeutic effect of quercetin to diabetic cataract. Quercetin remarkably improves phacoscotasmus induced by diabetes, remarkably reduces the level of advanced glycation end products (AGEs) and the activity of aldose reductase (AR) in the crystalline lens, remarkably improves the activity of total superoxide dismutase (T-SOD) and the level of reduced glutathione (GSH) in the crystalline lens, improves the oxidation resistance of the crystalline lens, remarkably reduces the expression level of alpha smooth muscle actin (alpha-SMA) in the lens epithelial cells, improves the protein level of E-cadherin, blocks the signal channel of TGF-beta2/Akt, inhibits transdifferentiation of the lens epithelial cells and the like. The quercetin can be used for preventing and treating diabetic complication such as diabetic cataract.

Description

technical field [0001] The invention belongs to the technical field of cataract prevention and treatment, and relates to the prevention and treatment effect of quercetin on diabetic cataract. Background technique [0002] Diabetic cataract (DC) refers to that under the stimulation of long-term high blood sugar, the patient's lens appears cloudy to varying degrees, which eventually leads to vision loss, damage, and even blindness. According to the statistics of the International Diabetes Federation (IDF), in the 1990s, there were approximately 100 million people with diabetes worldwide, and the number will increase to 552 million by 2030. With the increasing number of type 1 and type 2 diabetes patients worldwide, the incidence of diabetic cataract is gradually increasing. According to statistics, the incidence of diabetic cataract in diabetic patients is 60%-65%, and it has become the second major eye disease among diabetic complications after retinopathy. The commonly use...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P27/12
Inventor 印晓星鲁茜杜蕾杨婷婷张楠刘耀武张帆
Owner XUZHOU MEDICAL COLLEGE
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