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A method for enzymatic hydrolysis to prepare Cynomorium polysaccharide

A technology of Cynomorium polysaccharide and Cynomorium polysaccharide, which is applied in the field of Cynomorium polysaccharide preparation and enzymatic hydrolysis to achieve good decolorization effect, simple process and low extraction cost

Active Publication Date: 2016-05-04
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commercially available Cynomorium polysaccharides are mostly brown or brown-red powders, containing a large amount of tannins and anthocyanins, and their color seriously affects the application of Cynomorium polysaccharide formulations. It is necessary to invent a polysaccharide with high extraction rate and high polysaccharide yield, and Preparation method of Cynomorium polysaccharide with good decolorization effect and beneficial to the application of various dosage forms

Method used

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  • A method for enzymatic hydrolysis to prepare Cynomorium polysaccharide
  • A method for enzymatic hydrolysis to prepare Cynomorium polysaccharide
  • A method for enzymatic hydrolysis to prepare Cynomorium polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1, pepsin assisted enzymatic hydrolysis to prepare Cynomorium polysaccharide

[0051] Weigh 2.00 g of degreased and dried Cynomorium powder into a stoppered Erlenmeyer flask, add 40.0 mg of pepsin according to the enzyme dosage of 2% (mass percentage), and add 100 ml of pH 2.0 according to the ratio of material to liquid (W / V) 1 g: 50 ml Hydrochloric acid aqueous solution, enzymolysis at 40°C for 5 hours, keeping pepsin at low boiling reflux for 1 hour to inactivate pepsin, left to cool and then centrifuged (5000r / min, 20min, 4°C), the supernatant was prepared according to the Sevage method (chloroform: n-butanol volume Ratio 4:1) to remove protein for 3 times, discard the lower organic phase, concentrate the upper aqueous solution to a small volume by rotary evaporation under reduced pressure, add 4 times the volume of anhydrous ethanol to precipitate, stand overnight in the refrigerator at 4°C, and centrifuge (5000r / min, 20min, 4°C), the resulting precipit...

Embodiment 2

[0054] Embodiment 2, pepsin assisted enzymatic hydrolysis to prepare Cynomorium polysaccharide

[0055] Weigh 2.00g of degreased and dried Cynomorium powder into a stoppered Erlenmeyer flask, add 40.0mg of pepsin according to the enzyme dosage of 2% (mass percentage), and add 100ml of pH1.5 according to the ratio of material to liquid (W / V) 1g: 50ml Hydrochloric acid aqueous solution, enzymolysis at 40°C for 5 hours, keeping pepsin at low boiling reflux for 1 hour to inactivate pepsin, left to cool and then centrifuged (5000r / min, 20min, 4°C), the supernatant was prepared according to the Sevage method (chloroform: n-butanol volume Ratio 4:1) to remove protein for 3 times, discard the lower organic phase, concentrate the upper aqueous solution to a small volume by rotary evaporation under reduced pressure, add 4 times the volume of anhydrous ethanol to precipitate, stand overnight in the refrigerator at 4°C, and centrifuge (5000r / min, 20min, 4°C), the resulting precipitate wa...

Embodiment 3

[0058] Embodiment 3, pepsin assisted enzymatic hydrolysis to prepare Cynomorium polysaccharide

[0059] Weigh 2.00g of degreased and dried Cynomorium powder into a stoppered Erlenmeyer flask, add 30.0mg of pepsin according to the enzyme dosage of 1.5% (mass percentage), and add 100ml of pH1.3 according to the ratio of material to liquid (W / V) 1g: 50ml Hydrochloric acid aqueous solution, enzymolysis at 40°C for 5 hours, keeping pepsin at low boiling reflux for 1 hour to inactivate pepsin, left to cool and then centrifuged (5000r / min, 20min, 4°C), the supernatant was prepared according to the Sevage method (chloroform: n-butanol volume Ratio 4:1) to remove protein for 3 times, discard the lower organic phase, concentrate the upper aqueous solution to a small volume by rotary evaporation under reduced pressure, add 4 times the volume of anhydrous ethanol to precipitate, stand overnight in the refrigerator at 4°C, and centrifuge (5000r / min, 20min, 4°C), the resulting precipitate ...

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Abstract

The invention discloses a method for preparing cynomorium songaricum polysaccharides by virtue of enzymolysis. The invention discloses a method for preparing cynomorium songaricum polysaccharides by virtue of enzymolysis. The method comprises the following steps: adding pepsase into defatted cynomorium songaricum powder so as to obtain mixed powder, wherein the pepsase accounts for 0.5-3wt% of the defatted cynomorium songaricum powder; adding a hydrochloric acid aqueous solution into the mixed powder, and carrying out enzymolysis on the obtained mixture so as to obtain enzymatic hydrolysate; carrying out micro-boiling reflux on the enzymatic hydrolysate, so that the pepsase in the enzymatic hydrolysate is inactivated; cooling and centrifugalizing the obtained product so as to obtain a liquid supernatant; carrying out protein removal on the liquid supernatant by using a Sevage method, and discarding a lower organic phase, so that an upper aqueous solution is obtained; adding anhydrous ethyl alcohol into the upper aqueous solution to carry out alcohol precipitation, so that cynomorium songaricum polysaccharides are obtained. The method disclosed by the invention is simple in process, low in extraction cost, and suitable for industrial mass production, and because compared with traditional processes, cynomorium songaricum polysaccharides prepared by using the preparation method are good in decoloring effect, and light pink in color, thereby laying a foundation for the development of new products with high impression requirements.

Description

technical field [0001] The invention relates to a method for preparing Cynomorium polysaccharide, in particular to a method for preparing Cynomorium polysaccharide by enzymatic hydrolysis, which belongs to the field of traditional Chinese medicine preparation. Background technique [0002] Cynomorium is a root parasitic succulent herb seed plant, commonly known as elixir, and its dried succulent stems can be used as traditional Chinese medicine, Mongolian medicine, and Uighur medicine. The Pharmacopoeia of the People's Republic of China, 2010 Edition, records that Cynomorium is used to nourish kidney yang, benefit essence and blood, moisten intestines and laxative, and mainly treat deficiency of kidney yang, deficiency of essence and blood, weakness of waist and knees, impotence and slippery essence, intestinal dryness and constipation. Modern studies have found that Cynomorium water extract has important medical value in terms of anti-oxidation, anti-hypoxia, anti-epilepsy,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 陈贵林樊海燕于春洋
Owner INNER MONGOLIA UNIVERSITY