Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Centrifugal sedimentation assisted plant transgenic method

A centrifugal sedimentation and transgene technology, applied in the biological field, can solve the problems of increasing the degree of trauma on the surface of the receptor material, and the effect of cells inside the receptor is minimal, so as to improve the efficiency of plant transgene and increase the area of ​​trauma

Inactive Publication Date: 2016-11-23
SHANGHAI JIAOTONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In response to this problem, someone proposed the idea of ​​ultrasonic-assisted transformation of Agrobacterium. This treatment can increase the degree of trauma on the surface of the receptor material, but has little effect on the cells inside the receptor.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The embodiments of the present invention are described in detail below: the present embodiment is implemented under the premise of the technical solution of the present invention, and detailed implementation and specific operation process are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0017] Tobacco for the following examples was obtained from the applicant's laboratory.

[0018] This embodiment includes the following steps:

[0019] Step 1, taking tender leaves from sterile tobacco tissue culture seedlings, cutting off the leaf margins as receptor materials;

[0020] Step 2, activate the Agrobacterium TG1 containing the GUS gene to OD value = 0.6, centrifuge at 50,000 rpm for 5 minutes, and then resuspend the gene with MS liquid culture;

[0021] Step 3, soaking the tobacco recipient leaves in step 1 into the Agrobacterium resuspension, and centrifuging at 5000rpm for 10min;

[0022] Step 4, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A centrifugal sedimentation-assisted plant transgenic method is as follows: the plant receptor material is mixed with the agrobacterium, and the centrifugal sedimentation treatment is carried out at a reasonable rotational speed, and finally a positive transformant is obtained. Centrifugal sedimentation can not only increase the wound area of ​​the receptor material, but also allow Agrobacterium to penetrate into the interior of the receptor material under the action of centrifugal force, which significantly improves the efficiency of plant transgenesis.

Description

technical field [0001] The invention relates to a biotechnology, in particular to a method for centrifugal sedimentation to assist plant transgene. Background technique [0002] As the core of biotechnology, transgenic technology has great development potential in plant improvement, medicine, animal husbandry and food industry. However, the plant transgenic technology based on Agrobacterium transformation currently has the problem of low transformation efficiency, and the transformation rate of most plants is about 1%. This problem has also become the main factor limiting the industrial production of transgenic plants. Studies have shown that when Agrobacterium soaks recipient plant material, only a small number of wounded cells on the surface are likely to be transformed, while most of the inner cells in the recipient material have no opportunity to come into contact with Agrobacterium. In response to this problem, someone proposed the idea of ​​ultrasonic-assisted transfo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84A01H5/00
Inventor 王贵荣李斌连唐克轩
Owner SHANGHAI JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com