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Application of compound cytokines of human umbilical cord mesenchymal stem cells in the preparation of biological preparations for repairing skin damage

A technology of stem cells and cytokines, applied in the field of medicine, can solve problems such as increase and improper treatment selection, and achieve the effect of promoting growth and healing and regulating immune response.

Active Publication Date: 2016-06-15
徐妍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of postoperative complications caused by improper treatment selection and improper operation is increasing year by year.

Method used

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  • Application of compound cytokines of human umbilical cord mesenchymal stem cells in the preparation of biological preparations for repairing skin damage
  • Application of compound cytokines of human umbilical cord mesenchymal stem cells in the preparation of biological preparations for repairing skin damage
  • Application of compound cytokines of human umbilical cord mesenchymal stem cells in the preparation of biological preparations for repairing skin damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of composite cytokines containing human umbilical cord mesenchymal stem cells

[0031] A method for preparing a compound cytokine containing human umbilical cord mesenchymal stem cells, comprising the steps of:

[0032] 1) Extraction of umbilical cord mesenchymal stem cells

[0033] The human umbilical cord tissue obtained through liposuction and other operations is used as raw material, the umbilical cord mesenchymal stem cells are extracted by centrifugation after collagenase digestion, and the umbilical cord mesenchymal stem cells are inoculated in a culture dish at a saturated humidity of 5% carbon dioxide concentration, 37° C cultured at constant temperature.

[0034] 2) Expansion and culture of human umbilical cord mesenchymal stem cells

[0035] Human umbilical cord mesenchymal stem cells are long-spindle-shaped adherent culture cells, which are cultured when the cells grow to 80-90% confluent

[0036] Subculture.

[0037] The morphology...

Embodiment 2

[0044] 19 volunteers aged 24-33 years old with Fitzpatrick skin grade 3-4 were selected to participate in the experiment. Carbon dioxide laser irradiation was applied to four places on the inner sides of each volunteer's arms, and the radiation was measured with 8mJ and 16mJ for the symmetrical areas. figure 2 It is a typical picture after irradiation, T is the experimental group, C is the control group, 1 is 8mJ radiation, and 2 is 16mJ radiation, the pictures are respectively 1, 7, and 14 days after surgery.

[0045] The erythema index and melanin index were counted separately, T is the experimental group, C is the control group, 1 is 8mJ radiation, 2 is 16mJ radiation, respectively 0, 1, 4, 7, 14, 21 days after operation.

[0046] Erythema index was used to measure recovery among different groups (T: experimental group; C: control group) after carbon dioxide laser. T1 and C1 are 8mJ lasers, and T2 and C2 are 16mJ lasers. Such as Figure 3a As shown, there was a significa...

Embodiment 3

[0049] Example 3 Functional Repair Material Containing Human Umbilical Cord Mesenchymal Stem Cell Compound Cytokines

[0050] Functional repair material: It can be applied to the repair of skin wounds such as laser burns, acute trauma, contusion, etc. It contains high doses of cytokines and has a very strong effect on the repair of fresh or old unclosed wounds. 10ml repair material contains: HA0.1g, ultrapure water about 10ml, human umbilical cord mesenchymal stem cell compound cytokine 100mg, basic cell growth factor 100ng, epidermal growth factor 10μg. The preparation form is a gel-like smear repair material, which can be directly applied to skin wounds, and a breathable material is pasted on the surface to prevent loss of the repair material.

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Abstract

The invention provides the application of human umbilical cord mesenchymal stem cell compound cytokines in the preparation of biological preparations for repairing skin damage. The skin damage includes laser burns, thermal burns, freezing injuries, or physical and chemical damage to the skin from common trauma . In the present invention, for the first time, the compound growth factor of umbilical cord stem cells is used in the treatment of human skin laser damage. Under certain storage conditions, the compound cytokines of human umbilical cord mesenchymal stem cells can preserve the maximum activity and exert the maximum damage repairing effect.

Description

technical field [0001] The invention relates to the application of human umbilical cord mesenchymal stem cell compound cytokines in the preparation of biological preparations for repairing skin damage, and belongs to the technical field of medicine. Background technique [0002] Human umbilical cord mesenchymal stem cells are a kind of mesenchymal cells derived from human umbilical cord tissue, which have high proliferation, self-renewal and multidirectional differentiation capabilities. Mesenchymal stem cells come from a wide range of sources, including bone marrow, fat, umbilical cord blood, placenta, amniotic fluid, umbilical cord and other tissues. Different induction media can be used for in vitro culture to differentiate into cartilage, bone, skeletal muscle, tendon, fat, nerve, skin and other cells. In addition, mesenchymal stem cells have the characteristics of adherent growth, which facilitates the collection of conditioned medium. [0003] Compared with other sou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/19A61P17/00
Inventor 徐妍郭世磊周炳荣
Owner 徐妍
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