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Method for preparing resting cells in process of preparing ethanol by utilizing anaerobic fermentation of synthesis gas

A technology for anaerobic fermentation and ethanol production, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as the preparation method of microbial resting cells that have not yet been seen, and achieve improved conversion efficiency and good energy. Metabolism, overcoming the effects of disturbances

Active Publication Date: 2014-09-10
HENAN AGRICULTURAL UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, there is no suitable preparation method for microbial quiescent cells in the process of producing ethanol by anaerobic fermentation of syngas by microorganisms.

Method used

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  • Method for preparing resting cells in process of preparing ethanol by utilizing anaerobic fermentation of synthesis gas

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Experimental program
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Embodiment

[0032] to prepare Clostridium autoethanogenum Resting cells of DSM10061 are taken as an example, and the specific preparation method includes the following steps:

[0033] (1) Experimental strains Clostridium autoethanogenum DSM10061 was purchased from the German Culture Collection, and the purchased strains were activated and enriched and grown under anaerobic conditions in an anaerobic box; during the enrichment and growth process, samples were taken every 12 hours, and the samples were measured at OD with a UV spectrophotometer. 600 Measure at the wavelength, and proceed to the next step after the growth is stable; after about 96 hours of enrichment culture, the microbial cells enter the growth stable period, and the biomass is stable and no longer grows;

[0034] The activation of the strains uses an activation medium, and the activation medium is a liquid medium, taking 1L capacity as an example, the formula is as follows: NH 4 Cl 0.7~1.2g, NaCl 0.7~1.2g, MgCl 2 0...

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Abstract

The invention belongs to the technical field of preparing ethanol by anaerobically fermenting synthesis gas by virtue of microbes and particularly relates to a method for preparing resting cells of microbes in the process of preparing ethanol by anaerobically fermenting synthesis gas by virtue of microbes. The method comprises the following steps: activating microbial strains; enriching and growing under anaerobic condition; centrifuging; collecting cell precipitates; performing resuspension culturing and the like. According to the preparation method, a culture medium can overcome interference caused by cell growth in the cell liquid culturing process by specially optimizing, the growing phases of anaerobic microbial resting cells are consistent, and the cells can better perform energy metabolism, so that the efficiency of conversion from converting synthesis gas into ethanol can be improved, and the industrialization of convertion from synthesis gas to ethanol can be promoted.

Description

technical field [0001] The invention belongs to the technical field of producing ethanol by anaerobic fermentation of synthesis gas by using microorganisms, and in particular relates to a preparation method of resting cells of microorganisms in the process of producing ethanol by anaerobic fermentation of synthesis gas by microorganisms. Background technique [0002] Energy is an important material basis for human survival and development, and it is also the focus of international politics, economy, military and diplomacy. The impact of energy consumption on the ecological environment is becoming more and more prominent. To solve the energy problem, we must not only pay attention to the balance between supply and demand, but also pay attention to the ecological and environmental problems caused by it. [0003] Ethanol is a high-quality liquid fuel that can be used alone or added to gasoline as a clean energy source. In the prior art, the use of grain raw materials to obtain...

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Application Information

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IPC IPC(8): C12N1/20C12P7/06C12R1/145
CPCY02E50/17Y02E50/10
Inventor 宋安东杨大娇王风芹谢慧张炎达
Owner HENAN AGRICULTURAL UNIVERSITY