A method for screening and domesticating polyhydroxyalkanoate-producing bacteria utilizing xylose

Abstract

The invention provides a method for screening and acclimating polyhydroxyalkanoate-producing bacteria using xylose, and obtains the mixed bacteria using xylose to produce PHA in one step. Referring to the domestication mode of fullness and starvation of the PHA synthetic flora, the sludge samples were domesticated with xylose as the only carbon source. During the domestication process, the Nile blue staining method of the polyhydroxyalkanoate synthetic flora was screened and reinjected into wood. in sugar-acclimated flora. Finally, a stable and high-yield mixed flora of PHA was obtained, and there were mainly four dominant strains of γ-Proteobacteria, Cellvibrio sp., Uncultured bacterium and Pseudomonas putida in the mixed flora system. The method is simple and effective, the domestication time is short, and the obtained bacterial group has a strong ability to use xylose to produce PHA, which is of great significance and practical value for realizing the fermentation of cellulose-based raw materials to produce PHA.

Description

technical field [0001] The invention relates to a screening and acclimation method for efficiently utilizing xylose to produce polyhydroxyalkanoate flora. Background technique [0002] Polyhydroxyalkanoates (polyhydroxyalkanoates PHA) are carbon source storage particles synthesized by many bacteria under unbalanced growth conditions, and are favored as a renewable and biodegradable plastic. In addition to its properties similar to traditional plastics, it also has the characteristics of biocompatibility and optical activity. It has become a good substitute for plastics and has great potential in solving environmental pollution and energy shortages. At present, PHA is mainly produced by fermentation of pure bacteria, including Alcaligenes, nitrogen-fixing bacteria, Pseudomonas and recombinant Escherichia coli. However, pure bacterial fermentation requires the use of high-quality substrates such as glucose, as well as fermentation control processes such as sterilization, resu...

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Problems solved by technology

However, there is a lack of suitable domestication and screening pressure for PHA synthetic strains, and the traditional satiety-starvation mode and anaerobic aerobic mode have a long domestication period, and require small molecular fatty acids as domestication substrates, so there is currently no use of mixed flora A report on the production of PHA from xylose

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