Bifidobacteria longum and application thereof, and functional food composition and preparation method thereof
A Bifidobacterium longum, functional food technology, applied in food preparation, biochemical equipment and methods, microorganism-based methods, etc., can solve the problems of side effects, single material source, high cost of separation and purification, and achieve the effect of delaying aging
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Embodiment 11
[0046] This example is used to illustrate the anti-aging effect of Bifidobacterium longum of the present invention.
[0047] (1) Inoculate Escherichia coli OP50 into LB liquid medium with 1% inoculum amount, shake and culture in a constant temperature shaker at 37°C and 220rpm for 8 hours to the end of logarithm, and obtain Escherichia coli OP50 culture medium; inoculate Bifidobacterium longum with 1% The inoculum amount was inoculated into MRS liquid medium, and cultured in nitrogen-filled anaerobic bottle at 37°C for 12 hours to the end of logarithmic phase to obtain Bifidobacterium longum culture medium.
[0048] Centrifuge the above-mentioned Escherichia coli OP50 and Bifidobacterium longum culture solution at 4000×g for 15 minutes to collect the bacterial cells, wash twice with M9 buffer, then centrifuge at 16000×g at 4°C for 15 minutes, remove all the supernatant, Weigh the wet weight of the bacteria. Resuspend the bacteria with a certain amount of M9 buffer to make the...
Embodiment 12
[0059] This example is used to illustrate the anti-aging effect of Bifidobacterium longum of the present invention.
[0060] (1) Inoculate Escherichia coli OP50 into LB liquid medium with 1% inoculum amount, shake and culture in a constant temperature shaker at 37°C and 220rpm for 8 hours to the end of logarithm, and obtain Escherichia coli OP50 culture medium; inoculate Bifidobacterium longum with 1% The inoculum amount was inoculated into MRS liquid medium, and cultured in nitrogen-filled anaerobic bottle at 37°C for 12 hours to the end of logarithmic phase to obtain Bifidobacterium longum culture medium.
[0061] Centrifuge the above-mentioned Escherichia coli OP50 and Bifidobacterium longum culture solution at 4000×g for 15 minutes to collect the bacterial cells, wash twice with M9 buffer, then centrifuge at 16000×g at 4°C for 15 minutes, remove all the supernatant, Weigh the wet weight of the bacteria. Resuspend the bacteria with a certain amount of M9 buffer to make the...
Embodiment 13
[0072] This example is used to illustrate the anti-aging effect of Bifidobacterium longum of the present invention.
[0073] (1) Inoculate Escherichia coli OP50 into LB liquid medium with 1% inoculum amount, shake and culture in a constant temperature shaker at 37°C and 220rpm for 8 hours to the end of logarithm, and obtain Escherichia coli OP50 culture medium; inoculate Bifidobacterium longum with 1% The inoculum amount was inoculated into MRS liquid medium, and cultured in nitrogen-filled anaerobic bottle at 37°C for 12 hours to the end of logarithmic phase to obtain Bifidobacterium longum culture medium.
[0074] Centrifuge the above-mentioned Escherichia coli OP50 and Bifidobacterium longum culture solution at 4000×g for 15 minutes to collect the bacterial cells, wash twice with M9 buffer, then centrifuge at 16000×g at 4°C for 15 minutes, remove all the supernatant, Weigh the wet weight of the bacteria. Resuspend the bacteria with a certain amount of M9 buffer to make the...
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