Preparation method and application of biological medium obtained by transforming rapeseed cake with Neurospora

A technology for rapeseed meal and culture medium, which is applied to the preparation of biological culture medium obtained by transforming rapeseed meal by Neurospora and its application field, and achieves the effects of reducing environmental pollution, cost and raw material cost.

Active Publication Date: 2016-08-24
OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This medium is a low-cost, high-density excellent medium for fermenting Cryptidinosa to produce DHA, which has not been reported so far.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of biological medium obtained by transforming rapeseed cake with Neurospora
  • Preparation method and application of biological medium obtained by transforming rapeseed cake with Neurospora
  • Preparation method and application of biological medium obtained by transforming rapeseed cake with Neurospora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Using Neurospora crassa as the cake-degrading bacteria, and using its degraded hydrolyzate to cultivate marine microalgae-Cryptidium, follow the steps below:

[0034] A. Preparation of biological medium (hydrolyzate)

[0035] 1. After crushing the rapeseed cake pulverizer left after the oil extraction in the oil factory, weigh 500g of the pulverized rapeseed cake and put it into a disposable fresh-keeping bag, add 1000ml of water and mix well, tie the mouth, 105° C., sterilized for 30 minutes and set aside to obtain a sterilized rapeseed cake.

[0036] 2. Inoculate Neurospora crassa (Neurospora crassa) on the plate of the bran solid medium, and cultivate it for 3-5 days in a 28°C incubator; the composition of the bran solid medium is: bran: agar agar powder: The volume ratio of water = 5:1.5:100.

[0037] 3. Add 5 ml of 0.1% Tween 20 aqueous solution (mass volume ratio, Tween 20: water = 0.1 g: 100 ml) to the cultured Neurospora crassa plate, and add sterilized glass ...

Embodiment 2

[0066] Using Neurospora crassa as the cake-degrading bacteria, use its degradation hydrolyzate to cultivate the marine microalga Cryptidinosa, and follow the steps below:

[0067] A. Preparation of biological medium (hydrolyzate)

[0068] 1. After crushing the rapeseed cake pulverizer left after the oil extraction in the oil factory, weigh 500g of rapeseed cake and put it into a disposable fresh-keeping bag, add 1000ml of water and mix well, tie the mouth, 105℃, Sterilize for 30 minutes and set aside.

[0069] 2. Inoculate Neurospora crassa (Neurospora crassa) on the plate of the bran solid medium, and cultivate it for 3-5 days in a 28°C incubator; the composition of the bran solid medium is: bran: agar agar powder: The volume ratio of water = 5:1.5:100.

[0070] 3. Add 5ml of 0.1% Tween 20 aqueous solution (mass volume ratio, Tween 20: water = 0.1g: 100ml) to the cultured Neurospora crassa plate, and add sterilized glass beads (such as 10 ), gently shaking for a while, the...

Embodiment 3

[0085] A. Preparation of biological medium (hydrolyzate)

[0086] Using Neurospora crassa as the cake-degrading bacteria, use its degradation hydrolyzate to cultivate the marine microalga Cryptidinosa, and follow the steps below:

[0087] 1. After crushing the rapeseed cake pulverizer left after the oil extraction in the oil factory, weigh 500g of rapeseed cake and put it into a disposable fresh-keeping bag, add 750ml of water and mix well, tie the mouth, 105℃, Sterilize for 30 minutes and set aside.

[0088] 2. Inoculate Neurospora crassa (Neurospora crassa) on the plate of the bran solid medium, and cultivate it for 3-5 days in a 28°C incubator; the composition of the bran solid medium is: bran: agar agar powder: The volume ratio of water = 5:1.5:100.

[0089] 3. Add 5 ml of 0.1% Tween 20 aqueous solution (mass volume ratio, Tween 20: water = 0.1 g: 100 ml) to the cultured Neurospora crassa plate, and add sterilized glass beads (such as 10 ), gently shaking for a while, t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for degrading cake by fungi and its application. The preparation method of the biological medium obtained by transforming rapeseed cake with Neurospora is characterized in that it includes the following steps: 1). Drying and sterilizing the remaining rapeseed cake after oil extraction; 2). Inoculating rough Neurospora; 3). Make a spore suspension; 4) press the ratio of the spore suspension and the sterilized rapeseed cake to be 1000ml: 500g, cultivate; 5) then add water for hydrolysis; 6) ferment the hydrolyzate After filtration, the supernatant is sterilized and centrifuged to make a hydrolyzate; the above hydrolyzate is added with water and molasses to obtain a biological culture medium; the amount of water added is 1 to 14 times the volume of the hydrolyzate made, The amount of molasses added is 0% to 50% of the volume of the hydrolyzed solution. The preparation method is simple and environmentally friendly, and can significantly reduce the production cost. The biomass of the thalline of the invention is higher than that obtained under the same conditions of common basic culture medium, and the fat content of the thallus is also obviously increased.

Description

technical field [0001] The invention relates to a method for using fungi to degrade cake (to obtain a degradation product, ie a biological medium) and its application. Background technique [0002] Rapeseed cake is the residue after oil extraction of rapeseed. The annual output of rapeseed cake in my country is about 7 million tons. The proportion of total protein in rapeseed cake is about 35-45% (mass), and it is a complete protein, and there is almost no limiting amino acid. Rapeseed cake can provide more calcium, iron, manganese, phosphorus, selenium and magnesium; it also contains more vitamins such as choline, biotin, niacin, vitamin B1 and B2. At present, at home and abroad, rapeseed cake is the most common utilization method as a feed protein source for livestock, accounting for 5-10% of the output of rapeseed cake. Rural areas often use it as organic fertilizer, which causes a great waste of this natural protein resource. Rapeseed meal is an excellent protein reso...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/845C12R1/645C12R1/89
Inventor 龚阳敏胡传炯万霞江木兰刘姣梁焯黄凤洪胡小加
Owner OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap