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A kind of tissue culture method of Kambha Gabu

A technology of tissue culture and kampagabu, applied in the biological field, can solve the problems of increase and shortage of wild species, and achieve the effect of meeting large-scale production, shortening the breeding cycle, and protecting the ecological environment

Active Publication Date: 2016-08-24
西藏自治区高原生物研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are more and more products with this as the main ingredient, and the demand is greatly increased. At present, there are no cultivated species, and all raw materials are wild, thus causing the shortage of wild species

Method used

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  • A kind of tissue culture method of Kambha Gabu
  • A kind of tissue culture method of Kambha Gabu

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Embodiment 1

[0034] Embodiment 1: Kanba Gabu tissue culture test

[0035] Step 1. Disinfection of materials: Disinfect the Kambagabu seeds collected in the field with 75% alcohol for 30 seconds in the laboratory, rinse with sterile water for 3 times, and then disinfect with 0.1% mercury liter for 5-12 minutes , Rinse 3-5 times with sterile water, both can be used for inoculation.

[0036] Step 2. The medium for the sterilized material is MS medium + 30g / L sucrose + 6g / L agar, + 0.1g / L inositol, pH5.8; the culture condition temperature is 22±2°C, and the light is 12- 14 hours / day, it can germinate after 15 days of cultivation;

[0037] Step 3, differentiation culture: transfer the above-mentioned seedlings after seed germination into 1. MS medium+30g / L sucrose+6g / L agar+0.1g / L inositol, pH5.8 after three weeks; 2. MS medium + 0.5mg / L 6-BA + 30g / L sucrose + 6g / L agar + 0.1g / L inositol, pH5.8; 3. MS medium + 1mg / L 6-BA + 30g / L L sucrose+6g / L agar+0.1g / L inositol, PH5.8; 4. MS medium+2mg / L ...

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Abstract

The invention relates to a technique for rapid propagation of Kambagabu plants, in particular to a method for tissue culture of Kambagabu plants. Specifically, by selecting a specific tissue culture method, especially a differentiation medium and a rooting medium for tissue culture, a method for rapid propagation of tissue culture suitable for medicinal Kambagabu plants is finally provided. According to the method of the invention, the survival rate of the transplanted seedlings reaches more than 90%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a rapid propagation technology of a Kambagabu plant, in particular to a method for tissue culture of a Kambagabu plant. technical background [0002] Kambagabu (scientific name: Artemisia vestita Wall.ex DC) belongs to Compositae and Artemisia. Semi-shrub or shrubby; rhizome thick, woody, plant has volatile aroma. The main products are Dingqing, Changdu, Jiangda, Chaya, Gongjue, Luolong, Basu, Zuogong, Zayu, Bomi, Milin, Jiacha, Langxian, Longzi, Luozha, Lhasa, Nanmulin, Shigatse. It grows at an altitude of 2600-4300 meters, on hillsides, foothills, grasslands, shrubs, gravel beaches, etc. Used as medicine, it has the effects of clearing away heat, detoxification, promoting dampness and expelling wind. It is also used as fodder in pastoral areas. [0003] The Tibetan medicinal material "Kanbagabu" is an incense and disinfectant plant that is often kept in Tibetan homes. According ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 泽仁旺姆尼珍郭尚磊
Owner 西藏自治区高原生物研究所
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