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Method for governing pollution in micro-algae cultivation process

A technology of microalgae cultivation and microalgae, applied in the direction of chemistry, can solve the problems of reducing the bactericidal effect, easy decomposition, carcinogenicity, etc., and achieve the effect of stable bactericidal effect, strong adsorption and penetration ability, and high bactericidal efficiency.

Inactive Publication Date: 2014-10-01
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the process of pollution control, the most used drug is sodium hypochlorite, but sodium hypochlorite has the following disadvantages: (1) sodium hypochlorite is greatly affected by the environment, unstable, and easy to decompose, so it must be used under a certain temperature, light and pH In the microalgae culture process, the pH of the culture system will fluctuate with the growth of algae cells, and the growth of algae cells can only grow under the condition of light, so sodium hypochlorite (2) The bactericidal effect of sodium hypochlorite is greatly affected by organic matter, and as the cultivation time continues to grow, extracellular secretions will appear in the culture solution, and the amount of extracellular secretions There is a large proportion of polysaccharides in the ingredients, and this part of the extracellular secretion will greatly reduce its bactericidal effect; (3) During the use of sodium hypochlorite, the free chlorine released may cause poisoning of operators, and has carcinogenic, teratogenic, and carcinogenic effects. mutation triple effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Nannochloropsis was cultured in the same manner as in the comparative example. When the culture system is slightly polluted, the following methods should be adopted for treatment:

[0062] Add chlorine dioxide to the algae liquid, adjust its available chlorine content to be 0.2ppm, continue to cultivate, and this concentration has quickly controlled the pollution; observe the growth of Nannochloropsis, under this concentration, chlorine dioxide has no effect on Nannochloropsis. Microdamages were generated, but the damage was repairable, and Nannochloropsis quickly completed its own repair. After the cultivation, the algae mud was collected and the yield was calculated.

[0063] After the breeding is all over, the reactors related to the breeding are disinfected, specifically: the reactors are disinfected with 8 ppm chlorine dioxide for 24 hours.

[0064] In the comparative example, 0.2ppm sodium hypochlorite was used for treatment when slight pollution occurred, and t...

Embodiment 2

[0067]Nannochloropsis was cultured in the same manner as in the comparative example. When moderate pollution occurs in the culture system, the following methods should be adopted for treatment:

[0068] Add chlorine dioxide to the algae liquid, adjust its available chlorine content to be 3ppm, and continue culturing. After 24 hours, stop the ventilation, let stand, remove the residues such as the processed pollutants and a small amount of killed Nannochloropsis that settled at the bottom of the reactor, and supplement fresh medium to continue the culture. Observing the growth of Nannochloropsis, it can be found that at this concentration, chlorine dioxide has slightly damaged Nannochloropsis, but in supplemented fresh medium, Nannochloropsis recovered to the maximum extent. active. After the cultivation, the algae mud was collected and the yield was calculated.

[0069] After the breeding is all over, the reactors related to the breeding are disinfected, specifically: the r...

Embodiment 3

[0073] Nannochloropsis was cultured in the same manner as in the comparative example. When the culture system is heavily polluted, the following methods should be adopted for treatment:

[0074] Add chlorine dioxide to the algae liquid, adjust its available chlorine content to be 12ppm, and continue to cultivate. After 24 hours, stop the ventilation, let stand, remove the residues such as the processed pollutants and a small amount of killed Nannochloropsis that settled at the bottom of the reactor, and supplement fresh medium to continue the cultivation. Observing the growth of Nannochloropsis, it can be found that at this concentration, chlorine dioxide has caused greater damage to Nannochloropsis, but after supplementing with fresh medium, Nannochloropsis has completed the damage after 24 hours. Restoration, and because the processed pollutants and a small amount of killed Nannochloropsis and other residues are removed, the restored Nannochloropsis maximizes biological pro...

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Abstract

The invention discloses a method for governing pollution in a micro-algae cultivation process, and relates to the technical field of micro-algae cultivation. The method is stable in sterilization effect and is little affected by temperature and illumination in the pollution control process; high sterilization efficiency is kept within the pH range of 2-10; the sterilization efficiency is not affected by extracellular secretions of frustules. The method is free of stimulation on a human body, and non-toxic; three cancerogenic substances and other toxic substances are not generated. The method for governing pollution in the micro-algae cultivation process, which is disclosed by the invention, comprises the following steps: adding chlorine dioxide to an algae solution, and sterilizing a reactor by using the chlorine dioxide after cultivation is ended. The method for governing pollution in the micro-algae cultivation process, which is disclosed by the invention, is applicable to a liquid cultivation process of the micro-algae.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a method for controlling pollution in the microalgae cultivation process. Background technique [0002] Microalgae are a kind of autotrophic plants widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. The polysaccharides, proteins, pigments, etc. produced by cell metabolism make them useful in the fields of food, medicine, genetic engineering, and liquid fuels. Very good development prospects. [0003] In the process of microalgae cultivation, with the increase of the cultivation period and batches, it is inevitable that there will be pollution. At present, in the process of pollution control, the most used drug is sodium hypochlorite, but sodium hypochlorite has the following disadvantages: (1) sodium hypochlorite is greatly affected by the environment, unstable, and easy to decompose, so it must be used under a certain ...

Claims

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Application Information

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IPC IPC(8): A61L2/18A61L101/06
Inventor 韩春梅王琳陈昱张惠敏李青王冰吴洪
Owner ENN SCI & TECH DEV
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