A kind of polymethacrylamide cationic polymer synergistically modified by side group natural arginine and lactobionic acid, preparation method and use
A polymethacrylamide cation and polymethacrylamide technology, which is applied in the field of biological functional materials, can solve the problems of reducing cell membrane damage ability, reducing cation charge density, etc., and achieves reduced interaction and surface adsorption, low cytotoxicity, The effect of improving gene transfection efficiency
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Embodiment 1
[0026] The first step: the RAFT reagent 4-cyano-4-(dodecyltrithiocarbonic acid) valeric acid (35 mg), the hexamethylenediaminomethacrylamide functional monomer protected by the terminal amino Boc Azobisisobutyronitrile AIBN (2.8 mg) free radical initiator and 6 mL of dry tetrahydrofuran solvent were added to the Schlenk reaction tube. After 3 cycles of freezing-vacuumizing-thawing and nitrogen circulation to remove residual oxygen in the reaction solution, keep the reaction tube under a nitrogen atmosphere, move it into an oil bath preheated to 70°C and stir for 6 hours. Then the reaction was rapidly quenched with liquid nitrogen, and the reaction solution was concentrated by rotary evaporation under reduced pressure. The concentrated solution was precipitated in glacial ether to obtain a light yellow solid, which was filtered and dried in a vacuum oven for 24 hours to prepare hexamethylenediamine-terminated amino-protected Boc The methacrylamide polymer, conversion rate is 6...
Embodiment 2
[0033] The first step: the RAFT reagent 4-cyano-4-(dodecyltrithiocarbonic acid) valeric acid (70mg), the hexamethylenediaminomethacrylamide functional monomer protected by the terminal amino Boc Azobisisobutyronitrile AIBN (5.6 mg) free radical initiator and 15 mL of dry toluene solvent were added to the Schlenk reaction tube. After 3 cycles of freezing-vacuumizing-thawing and nitrogen circulation to remove residual oxygen in the reaction solution, keep the reaction tube under a nitrogen atmosphere, move it into an oil bath preheated to 70°C and stir for 6 hours. Then the reaction was rapidly quenched with liquid nitrogen, and the reaction solution was concentrated by rotary evaporation under reduced pressure. The concentrated solution was precipitated in glacial ether to obtain a light yellow solid, which was filtered and dried in a vacuum oven for 24 hours to prepare hexamethylenediamine-terminated amino-protected Boc The methacrylamide polymer, the conversion rate is 68%. ...
Embodiment 3
[0040] The first step: the RAFT reagent 4-cyano-4-(dodecyltrithiocarbonic acid) valeric acid (35 mg), the hexamethylenediaminomethacrylamide functional monomer protected by the terminal amino Boc Azobisisobutyronitrile AIBN (2.8 mg) free radical initiator and 6 mL of dry tetrahydrofuran solvent were added to the Schlenk reaction tube. After 3 cycles of freezing-vacuumizing-thawing and nitrogen circulation to remove residual oxygen in the reaction solution, keep the reaction tube under a nitrogen atmosphere, move it into an oil bath preheated to 70°C and stir for 6 hours. Then the reaction was rapidly quenched with liquid nitrogen, and the reaction solution was concentrated by rotary evaporation under reduced pressure. The concentrated solution was precipitated in glacial ether to obtain a light yellow solid, which was filtered and dried in a vacuum oven for 24 hours to prepare hexamethylenediamine-terminated amino-protected Boc The methacrylamide polymer, conversion rate is 6...
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